The capacity intermittent titration technique (CITT) was developed based on the ratio of potentio-charge capacity to galvano-charge capacity (RPG) method, to continuously determine the solid diffusion coefficient (D) of the intercalary species within insertion-host materials with a small voltage region. The linear equations of D vs the value of ratio of the potentio-charge capacity to the galvano-charge capacity (q) were given. By the CITT technique, the Li⁺ solid diffusion coefficients within LiMn₂O₄ at different voltages were determined. The results show that the values of D varied from 3.447 × 10⁻⁹ to 7.60 × 10⁻¹¹ cm²/s in the voltage range of charge from 3.3 to 4.3 V as a function of voltage with “W” shape.

Rare earth-doped WC-Co cemented carbides with low carbon-containing level were prepared by adding mischmetal (mainly containing lanthanum and cerium) in the form of cobalt pre-alloyed powder during ball-milling. After sintering, the sinter skin (exterior) was observed and analyzed by scanning electron microscopy (SEM) and energy disperse X-ray spectroscopy (EDXS). The results show that during the sintering process lanthanum and cerium are separated and cerium predominantly migrates towards the surface forming precipitates containing oxygen. Thermodynamic analysis and polarization of cerium atom caused by the strong interaction between cerium atoms and high energy carbon atoms were used in explaining the observed phenomenon.

To evaluate the biocompatibility of MIM 316L stainless steel, the percentage of S-period cells were detected by flow cytometry after L929 incubated with extraction of MIM 316L stainless steel, using titanium implant materials of clinical application as the contrast. Both materials were implanted in animal and the histopathological evaluations were carried out. The statistical analyses show that there are no significant differences between two groups (P>0.05), which demonstrates that MIM 316L stainless steel has a good biocompatibility.

In order to obtain optimizing microarc oxidation coating on Mg alloy from a friendly-enviormental electrolyte free of Cr⁶⁺ and PO₄³⁻, constant potential regime was applied to produce it. The influence of potential on the morphology, composition, structure and other properties, such as microhardness and corrosion resistance were investigated by scanning electron microscopy (SEM), energy dispersive spectroscope (EDS), X-ray diffraction (XRD), hardness tester and electrochemical method. The results clearly show that oxidation potential plays an important role in the formation of coating’s structure and properties. The microarc oxidation coating is smooth and white, which consists of two layers. The external layer is loose and porous and enriched in Al and Si. Moreover, its content of Al and Si increases with the increasing operated potential. While the inner layer is compact and the content of Al and Si are lower than that of the external layer. The coating is composed of several phases and the major phases are MgAl₂O₄ and MgO, and the minor phases are Al₂O₃ and SiO₂ when the potential is higher. The microhardness of coating is obtained the maximum at the potential of 45 V, so does the corrosion resistance.

Niobium suboxide powder was pressed and sintered in vacuum into NbO electrolytic capacitor sintered anode. High voltage and constant current formation experiment was performed on NbO electrolytic capacitor anode, during which electrolyte was 0.01% H₃PO₄ solution, temperature was 90 °C and current was 50 mA per gram sample. Through the relationship between anode voltage and time and scanning electron microscopy(SEM) images of invalidated anode and normal forming anode, invalidation manner and mechanism of NbO electrolytic capacitor anode were discussed. The results show that, the main invalidation manner of NbO electrolytic capacitor anode is not short circuit but open circuit, which is different to that of traditional Ta electrolytic capacitor anode. The reason of invalidation is that anode oxide film whose thickness increases gradually penetrates the “connection neck’ among anode powder particles, which leads to the open circuit invalidation of anode. Compared with Ta electrolytic capacitor, NbO electrolytic capacitor has better security.

Mechanical properties and microstructure of Al-Mg-(Sc, Zr) welded joint prepared by manual labor melt inert gas(MIG) welding method using Al-Mg and Al-Mg-Sc weld wire as filling materials were studied comparatively. The results show that the apparent recrystallization does not happen in heat-affected zone. It proves that Al-Mg(Sc,Zr) alloy has a high capability of resistance to weldiing heat-soften. Using Al-Mg-Sc welding wire as filling material can obviously refine the grains of weld seam and improve its strength. The layer of small equal-axe grains in fusion area improves the joint strength between welded metal and base metal. The coefficients of welded joints when Al-Mg-Sc and Al-Mg weldiong wire are used as filling material are greater than 0.90, respectively. The yield strength of welded joints with Al-Mg welding wire is only 187 MPa, one is as 287 MPa with Al-Mg-Sc welding wire, thus the working strength of the welded unit is greatly improved.

The spinel LiMn₂O₄ used as cathode materials for lithium-ion batteries was synthesized by mechano-chemistry fluid activation process, and modified by doping rare-earth Sm. Thesting of X-ray diffraction, cyclic voltammograms, charge-discharge and SEM was carried out for LiMn₂O₄ cathode materials and the modified materials. The results show that the cathode materials doped rare earth Li_xMn_2−ySm_zO₄ (0.95⩽x⩽1.2, 0⩽y⩽0.3, 0⩽z⩽0.2) exhibit standard spinel structure, high reversibility of electrochemistry and excellent properties of charge-discharge. In EC: DMC(1 : 1)+1 mol/L LiPF₆ electrolyte with discharge capacity more than 130 mA · h/g, and its capacity is deteriorated less than 15% after 300 cycles at room temperature and less than 20% after 200 cycles at 55°C. At the same time, Crystal Field Theory was applied to explain the function and mechanism of doped rare earth element.

The factors affecting the dimensional tolerance of powder injection molding (PIM), such as the selection of the powder and binder, the feedstock homogeneity, the feedstock thermal properties, the feedstock rheologic behavior, the debinding schedule and atmosphere and sintering temperature gradient were discussed. An attempt was made to develop a model to estimate the influence of important variables. The results show that a better understanding of these factors can provide some useful theoretical instructions for large scale production.

The availability using oxygen-rich powders to prepare ultrafine Ti(C,N)-based cermets was investigated. The deoxidation process, denitrification phenomenon and the effect of deoxidation on microstructure and mechanical properties of sintered samples were discussed, respectively. The results show that oxygen in the samples prepared even with high oxygen contained in starting powders can be almost completely cleaned away through suitable sintering process. The ultrafine oxygen-rich powders have a significant effect on microstructure, which promotes the formation of white core phase. A ultrafine Ti(C,N)-based cermet with mean particle size of 0.30 µm, uniform microstructure and excellent mechanical properties is successfully prepared. It is also found that there exists severe denitrification phenomenon in the preparation process of ultrafine Ti(C,N)-based cermet.

LiNi_0.45Co_0.10Mn_0.45O₂ was synthesized from Li₂CO₃ and a triple oxide of nickel, cobalt and manganese at 950 °C in air. The structures and characteristics of LiNi_0.45Co_0.10Mn_0.45O₂, LiCoO₂ and LiMn₂O₄ were investigated by XRD, SEM and electrochemical measurements. The results show that LiNi_0.45Co_0.10Mn_0.45O₂ has a layered structure with hexagonal lattice. The commercial LiCoO₂ has sphere-like appearance and smooth surfaces, while the LiMn₂O₄ and LiNi_0.45Co_0.10Mn_0.45O₂ consist of cornered and uneven particles. LiNi_0.45Co_0.10Mn_0.45O₂ has a large discharge capacity of 140.9 mA · h/g in practical lithium ion battery, which is 33.4% and 2.8% above that of LiMn₂O₄ and LiCoO₂, respectively. LiCoO₂ and LiMn₂O₄ have higher discharge voltage and better rate-capability than LiNi_0.45Co_0.10Mn_0.45O₂. All the three cathodes have excellent cycling performance with capacity retention of above 89.3% at the 250th cycle. Batteries with LiMn₂O₄ or LiNi_0.45Co_0.10Mn_0.45O₂ cathodes show better safety performance under abusive conditions than those with LiCoO₂ cathodes.

The deformation behavior of dispersion strengthened copper alloy Cu-Al₂O₃ was studied by TEM. The results show that nano-scaled dispersed second phase not only increases dislocation density in matrix, but also has an important influence on the dislocation substructure. The presence of fine dispersed Al₂O₃ particles results in a uniform and random dislocation distribution in matrix copper and causes the difficulty in formation of dislocation cell structure and the decrease in the amount of cell structure during deformation. Deformation gives rise to much more dislocations and dislocation cells form more difficultly and the decrease in the cell size with the increase of dispersion degree.

LiNi_0.5Mn_1.5O₄ was prepared under various conditions by one-step solid-state reaction in air and its properties were investigated by X-ray diffractormetry (XRD), scanning electron microscopy (SEM) and electrochemical measurement. XRD patterns show that LiNi_0.5Mn_1.5O₄ synthesized under various conditions has cubic spinel structure. SEM images exhibit that the particle size increases with increasing calcination temperature and time. Electrochemical test shows that the LiNi_0.5Mn_1.5O₄ calcined at 700 °C for 24 h delivers up to 143 mA · h/g, and the capacity retains 132 mA · h/g after 30 cycles.

TiO₂ powder and TiO₂ thin film on the surface of glazed ceramic tile were prepared by sol-gel method. The influences of different doping Cr³⁺ concentration on the photocatalytic activity of TiO₂ were discussed, UV-visible and X-ray diffraction analysis were used to test the performance of TiO₂ powder and film. The results indicate that photocatalytic activity of doping Cr³⁺-TiO₂ thin film is higher than that of powder, and the interaction between Cr³⁺-doped and substrate can greatly enhance the photocatalytic activity. The results of X-ray diffraction and photoabsorption show that the Cr³⁺-doped energy level in TiO₂ is 0.62 eV high from the top of valence band, which belongs to the type of deep energy level doping. On the basis of the semiconductor energy level theory and Cr³⁺ dopant energy level, the semiconductor energy level model of Cr³⁺ in TiO₂ powder and thin film were established, and the doping mechanisms of Cr³⁺-doped in TiO₂ powder and thin film were analyzed.

The thermal behaviors of the deposits on supported noble metal catalysts for synthesizing 2, 3, 5-trimethylhydroquinone by DSC were studied. The results show that the supported Pd catalysts have two exothermic peaks at around 312 °C and 435 °C in the DSC thermograms, respectively, while the supported Pt catalysts have one exothermic peak at approximately 345 °C. Therefore, it is supposed that the adsorptive states of deposits on supported Pt catalysts and supported Pd catalysts are different, which may be one reason leading to the stability difference between supported Pt catalysts and supported Pd catalysts.

A kind of complex additive mainly containing Al, Mg, F, and O was prepared. The synthetical performances of the property-modified prebaked anodes containing additives were tested in laboratory. On the basis of ideal testing results obtained, a large number of industrial prebaked property-modified anodes are prepared in a large-scale aluminum company. Further more, they are all used in 160 kA prebaked anode aluminum electrolysis cells. The statistic result show that, compared with common anodes, the property-modified ones enhance current efficiency by 0.78%, reduce energy consumption by 106.1 kW·h per ton aluminum and reduce carbon consumption by 11.6 kg per ton aluminum averagely.

Exchange current density of spinel LiMn₂O₄ was studied by linear polarization. The relationship of the kinetic property with the structure of spinel LiMn₂O₄ was investigated by studying the effect of the doping and surface coating on the kinetic properties of electrode material. The results show that the exchange current density of spinel LiMn₂O₄ electrode increases with the increase of the amount for lithium intercalation at first, and then decreases. The maximal exchange current density appeares at the 80%–90% lithium intercalation. The similar phenomenon was observed on the doped spinel LiMn₂O₄ electrode. Doping can enhance the exchange current density of spinel LiMn₂O₄ material. However, the degree of the doping effect varies with the doped element varying. Surface coating can also enhance the exchange current density of spinel material, and the increment of value is higher than that of doped ones.

The AAAc(1 : 1) was synthesized in water by As₂O₃ and Sb₂O₃ with molar ratio of 1 : 1. AAAc(1 : 1) was characterized by Raman, IR, TG/DTG, DSC, XPS and XRD. The results show that there are four peaks to v_s of As-OH, As-O-Sb, Sb-OH and Sb-O-Sb in Raman spectra of AAAc(1 : 1) at 100 – 1 000 cm⁻¹. The solution of AAAc(1 : 1) was also titrated with KOH solution. The titration results show that AAAc(1 : 1) is a hexabasic acid with dissociation constants of k₁=3.62 × 10⁻², k₂=3.05 × 10⁻³, k₃=6.43 × 10⁻⁶, k₄ =9.78 × 10⁻⁸, k₅=1.32 × 10⁻¹¹, k₆=3.87 × 10⁻¹². AAAc(1 : 1) has a good solubility and stability in water, its solid obtained by free volatilizing water from its solution under air at ambient temperature is amorphous. Chemical and thermal analysis show that the composition of AAAc(1 : 1) is As₂O₅ · Sb₂O₅ · 8H₂O in air at 25 °C. AAAc(1 : 1) has the structure of AsO(OH)₂-OH-Sb(OH)₄-O-Sb(OH)₄-OH-AsO(OH)₂ or As(OH)₃-O-Sb(OH)₄-O-Sb(OH)₄-O-As(OH)₃ (isomerism) through experimental determination and geometry optimization.

Electroless copper plating process of N, N, N′, N′-tetrakis (2-hydroxypropyl) ethylenediamine (THPED) chelating agent was researched comprehensively. The results indicate that plating rate decreases with the increase of concentration for THPED, CuSO₄ · 5H₂O and HCHO, pH value and bath temperature. The additive of K₄[Fe(CN)₆] · 3H₂O, 2, 2′-dipyridyl and polyethylene glycol(PEG) decrease plating rate and K₄[Fe(CN)₆] · 3H₂O has a bad effect on deposits quality, but 2, 2′-dipyridyl and PEG make deposits quality improve greatly. Low concentration of 2-mercaptobenzothiozole (2-MBT) increases plating rate and improves deposits quality, but decreases plating rate and worsens deposits quality when 2-MBT reaches 5 mg/L. The optimal conditions of this electroless copper plating process are that the concentration of THPED, HCHO, CuSO₄ · 5H₂O, PEG, 2, 2′-dipyridyl and 2-MBT are 16.8 g/L, 16.0 mL/L, 13.3 g/L, 0.5 g/L, 5.0 mg/L and 2.0 mg/L, respectively, pH value is 12.75, bath temperature is 30 °C. Plating rate reaches 9.54 µm/h plating for 30 min in the bath. The SEM images demonstrate that the surface of copper film is smooth and the crystal is fine.

Based on the data and methods provided by research literature, dispersing mathematical model of combustion process of asphalt smoke is set by theoretic analysis. Through computer programming, the dynamic combustion process of asphalt smoke is calculated to simulate an experimental model. The computing result shows that the temperature and the concentration of asphalt smoke influence its burning temperature in approximatively linear manner. The consumed quantity of fuel to ignite the asphalt smoke needs to be measured from the two factors.

A close-looped process based on the membrane separation and electrolysis is proposed to regenerate the copper etchant in-situ, recover copper on-site and reuse it. It is characterized by selective separation of copper from the spent etchant, which is accomplished by the ion exchange membrane-electrowinning, and at the same time the other components useful for etching are reclaimed. The experiments show that at least 90% of electricity efficiency for copper removal can be maintained and the optimum condition for membrane-electrowinning is: cell voltage 2–2.5 V, operating temperature 40–50 °C and current density 500–1500 A/m². The regenerated etchant can be successfully reused to etch copper after adjusting its composition to the normal range, and its recycling property is as good as that of the fresh etchant after 50 times of use-disposal-regeneration cycles.

The influence of coal desulfurization by fungus was experimentally studied. The results suggest that fungus can effectively remove inorganic and organic sulfur in coal, and main influences of desulfurization by fungus of pH value, temperature, coal slurry concentration and coal granularity were studied by orthodox experiment and the optimal experimental conditions are as follows: pH value 6, temperature 45 °C, coal slurry concentration 10% and coal granularity 100 µm. Under above conditions, fungus can remove up to 44.96% total sulfur and 54.87% inorganic sulfur within two days, and their desulfurization rates will increase along with time. Compared with sulfolobas, desulphurization by fungus is steady and more effective, and has advantage of high speed.

A molecularly imprinting polymer (MIP) was synthesized via bulk polymerization under different conditions using anti-ague drug cinchonine (CN) as template. Infrared spectra (IR) results show that the template CN and functional monomer α-methyl acrylic acid (MAA) formed complexes before polymerization and the structure of complexes was simulated by Hyperchem. The results indicate that there are hydrogen bond or ionic bond between functional monomer and template molecule in acetonitrile solution. The MIP made in cold-initiated photo-polymerization has higher separation performance than that in the therm-initiated polymerization. The separation of the isomers CN and cinchonidine (CD) can be successfully obtained when its separate factor α reaches 1.82. Scatchard analysis suggests that the MIP recognizing CN with two classes of binding sites. The partition coefficient K_{d, 1} and apparent maximum number n_{max, 1} of binding sites with high affinity are 131.43 µmol/L and 58.90 µmol/g, respectively, while K_{d, 2} and n_{max, 2} of binding sites with low affinity are 2.32 mmol/L and 169.08 mmol/g, respectively.

Copper-bearing deposits of China are statistically analyzed in terms of ore grade, metal amount and ore tonnage. Each of grade and metal amount shows more or less a lognormal distribution. Analysis gives 10 copper metallogenic districts, each having specific densities of deposit numbers and copper reserves larger than 3. Based on the ratio of copper in ore value (R_Cu), Chinese copper deposits are classified into two groups: mainly copper-producing deposits (MC: R_Cu⩾0.5) and accessorily copper-producing deposits (AC: R_Cu<0.5). The grade-tonnage relation of MC deposits can be combined by two exponential functions approximating high grade (>3.0%) and low grade (<2.0%) parts. The critical copper grade, which is obtained from the low grade part of the relation, is 0.34%. Chinese copper resources are concluded to become pessimistic, because some mines are working with grades close to this critical value. Taking account of the fact that many copper deposits are actually polymetallic, Cu-equivalent grades, which are converted from ratios of metal prices to the copper price, are also introduced. The critical Cu-equivalent grade of MC deposits (0.43%) also suggests that Chinese copper resources are pessimistic.

The thermal conductivity of rock is an important parameter for the deep mine and the geothermal development. It is often not possible to measure the thermal conductivity of the rocks present in the deep strata, and the usual approach is to calculate thermal conductivity including mineralogy and porosity. The compositions of core samples from the MID01 borehole in the Björkö area were determined, and the minera composition was classified. The calculation of the thermal conductivity of rock in the borehole was carried out, and the main factors for the thermal conductivity of rock were analyzed. The results show that the calculated thermal conductivity of rock is reliable and useful for the design and calculation of geothermal development in the Björkö area.

Lining craze, as a common engineering damage, affects the bearing capacity and the safety of tunnels. To improve the capabilities of the surface crack detection in the tunnel lining, ground penetrating radar (GPR) was employed. The principle and method of GPR for cracks investigation of tunnel lining were expounded. As an application example, some field measurements in order to detect the depth of cracks for the tunnel lining were achieved in a new tunnel. All GPR investigations were made with the antennae of 1 GHz. The cracks of concrete structure were located, and the distributing and depth of cracks are inspected and estimated by GPR. The results of investigation show GPR is accurate and efficient to detect cracks of tunnel lining.

Anti-plane punch-through shear test and anti-plane four-point bending test are used to study the crack initiation and propagation under anti-plane shear (Mode III) loading. The tensile and shear stresses at the crack tip are calcualted by finite element method. The results show that under Mode III loading the maximum principal stress σ₁ at crack tip is smaller or a little larger than the maximum shear stress τ_max. Since the tensile strength of brittle rock is much lower than its shear strength, σ₁ is easy to reach its critical value before τ_max reaches its critical value and thus results in Mode I fracture. The fracture trajectory is helicoid and the normal direction of tangential plane with the fractured helicoid is along the predicted direction of the maximum principal stress at the notch tip. It is further proved that Mode I instead of Mode III fracture occurs in brittle rock under Mode III loading. The fracture mode depending on the fracture mechanism must be distinguished from the loading form.

Longtoushan Sn-polymetal deposit is a large-scale deposit of high-tenor. The ore-bodies occur in reef limestone of middle Devonian. There is much anthraxolite in reef limestone and ore-bodies. The anthraxolite is the postmature result of oil-gas’ thermal metamorphism. The close relationship of anthraxolite and Sn-polymetal deposit reveals the space-time relation between oil-gas evolution and Sn-polymetal mineralization. Sulfur isotope of Longtoushan deposit is close to oil’s sulfur in Devonian, which indicates obvious relationship between the sulfur’s source of deposit and oil-gas’ activity. The forming of Longtoushan deposit relates to exhalative-sedimentary mineralization in Devonian. Because of the favorable hydrocarbon-forming condition of Longtoushan reef and surrounding basin facies’ black shale and peat, coupling of ore-formation and hydrocarbon-forming occurs in seabed’s hydrothermal convection. The distributing of ore-forming elements indicates the presence of hydrothermal convection system. The thermal fluid containing organic matters conduces to Sn-polymetal elements’ activation and transfer, and provides catalyzing condition to the transforming from SO₄²⁻ to S²⁻. The erosion action of brine containing organic acid to reef limestone induces the growing of crannies and karst’s caverns, which provides advantageous space to Sn-polymetal mineralization. The heat source of mineralization provides thermocatalysis condition to hydrocarbon-forming. When the circulatory fluid containing oil-gas enters the high-temperature region(>150°C), the oil-gas is decomposed and anthraxolite comes into being.

Blast vibration analysis constitutes the foundation for studying the control of blasting vibration damage and provides the precondition of controlling blasting vibration. Based on the characteristics of short-time non-stationary random signal, the laws of energy distribution are investigated for blasting vibration signals in different blasting conditions by means of the wavelet packet analysis technique. The characteristics of wavelet transform and wavelet packet analysis are introduced. Then, blasting vibration signals of different blasting conditions are analysed by the wavelet packet analysis technique using MATLAB; energy distribution for different frequency bands is obtained. It is concluded that the energy distribution of blasting vibration signals varies with maximum decking charge, millisecond delay time and distances between explosion and the measuring point. The results show that the wavelet packet analysis method is an effective means for studying blasting seismic effect in its entirety, especially for constituting velocity-frequency criteria.

Forecasting subtle traps by sequence stratigraphy and 3D seismic data is a sensitive topic in hydrocarbon exploration. Research on subtle traps by geophysical data is the most popular and difficult. Based on the sufficiently drilling data, log data, core data and 3D seismic data, sediment sequence of Qikou depression, Huanghua basin was partitioned by using sequence stratigraphy theory. Each sediment sequence system mode was built. Sediment faces of subtle traps were pointed out. Dominating factors forming subtle traps were analyzed. Sandstone seismic rock physics and its response were studied in Tertiary System. Sandstone geophysical response and elastic modulus vary laws with pressure, temperature, porosity, depth were built. Experimental result and practice shows that it is possible using seismic information forecasting subtle traps. Integrated using geology, log, drilling data, special seismic processing technique, interpretation technique, high precision horizon calibration technique, 3D seismic visualizing interpretation, seismic coherence analysis, attribute analysis, logging-constrained inversion, time frequency analysis, subtle traps object is identified and interpreted. Finally, advantage object of subtle trap in this area was determined. Bottomland sand stratigraphic and lithologic reservoirs in Qinan slope zone have been founded by means of high resolution 3D seismic data field technique, high resolution 3D seismic data processing technique and seismic wave impedence inversion technique.

New method for handling roof of the base successive mining is proposed, which is induction caving in the roof. The key is that it is made certain to the station of the space-time in the induction caving roof, as the stress is released with the mining process. And applying the catastrophe theory, the influencing factors of induction caving roof are studied in the emptied areas, such as the mechanical property of the surrounding rock, the area of the gob, the scope and dimension of tensile stress. The results show that the key factor is the area of the gob to the method of the induction caving roof. Then according to the geology and the ore characteristic, the three dimension FEM mechanical model is built in Tongkeng Mine, the laws of the tensile stress are analyzed to the space and the time in the roof with the mining, then it is rational design to the mine step and time of the handing the roof.

Eastern Tianshan, a cardinal part of Northern Xinjiang compound continental crustoblock, is located in the east of Tianshan Diwa region. By analysis of regional geological and geophysical data, and by using crustobody theory and researching methods, this region can be divided into three sectors of different crustal structure. During Proterozoic to Eopaleozoic, the spreading and closing of Northern Tianshan Sea, on the foundation of disparate continental basements of each sectors, the transversal heterogeneity of tectonic movement and evolution resulted in the diversity of crustal structure and material formations. The metallogenic evolution indicated that different geotectonic evolution periods had not only different characteristics of mineralization commodity and mineral deposit types, but also the progressive mineralization feature. In Neopleozoic, the closing of Northern Tianshan Sea from north to south and the difference of transmeridional tectonic deformation and magmation formed in the movement constituted the different metallogenic environment in shallow crust. Furthermore, it brought on the diverse Cu-Au-polymetal mineral deposit types and metallogenetic epoch from north to south and from west to east.

Similar materials such as cement, gypsum and sand are options for simulating limestone characteristic. A series of reasonable proportions are chosen to do similar experiments of Karst roof based on the proportions testing of small samples indoors. Applying on ultrasonic, the velocities of transverse wave and vertical wave of similar samples have been inspected with the sound wave instrument. Dynamic modulus of elasticity and Poisson’s ratio of the samples have been educed. According to the testing data, the relationship between the transverse wave and vertical wave velocity, compressive strength and anti-bend strength are analyzed. It has been proved that the vertical wave velocity is better for reflecting compressive strength and anti-bend strength of similar materials than the transverse wave velocity. The vertical wave velocity increases with the strength and dynamic modulus of elasticity.

The dynamic impedance function of pile in visco-elastic material considering axial loads under lateral dynamic force was analyzed, and the beam dynamic differential equation was used to induce the dynamic impedance function. After analyzing the edge conditions, the dynamic impedance functions were deduced. Contrasted with the result that does not consider axial loads, the axial loads have obvious influence on the dynamic impedance function. And the results show that the dimensionless parameter of the dynamic impedance will change from 6% to 9% when considering axial loads, and dimensionless parameter of the dynamic impedance of the coupling horizontal-sway will increase by 31%.

Clay-solidified grouting curtains are commonly used for remediation by containment or pollution prevention, in addition to their use as a barrier to water flow in municipal solid waste(MSW) landfills. A hydrological model of water flow and a hydrodynamic model of contaminant are presented to simulate the migration of leachate through clay-solidified grouting curtain in MSW landfills, with particular attention paid to the role of diffusive and adsorptive fluxes in contaminant transport. The models were applied to simulate the sensitivity of the curtain’s behavior to changes in parameters, such as thickness, depth, permeability coefficient, diffusion coefficient, resistance coefficient and concentration, and also to demonstrate the contaminant distribution on the evolution of travel time and offset distance of clay-solidified grouting curtain in landfills. It is found that a part of leachate components stays or is retarded in clay-solidified grouting curtain by precipitate or exchange, the retention rate is closely related to composition of clay-solidified grouting curtain, more than 80%, and the maximum occurs at the cementclay ratio of 2 : 4 under experimental conditions. Contamination distribution is variable on travel time and offset distance, the highest concentration takes place where the contamination intensity is nearest to the pollution resource or takes place at early middle period of transport, and the pollutant attenuates gradually. The results indicate that claysolidified grouting curtain with a proper thickness, a low permeability coefficient and a high resistance coefficient might serve as a sufficiently effective vertical barrier against leachate seepage and contamination migration in MSW landfills.

By analyzing the abrasive theory of concrete diamond sawblade, the proposal that the diamond should be selected by its function in cutting concrete is presented. The part of the big grit diamonds cut rock, and the part of the small grit diamonds improve the wearability of the matrix. The contrast tests are done with different shapes of sawbaldes in split segment, slant “U” slot segment, sandwich segment, turbo segment and three-slot segment. The special shapes of sawblades can improve the effect of cooling and the removing ability of the rock powder. The data of tests show that the efficiency of cutting and the life of sawblades are improved by designing the diamond prescription and using the especial geometry of segment.

Numerical calculation for two integral transforms in 2.5-D transient electromagnetic forward is a difficult and key task, namely, the inverse Fourier transform and the inverse Laplace transform. Some effective algorithms for them were described. Based on the known algorithms in DC resistivity on wave-number distribution and selection, we proposed a principle on how to choose the least wave-number concerning the central-loop transient electromagnetic method. First, observe the behavior of transformation function curve with regard to wave-number in Fourier domain. In the light of its asymptote, ascertain the coverage scope of wave-number. Compared with analytic solution, the least wave-number in Fourier domain can be derived. Furthermore, the Laplace numerical inversion algorithm which needs only a few Laplace variables in pure real domain was also introduced here. The procedure was applied to forward modeling on transient electromagnetic field of a vertical magnetic dipole over uniform half-space to demonstrate them effectiveness and general applicability.

Super-highly reinforced concrete tube in tube structure is a developing structure system of high-rise building. The more reasonable derivation process of the multi-vertical-line-element model stiffness matrix is given. On the premise of pointing out the problems of present multi-spring element model, combined with present multivertical-line-element model for analyzing on shear wall, the model is expanded to spatial one, and the stiffness matrix of which is derived. Combined with hysteretic axial model and hysteretic shear model, it is suitable for columns, wall limbs and beams with all kinds of section form. Some examples are calculated and compared with test results, which shows that the models have relatively good accuracy. On the base of the experimental phenomenon and failure mechanism for tube in tube structure specimen, nonlinear seismic responses analysis program on the basis of the advantaged element model for tube in tube structure is developed. Calculation results are in good agreement with those of the pseudo-dynamic tests and the failure mechanism can be well reflected.

The near crack line analysis method was used to investigate a crack loaded by two pairs of point shear forces in an infinite plate in an elastic-perfectly plastic solid, and the analytical solution was obtained. The solutions include: the unit normal vector of the elastic-plastic boundary near the crack line, the elastic-plastic stress fields near crack line, law that the length of the plastic zone along the crack line is varied with an external loads, and the bearing capacity of an infinite plate with a center crack loaded by two pairs of point shear forces. The results are sufficiently precise near the crack line because the assumptions of the small scale yielding theory have not been made and no other assumption have been taken.

A multistage assessment index set is chosen based on the analysis of building fire safety system, whereby the weight of each index is determined through an analytic hierarchy process; a fuzzy synthetic assessment model for the building fire safety system is constructed, and the quantified result was obtained by using hierarchy parameter judgment. This fuzzy synthetic assessment method can quantify assessment result of the building fire safety system, so that the fire precautions may be accurately adopted, and the serious potential risk may be avoided. The application shows that this method possesses both objectivity and feasibility.

The problem of robust and reliable control design for linear uncertain impulsive systems with both time-varying norm-bounded parameter uncertainty and actuator failures was studied. The actuators are classified into two groups. One set of actuators susceptible to failures is possible to fail, the other set of actuators robust to failures is assumed never to fail. The outputs of the actuator failures are regarded as zero. The purpose is to design the state feedback controller such that, for all admissible uncertainties as well as actuator failures occurring among a prespecified subset of actuators, the plant remains asymptotically stable. A modified algebraic Riccati equation approach was developed to solve the problem addressed and a robust reliable control law was obtained. An numerical example was also offered to prove the effectiveness of the proposed method.

This study analyzes drift phenomena of deterministic crowding and probabilistic crowding by using equivalence class model and expectation proportion equations. It is proved that the replacement errors of deterministic crowding cause the population converging to a single individual, thus resulting in premature stagnation or losing optional optima. And probabilistic crowding can maintain equilibrium multiple subpopulations as the population size is adequate large. An improved niching method using clustering crowding is proposed. By analyzing topology of fitness landscape using hill valley function and extending the search space for similarity analysis, clustering crowding determines the locality of search space more accurately, thus greatly decreasing replacement errors of crowding. The integration of deterministic and probabilistic replacement increases the capacity of both parallel local hill climbing and maintaining multiple subpopulations. The experimental results optimizing various multimodal functions show that, the performances of clustering crowding, such as the number of effective peaks maintained, average peak ratio and global optimum ratio are uniformly superior to those of the evolutionary algorithms using fitness sharing, simple deterministic crowding and probabilistic crowding.

A design method for controllers and a comprehensive stability analysis for an acrobat based on Lyapunov functions are presented. Three control laws based on three Lyapunov functions are designed to increase the energy so as to move the acrobot into the unstable inverted equilibrium position, and solve the problem of posture and energy. The concept of a non-smooth Lyapunov function is employed to analyze the stability of the whole system. The validity of this strategy is demonstrated by simulations.

The limitations in existing measures for absorbing pressure impact in hydraulic systems were summarized in this paper. Based on the forming principle of the oil in a hydrostatic closed pressure chamber, the underlying reasons of the pressure impact were analyzed theoretically, the intrinsic laws that the extent of the pressure impact in hydraulic oil lines are affected by some factors, such as oil elastic modulus, oil line’s geometrical volume, and changing rate of oil volume versus time etc, were discussed. Experimental investigations into pressure impact in all pressure chambers because of shifting were conducted under different working conditions by employing a special experimental system. The effects of shifting time on pressure impact were studied. A new concept with universal meaning, i. e. optimal shifting time, and its characterizing parameter and the methods of shifting at optimal shifting time were also proposed. The results show that shifting time lag Δt is of rationality and maneuverability. The higher the working pressure, the shorter the shifting time.

The mechanism of particle swarm optimization algorithm is studied, and one can draw the conclusion that the best particle found by the swarm falling into local minima is one of the main reasons for premature convergence. Therefore, an improved particle swarm optimization algorithm is proposed. This algorithm selects the best particle with roulette wheel selection method, so premature converging to local optima is avoided. At last, the improved particle swarm optimization algorithm is applied to optimization of time-sharing power supply for zinc electrolytic process. Simulation and practical results show that the global search ability of IPSO is improved greatly and optimization of time-sharing power supply for zinc electrolytic process can bring about outstanding economic benefit for plant.

The robustly asymptotical stability problem for discrete-time nonlinear systems with time-delay was investigated. Positive definite matrix are constructed through Lyapunov functional. With the identity transform, property of matrix inverse and S-procedure, a new sufficient condition independent of the size of time-delay for robust stability of discrete-time nonlinear systems with time-delay is established. With Schur complement, another equivalent sufficient condition for robust stability of discrete-time nonlinear systems with time-delay is given. Finally, a sufficient condition dependent on the size of time-delay for robust stability of discrete-time nonlinear systems with time-delay is obtained. A unified approach is used to cast the robust stability problem into a convex optimization involving linear matrix inequalities.

A general scheduling framework (GSF) for independent tasks in computational Grid is proposed in this paper, which modeled by Petri net and located on the layer of Grid scheduler. Furthermore, a new mapping algorithm aimed at time and cost is designed on the basis of this framework. The algorithm uses weighted average fuzzy applicability to express the matching degree between available machines and independent tasks. Some existent heuristic algorithms are tested in GSF, and the results of simulation and comparison not only show good flexibility and adaptability of GSF, but also prove that, given a certain aim, the new algorithm can consider the factors of time and cost as a whole and its performance is higher than those mentioned algorithms.

The hydraulic flexible manipulator system is divided into two parts: flexible arm dynamics and hydraulic servomechanism, a driving Jacobian is derived to connect these two parts. Taking hydraulic actuator force as virtual input, a singular perturbed composite model is formulated and used to design composite controllers for the flexible link, in which the slow subsystem controller dominates the trajectory tracking, and then a fast controller is designed to damp out the vibration of the flexible structure. Moreover, the backstepping technique is applied to regulate the spool position of a hydraulic valve to provide the required force. Simulation results are provided to show the effectiveness of the presented approach.

The wear failure course of gas-valve/valve-seat in engine was investigated with a simulating tester. The results show that the failure of the contact conical surface is mainly caused by the elastic and plastic deformation and the fatigue micro-crack and spalling. The creep-deformation and corrosion atmosphere accelerated wear failure course at the high temperature. The wear failure course of the gas-valve/valve-seat in engine follows general wear rules of mechanical elements, but the rate of wear in the sharp wear stage is faster.

Based on a theoretic hypothesis that income inequality has both positive and negative effect on economic growth, this paper analyzes the influence of income distribution differences on economic growth of China by establishing a multivariate linear regression model. The influence of income distribution difference on the economic growth of China changes with the change of time. During a short period immediately after China implemented reform and opening up policy, income inequality has a positive influence on the economic growth of China. However, along with the passing of time, such positive influence has become weaker and weaker and even convertes into negative effective. This paper proposes several advices with policy to restrain the difference of income distribution from expanding and weaken its negative effect on economic growth of China.

A new method was proposed to directly measure the effective resistance and distinguish the porous frequency in the regenerator of the microminiature thermoacoustic refrigerator. Measured results were compared with the flux gain factor and transmission loss of the real system. The results show that the agreement between the range of the porous frequency and frequency of the system is good, the method can be used to predict the porous frequency of the regenerator in production.

A numerical method was used to study the natural ventilation in a rectangular enclosure with two symmetrical openings. In order to improve the natural ventilation efficiency, a fin was introduced into the enclosure. Steady-state heat transfer by laminar natural ventilation in a partially divided rectangular enclosure was investigated by numerically solving equations of mass, momentum and energy. Streamlines and isotherms were produced and heat transfer rate were calculated. A parametric study was carried out using the following parameters: Rayleigh number (1×10³ − 1×10⁶), dimensionless length (0–0.7) and position values (−0.7−0.7). It is found that the Nusselt number is an increasing function of Rayleigh number. By comparing with no-fin case, it is concluded that fin can effectively enhance the natural ventilation in the enclosure.

Laminar natural convection in an enclosure divided by an adiabatic partition on its bottom with two unequal discrete heat sources was investigated numerically. The effect of the partition on the flow structure and heat transfer characteristics in enclosure under asymmetric heating condition was studied. The parameters are the Rayleigh number (1×10⁴≤Ra≤1×10⁶) and the height of partition (0≤h/H≤0.70). The streamlines are produced for various Rayleigh numbers. The results reflected by variations of the average Nusselt number in terms of the height of partition illustrates the convection heat transfer in the enclosure. The role of the partition is to weaken or cut off the heat removal rate from the strong heat source to the weak heat source. It is analyzed that the optimum height of the partition to break the linkage between the strong and weak heat source increases with increasing Rayleigh number.

A partition solution implemented by a cold air curtain for two asymmetric discrete heat sources in a two-dimensional rectangular enclosure was numerically studied. Main attentions were focused on the effects of Reynolds number, Grashof number, separation distance between heat sources, and buoyancy ratio. It is found that the airflow and heat transfer are not only determined by governing parameters, but also affected by boundary conditions. It is also found that nearly symmetry of flow structure corresponds to nearly thermal partition, and the symmetry can be enhanced when Reynolds number, separation distance and buoyancy ratio increase. In addition, it is observed that there is a minimum Reynolds number for obtaining nearly thermal partition, which increases when Grashof number increases.

In this paper, we apply a cone theoretic fixed point theorem to obtain sufficient conditions for the existence of multiple positive periodic solutions to the higher-dimensional functional difference equations of the form:

With the security problem of image information as the background, some more properties of the period of Arnold transformation of two-dimension were studied by means of introducing a integer sequence. Some new results are obtained. Two interesting conjectures on the period of Arnold transformation are given. When making digital images scrambling by Arnold transformation, it is important to know the period of the transformation for the image. As the application of the theory, a new method for computing the periods at last are proposed.

A multi-sequential number-theoretic optimization method based on clustering was developed and applied to the optimization of functions with many local extrema. Details of the procedure to generate the clusters and the sequential schedules were given. The algorithm was assessed by comparing its performance with generalized simulated annealing algorithm in a difficult instructive example and a D-optimum experimental design problem. It is shown the presented algorithm to be more effective and reliable based on the two examples.

By using the characteristic properties of the anti-Hermitian generalized anti-Hamiltonian matrices, we prove some necessary and sufficient conditions of the solvability for algebra inverse eigenvalue problem of anti-Hermitian generalized anti-Hamiltonian matrices, and obtain a general expression of the solution to this problem. By using the properties of the orthogonal projection matrix, we also obtain the expression of the solution to optimal approximate problem of an n×n complex matrix under spectral restriction.

The Filon-type quadrature is efficient for highly oscillatory functions — Fourier transforms. Based on Cox and Matthews’ ETD schemes, the higher order single step exponential time differencing schemes are presented based on the Filon-type integration and the A-stability of the two-order Adams-Bashforth exponential time differencing scheme is considered. The effectiveness and accuracy of the schemes is tested.

Objective To investigate the change of protein expression of lung tissue of rabbit after ischemic preconditioning (IP) and try to elucidate the potential protective mechanism of IP. Methods 12 domestic rabbits were randomly divided into group IP and group control (6 rabbits in each group). All the left lungs were afflicted by ische mia-reperfusion injury except that those in group IP were subject to IP prior to ischemic phase. 2-DE was employed to separate the total protein of the lung tissue. PDQuest analysis software was used to distinguish the differently expressed protein spot. MALDI-TOF-MS and Mascot database searching were exploited to identify these proteins. Results 1) IP attenuated the ischemia-reperfusion lung injury. 2) The proteomic analysis showed 35 target proteins, of which 17 were characterized such as phosphatidylinositol 3-kinase(PI3k) delta catalytic subunit. Conclusions 1) Proteomic is a promising tool to investigate the IP and ischemia-reperfusion lung injury. 2) That IP inhibits inflammatory cascades through phosphatidylinositol 3-kinase signal transduction pathway may be one of its protective mechanism.

Objective Tacrolimus is an immunosuppressive drug with narrow therapeutic range and wide interindividual variation in its pharmacokinetics. Tacrolimus is a substrate of cytochrome P450(CYP)3A5. The aim of this study was to evaluate whether the A6986G polymorphism is associated with tacrolimus concentration /dose ratio. Methods Fifty-two Chinese renal transplant patients were enrolled in this study. Their body weight, dosage and concentration of tacrolimus were observed. CYP3A5 genotype was determined by polymerase chain reaction followed by restriction fragment length polymorphism analysis. Results A significant association was found between tacrolimus levels per dose/kg/d and CYP3A5 gene A6986G polymorphism (P<0.001). The CYP3A5 * 3 * 3 patients have a significantly higher tacrolimus level/dose than CYP3A5 * 1 * 1 and CYP3A5 * 1 * 3. Conclusions CYP3A5 gene A6986G polymorphism is associated with tacrolimus pharmacokinetics and dose requirements. Pharmacogenetic methods could be employed prospectively to help the dose selection and to individualize immunosuppressive therapy according to the result.

Objective This pilot-study was designed to evaluate the feasibility of cell transplantation into guinea pig cochlea. Methods Marrow stromal cells were labeled with DAPI, and then implanted into the cochlea of guinea pig. The existence and differentiation trend were observed roughly two weeks later by histologic analysis. Results Transplant-derived marrow stem cells survived in cochlea two weeks later with a trend of attaching to cochlear architecture but not differentiate into neuron. Conclusions Transplant-derived marrow stem cells can survive in cochlea, and cell transplantation may be a useful strategy in inner ear diseases.

Objective To observe the apoptosis of osteosarcoma MG-63 cells induced by As₂O₃ and to explore its possible mechanisms. Methods The flowcytometric analysis and transmission electronmicroscope were performed to investigate the inducing apoptosis and inhibitative of As₂O₃ on osteosarcoma MG-63 cells. In order to study mechanism of apoptosis in MG-63 cells treated with As₂O₃, microarray was performed. The down-regulated gene was confirmed by RT-PCR, Northern-blotting. Results After treated with As₂O₃, hypodiploid peak before G0/G1 phase was observed in MG-63 cells through FCM analysis. Loss of microvilli, condensation and fragmentation of nuclear chromatin, condensation of cytoplasmic organelles, dilatation of the endoplasmic reticulum shrinkage of cells and alterations in cell membranes and apoptosis bodies which were observed in MG-63 cells treated with As₂O₃ by transmission electronmicroscope. The results of microarray show that As₂O₃ induced MG-63 cell apoptosis involves down-regulation of IEX-1 and the down-regulated gene is confirmed by RT-PCR and Northern-blotting. Conclusion The results show that As₂O₃ selectively inhibits growth of the solid tumor MG-63 cells by triggering apoptosis and indicates MG-63 induced by As₂O₃ cell apoptosis may through the IEX-1 pathway.

Objective Study on the effect of NF-κB in ox-LDL injured HUVEC-304 and the intervention with the serum of Tongmai Decoction. Methods Tongmai Decoction herbage-contained serum was made by the serum pharmacology methods, then the HUVEC-304 cells were divided into 5 groups and incubated for 24 h: 1) normal group; 2) fetal calf serum group; 3) ox-LDL group; 4) simvastatin group; 5) Tongmai Decoction group. RT-PCR was used to determine the mRNA of NF-κB in the cells, Western Blot Assay was used to determine the protein content of NF-κB in the cells. Results 1) Compared with the cells in normal group and serum control group, the endothelial cells in ox-LDL group are seriously injured, while those in Tongmai Decoction group grow better; 2) Compared with the cells in normal group and fetal calf serum group, the expressions of NF-κB gene and protein are up-regulated in ox-LDL group(P<0.05, P<0.01); 3) Compared with the cells in ox-LDL group, the expression of NF-κB gene and protein in Tongmai Decoction group are significantly down-regulated, there is no difference between Tongmai Decoction group and simvastatin group(PP>0.05). Conclusion Tongmai Decoction can protect the injured endothelial cells, up-regulate the expressions of NF-κB gene and protein content.

Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups, i. e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule, serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation, while all the plasma group presented platelet aggregation. P₁, P₅, P_max, t and T_M have no significant difference (P>0.05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin, P₁, P₅, t and P_max have significant differences (P<0.01) and T_M decreased significantly (P<0.05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P₁, t and P_max have significant difference (P<0.05), and P₅ and T_M are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P₁, P₅, t and P_max have significant differences (P<0.01), P₁, T_M have also significant (P<0.05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P₁, P₅ and P_max have significant differences (P<0.05), and t and P_max are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.

Objective To study on the role of thymus transplantation for heart allograft in rats. Methods Vascularized heart-thymus combined transplantation was performed with microsurgical technique. Graft survival, histopathology, level of IL-2, IL-4 and its mRNA expression in serum and cardiac grafts were investigated. Results Heart-thymus combined transplantation achieved effect in the prolongation of cardiac graft survival with short-term administration of cyclosporine. Conclusions Vascularized thymus transplantation induced immune tolerance in thymectomized rats.

HLCDG1, which locates in chromosome 5q33, is a novel gene cloned recently. The HLCDG1 expression was significantly down regulated in the primary lung carcinoma. It was previously studied that HLCDG1 acted like a tumor suppressor gene. In this paper, proteomics studies were performed to analyze the proteomic expression patterns in the HLCDG1-transfected human lung carcinoma cell line (A549-HLCDG1) and in the control vector-transfected human lung carcinoma cell line (A549-vector). Employing two dimensional gel electrophoresis (2DE), the global pattern of protein expressions in A549-HLCDG1 human lung adenocarcinoma cell line expressing stably HLCDG1 gene were compared with those of control A549-vector cell line to generate a differential protein expression catalog. Forty-two differentially expressed proteins were screened. Thirteen differential proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), which were 6 up-regulated (MSH5, MOD, MDH precursor, ETFβ, Prxd VI and JM23) and 7 downregulated (PLC-δ1, hnRNPA2, hnRNPB1, TIM, TCTP, nm23H-1 and PrxdV) proteins in A549-HLCDG1 cells compared to control A549-vector cells. The above identified proteins were involved in energy metabolism, transcription regulation, antioxidation, cell cycle, metastasis, DNA methylation and mismatch repair. Therefore, these differential expression proteins by HLCDG1 transfection may play some important roles for investigation of the biochemical basis of growth suppression of HLCDG1 gene in lung carcinoma cells A549. Further understanding of this data base may provide valuable resources for the developing novel diagnostic markers and therapeutic targets of lung cancer.

Objective To observe the effect of FTY720 and ICAM-1 mAb mono and combination therapy in cardiac allo-transplantation in rats. Methods Rats were randomly assigned to 9 groups, heart allo-transplantation were performed in abdominal site with micro-surgical technique. Recipients with allografts were treated with different doses of FTY720 and (or) ICAM-1 mAb. Graft survival, histopathology and level of serum IL-2, IFN-γ, IL-4, IL-10 were investigated. Results Low doses of FTY720 (1mg/kg) combined with ICAM-1 mAb achieved synergistic effect in the prolongation of cardiac graft survival, combination index(CI)=0.67. Conclusion Concomitant therapy of FTY720 and ICAM-1 mAb achieved a synergistic effect in the prolongation of heart allograft survival in rats.

Objective To establish and improve the model of heart-thymus composite transplantation. Methods Vascularized both lobes of the thymus is transplanted heterotopically with the heart as a composite graft in rats. This technique was developed and assessed, and viability of the grafts was evaluated histologically. Results Donor operation costed 38.5 ± 3.52 min, vascular anastomosis costed 25.0 ± 3.28 min, operating successful rate was 90%, acute rejection was observed in SD-Wistar group, viable thymus with normal microarchitecture was maintained in Wistar-Wistar group. Conclusions The improved novel technique for combined heart-thymus transplantation is a valuable method for study of the role of thymus in transplantation immunity.

Objective The purpose of this study is to identify the molecular basis of the contacting — neuron membrane K⁺ conductance in glia cells of native bovine retina. Methods RT-PCR, Northern blot and Western blot analyses were used to detect the expression of the inwardly rectifying K⁺ (Kir) channel subunits Kir2. 1 in native bovine RPE and neural retina. The distribution of Kir2. 1 protein was determined in frozen sections of bovine retina-RPE-choroid by indirect immunofluorescence analysis. Results RT-PCR analysis reveals Kir2. 1 transcript in both RPE and neural retina. In Northern blots, Kir2. 1 probe hybridizes to an appropriately sized-transcript in neural retina but not in RPE. In Western blots, Kir2. 1 antibody recognizes a major monomer of about 60 kDa in neural retina but not in RPE. Immunofluorescence reveals that Kir2. 1 immunostaining is expressed at many parts of Müller cells, especially in the membrane domains of Müller cells that contact retinal neurons, i. e., along the two stem processes, over the soma, and in the side branches extending into the synaptic layers. No immunostaining is seen in RPE. Doubling staining shows that Kir2. 1 proteins and glutamine synthetase proteins which are a marker of Müller cell co-localized well. Conclusions These results reveal that Kir2. 1 is localized in the Müller cells, no Kir2. 1 in RPE. These data suggests that Kir2. 1 may be involved in the transport of K⁺ in the bovine neural retina.

Objective To observe IL-1B, IL-6 and TNF-a level change of the joint fluid in the experimental knee joint degeneration and the effect of the local loosening therapy. Methods Thirty rabbits were divided into 3 groups at random: ten in the normal contrast group, ten in the blank model group and ten in the loosening therapy group to observe the IL-1B, IL-6 and TNF-a change of the joint fluid before and after the treatment in each group. Result IL-1B, IL-6 and TNF-a level in the blank model group was obviously higher than that in the normal group. (P<0.05). IL-1B, IL-6 and TNF-a level in the treatment group was obviously lower than that in the normal group. (P<0.05). Conclusions 1) IL-1B, IL-6 and TNF-a level of the joint fluid in the blank model group was obviously higher than that in the normal group. 2) Loosening soft tissues of the knees can improve IL-1B, IL-6 and TNF-a level of the joint fluid.

Background The pathogenesis of diabetic nephropathy (DN) is a complex pathophysiological process. Its precise mechanism is not fully known. In recent years it has been recognized that synthesis of various extracelluar matrix (ECM) components may increase, and that degradation of ECM may decrease in DN. It was reported heparin could inhibit mesangial cells proliferation in vitro. The main aim of this study is to explore whether heparin inhibits proliferation of mesangial cells grown in high glucose concentration and to measure the effect of heparin on matrix metalloproteinases (MMPs) expression in mesangial cells. Methods The medium contained either low glucose (5 mmol/L) or high glucose (25 mmol/L). The concentrations of heparin in the culture medium were 0, 25, 50, 100, 200 or 400 µg/mL. A metabolic (WST-1) assay was used to measure mesangial cell proliferation and Western blot analysis was used to measure MMPs expression of mesangial cells. Results Normal human mesangial cell (NHMC) proliferation was higher in high glucose (HG) medium than in low glucose (LG) medium. They showed a 1.93 fold expansion after 72 h in high glucose in contrast to a 1.63 fold expansion in low glucose. In the presence of heparin, mesangial cells proliferation was inhibited, which was more obvious at high glucose concentrations than at low glucose concentrations. In high glucose, with heparin concentration of 50, 100, 200 and 400 µg/mL, the mesangial cells showed a 0.61 fold, 0.52 fold, 0.52 fold and 0.41 fold reductions in cell number compared to cells grown without heparin. In low glucose, only concentrations of 200 µg/mL and 400 µg/mL showed reduction in cell number, namely 0.54 fold and 0.45 fold, when compared to cells grown without heparin. In Western blot analysis, MMP₁, MMP₂, MMP₃ and MMP₉ was expressed by mesangial cells expressed in both high and low glucose concentrations, which was more prominent in high glucose medium. Incubation of heparin further increased expression of MMP₁, MMP₂, MMP₃ and MMP₉. Conclusions This study suggests that glucose can accelerate mesangial cell proliferation while heparin can reduce proliferation, being more obvious at high glucose concentrations. Higher glucose concentrations led to increased MMP expression, which may take part in the regulation of mesangial matrix synthesis and degradation. Addition of heparin resulted in a corresponding increase in MMP expression, most notably at high glucose concentrations, indicating a potentially renoprotective role in DN.

Objective To assess stresses produced by different obturator prostheses. Methods Three-dimensional finite element models of unilateral maxillary defects rehabilitated with different obturators were constructed. The different stresses were analyzed by three-dimensional finite element method under different load angle. Results The Von Mises stress values obtained for the remaining tissues adjacent to defect cavity were higher when rehabilitated by inferior hollow obturator in comparison with by superior hollow obturator. The maximum of Von Mises were higher when rehabilitated by resilient hollow obturator than by rigid hollow obturator. It was also observed that in the rigid type stress distribution contours formed in the remaining tissues adjacent to defect cavity, while in resilient hollow obturator prostheses the stress distributed mainly in the prosthesis itself. The oblique load shows the most maximum of Von Mises among all types of obturator prostheses. Conclusions A high lateral wall of an obturator may be more better in preserving the remaining structures than a shorter prosthesis lateral wall. A soft liner may be incorporated to reduce the pain of the residual maxillary structures and mucosa. When load on defect, higher stress would be generated to the residual maxillary structures. The adjustment of occlusual relationship is very important.

Objective To investigate the effects of low dose Oxymatrine (OMT) on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff (CFDA-SE). Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT. Then, related software was used to analyze the effects of OMT on mouse lymphocyte proliferation. Results After cultured for 48 h, CFSE fluorescence could be detected by cytometer, filial generation peaks did not appear in control group, which indicated that lymphocytes did not proliferate. Three peaks were obviously detected in Con A group which indicated that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control group. In groups with Con A and OMT treated. Primary generation peaks are all lower while filial generation peaks are significantly higher than groups with Con A treated only. This indicated OMT obviously promote lymphocyte proliferation. After cultured for 72 h, the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h. Analyzed with CELLQuest software, it is shown that OMT could promote lymphocyte proliferation in 16, 8, 4 and 2 μg/mL respectively. Conclusions 1)CFDA-SE dyeing and flow cytometer were both reliable tools to analyse lymphocyte proliferation; 2) lower dosage of OMT could promote the proliferation of lymphocyte as a immunopotentiator.