Infant age inversely correlates with gut carriage of resistance genes, reflecting modifications in microbial carbohydrate metabolism during early life

Xinming Xu; Qingying Feng; Tao Zhang; Yunlong Gao; Qu Cheng; Wanqiu Zhang; Qinglong Wu; Ke Xu; Yucan Li; Nhu Nguyen; Diana H. Taft; David A. Mills; Danielle G. Lemay; Weiyun Zhu; Shengyong Mao; Anyun Zhang; Kelin Xu; Jinxin Liu

doi:10.1002/imt2.169

iMeta ›› 2024, Vol. 3 ›› Issue (2) : 169 DOI: 10.1002/imt2.169

RESEARCH ARTICLE

Infant age inversely correlates with gut carriage of resistance genes, reflecting modifications in microbial carbohydrate metabolism during early life

Xinming Xu ¹^,²^,³
, Qingying Feng ¹^,²^,⁴
, Tao Zhang ¹^,²
, Yunlong Gao ¹^,²
, Qu Cheng ⁵
, Wanqiu Zhang ¹^,²
, Qinglong Wu ⁶
, Ke Xu ⁷
, Yucan Li ⁸
, Nhu Nguyen ⁹
, Diana H. Taft ⁹
, David A. Mills ⁹^,¹⁰
, Danielle G. Lemay ¹¹
, Weiyun Zhu ¹^,²
, Shengyong Mao ¹^,²
, Anyun Zhang ¹²^,^†
, Kelin Xu ¹³^,^†
, Jinxin Liu ¹^,²^,^†

Author information +

¹. Laboratory of Gastrointestinal Microbiology, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China

². Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China

³. Department of Nutrition and Food Hygiene, School of Public Health, Institute of Nutrition, Fudan University, Shanghai, China

⁴. Biological Engineering Division, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA

⁵. Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

⁶. Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, USA

⁷. Department of Statistics, University of Chicago, Chicago, Illinois

⁸. State Key Laboratory of Genetic Engineering, Human Phenome Institute, Fudan University, Shanghai, China

⁹. Department of Food Science and Technology, University of California, Davis, Davis, California, USA

¹⁰. Department of Viticulture and Enology, Robert Mondavi Institute for Wine and Food Science, University of California, Davis, Davis, California, USA

¹¹. USDA ARS Western Human Nutrition Research Center, Davis, California, USA

¹². Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China

¹³. Department of Biostatistics, Key Laboratory of Public Health Safety, NHC Key Laboratory of Health Technology Assessment, School of Public Health, Fudan University, Shanghai, China

zhanganyu@scu.edu.cn

xukelin@fudan.edu.cn

jxnliu@njau.edu.cn

Show less

History +

PDF

Abstract

•We first examined the natural assembly of resistome in healthy infants’ guts nd identified resistance genes that were significantly impacted by age.

•We inferred that the age-dependent assembly of infant resistome was a result of the shift of the gut microbiome, which was due to resistance genes being disproportionally distributed across taxa.

•Age-dependent assembly of infant resistome reflected the shift in the gut microbiome, and such modifications were often a result of dietary transitions during infancy.

•Maturation of gut resistome was driven by infants’ changing carbohydrate metabolism, which demonstrated an increasing need for carbohydrateactive enzymes from Bacteroidota and decreasing involvements from Pseudomonadota during infancy.

Keywords

age / antimicrobial resistance genes / carbohydrate metabolism / diet / infant gut resistome / metagenomics

Cite this article

Download citation ▾

Xinming Xu, Qingying Feng, Tao Zhang, Yunlong Gao, Qu Cheng, Wanqiu Zhang, Qinglong Wu, Ke Xu, Yucan Li, Nhu Nguyen, Diana H. Taft, David A. Mills, Danielle G. Lemay, Weiyun Zhu, Shengyong Mao, Anyun Zhang, Kelin Xu, Jinxin Liu. Infant age inversely correlates with gut carriage of resistance genes, reflecting modifications in microbial carbohydrate metabolism during early life. iMeta, 2024, 3(2): 169 DOI:10.1002/imt2.169