•The significant development in gut microbiota has brought an unprecedented paradigm for pulmonary hypertension (PH) management in recent years, which has delighted researchers and clinicians in precise disease control. •The gut–lung axis is the bidirectional interaction between the gut and lung, which occurs through circulation, the nervous system, and other physiological processes, ultimately affecting the health of both the gut and the lung. •We provided a comprehensive landscape of gut microbiota and its associated metabolites in PH, emphasizing the interplay between microorganisms and hosts in modulating PH. •With the rapid development of technologies, including metagenomic and metabolomics on gut microbiota, the association of microorganisms and the human body will be untangled eventually, which will bring the dawn for disease cure followed by prognosis improvement among patients with PH.
•This study substantiates the potential clinical diagnostic utility of microbial composition and specific metabolites within seminal plasma for the asthenozoospermia (AZS) identification. •Divergent microbial compositions and metabolite profiles within seminal plasma are discernible across varying degrees of AZS severity. •Validation via in vitro and in vivo experiments corroborates hexadecanamide as a crucial metabolite influencing sperm motility in AZS, coupled with the dysregulation of target proteins (PAOX and CA2).
•Pregnancy complications critically affect maternal and child health, necessitating urgent research and therapeutic strategies to reduce health and socioeconomic impacts. •Gut microbiota dysbiosis in patients with various pregnancy complications acts as both a causal factor and a contributor to these conditions. •Gut microbiota-derived metabolites are involved in various pathophysiological pathways closely related to the pathogenesis of pregnancy complications, including intestinal barrier permeability, inflammatory responses, and glucose and lipid metabolism. •Emerging therapeutic strategies based on gut microbes show potential in treating pregnancy complications, yet there is a lack of evidence from randomized controlled trials (RCTs) to substantiate this approach.
•Deoxyribonucleic acid (DNA) data storage exhibits remarkable advantages, such as high density and long lifespan, and is suggested to be one of the most promising media for coping with future data storage crises. •A user-friendly platform, “Storage-D” was developed, which enables users to store any format of practical data with personalized choice. •Specifically, a new algorithm, named “Wukong” was developed, which contains a sizeable collection of codec pins that enables encoding data into any DNA sequence with considerable privacy. •The “Wukong” algorithm shows overall better performance than earlier algorithms in encoding a suitable length of DNA sequence matching downstream biochemical working flow for in vitro and in vivo storage. •The tool provides an open-frame for integrating other codec algorithms and can be easily connected to commercial DNA synthesis and sequencing platform for building a complete pipeline for practical data storage into DNA. The web server and codes of the platform are available at http://storage.dailab.xyz:16666/ and https://github.com/DNAstorage-iSynBio/Storage-D/, respectively.
•We first examined the natural assembly of resistome in healthy infants’ guts nd identified resistance genes that were significantly impacted by age. •We inferred that the age-dependent assembly of infant resistome was a result of the shift of the gut microbiome, which was due to resistance genes being disproportionally distributed across taxa. •Age-dependent assembly of infant resistome reflected the shift in the gut microbiome, and such modifications were often a result of dietary transitions during infancy. •Maturation of gut resistome was driven by infants’ changing carbohydrate metabolism, which demonstrated an increasing need for carbohydrateactive enzymes from Bacteroidota and decreasing involvements from Pseudomonadota during infancy.
•Symbiotic and pathogenic fungi are crucial in modulating various processes of cancer. •Dietary nutrients play essential physiological roles in growth, reproduction, and invasive pathogenesis of fungi. •The interactions between nutrients and fungi in cancer development provide valuable insights into the application of fungi-targeted strategies in precision nutrition for cancer prevention and treatment.
•Comprehensive genome analysis reveals underestimated species diversity and distinct functions of Lachnospiraceae. •Cataloging 1.5M genes highlights Lachnospiraceae’s significant role in the human gut microbiota, with potential novel species contributing. •Lachnospiraceae demonstrates a vast potential for synthesizing short-chain fatty acid (SCFA), producing secondary metabolites, and forming spores, which is promising for future studies and applications. •The abundance of Lachnospiraceae correlates inversely with diseases like atherosclerotic cardiovascular disease (ACVD) and inflammatory bowel disease (IBD), indicating a potential protective role.
•We identified the targets of artemisinin at different stages of the intraerythrocytic cycle of Plasmodium falciparum using a photoaffinity probe of artemisinin. •Artemisinin can interact with parasite proteins in vivo through both covalent and noncovalent mechanisms. •Artemisinin may exert its antimalarial effects by interfering with the parasite’s protein synthesis, glycolysis, and oxidative homeostasis pathways. •This study provides fresh insights into the mechanisms underlying artemisinin’s antimalarial effects and its protein targets.
•Sanghuangporus (SH) polyphenol extract potently alleviated symptoms of colon colitis in dextran sodium sulfate-induced colitis mice. •The gut microbiota especially Alistipes onderdonkii mediates the anticolitic effect of SH. •The SH-enriched microbial metabolite 5-hydroxyindole-3-acetic acid exhibited an anticolitic effect via the activation of the aryl hydrocarbon receptor. •The “gut microbiota-metabolites-signaling pathway” provides an effective perspective for the study of complex Chinese medicine systems.
•SpaC pilin acts as a causative factor of Lactobacillus rhamnosus GG-induced intestinal mucosa damage in zebrafish. •Dietary SpaC directly induces intestinal pyroptosis by activating GSDMEa in zebrafish. •Dietary SpaC induces gut microbial dysbiosis characterized by higher abundance of lipopolysaccharide (LPS)-producing gut microbes in zebrafish. •LPS-producing gut microbes subsequently activate Gaspy2−GSDMEb pyroptosis pathway in zebrafish.