Value of pain threshold of rats as index was determined by radiant heat-induced tail-flick to observe the effects of injection of TRH (10 μ/20 μ1) into cerebral ventricles (i.c.v.) on the analgesic effect induced by electro-acupuncture (1, 2 or 3 V) of bilateral “Zu San Li” and “San Yin Jiao”, and s.c. injection of morphine (2, 4, 6 mg/kg). The results of our study indicated that injection of TRH i.c.v. does not influence the radiant heat-induced tail-flick response of rats under normal condition, but antagonizes the analgesic effect caused by electro-acupuncture 2 and 3 V in strength and by s.c. infection of morphine (2, 4 mg/kg).
The effect of tetramethylpyrazine (TMPZ) on acute as well as chronic hypoxic pulmonary hypertension has been studied in rats. TMPZ inhibited acute hypoxic pulmonary vasoconstriction and prevented chronic hypoxic pulmonary hypertension and hypertrophy of the right ventricle. In normal rats and those with chronic pulmonary hypertension, TMPZ dramatically increased the cardiac output and decreased the pulmonary arterial pressure and pulmonary vascular resistance. More marked changes in pressure and resistance were observed in rats with chronic pulmonary hypertension than in normal rats. Therefore, it may be suggested that TMPZ proves of use in the prevention and treatment of acute and chronic hypoxic pulmonary hypertension and cor pulmonale.
Sera of patients infected withSchistosoma japonicum consistently reacted in immunoblots with 31 kD proteins fromSchistosoma mansoni worms. Control persons did not react. A human antiserum directed specifically against diagnostic 31 kD antigens of S.mansoni recognized components with the same molecular weight inS. japonicum. These cross-reacting 31 kD schistosome antigens may potentially be applied in a purified form for immunodiagnosis of schistosomiasis japonica.
From 91,725 autopsies, 28 cases of peripheral intrahepatic cholangiocarcinoma were selected to study the wide spectrum of histopathology of the tumor. The results showed that two cases (7.14 %) had predominantly solid type. One case had pure, and six had predominant cordai type (25%). Four showed pure, another four showed predominant ductal type (28.57%). Six (21.43%) revealed glandular pattern, one of which consisted of this pure component. Fapillary type was present in three cases (10.71 %) with two entirely made of this conformation. One case (3.57%) was classified as adenosquamous type and another one (3.57%) fell into the undifferentiated category. No obvious correlations between histological subtypes and underlying liver disease, stroma reaction or biological behaviour have been found. The significance of the classification in the tumor diagnosis and differential diagnosis, as well as possible mechanisms of carcinogenesis in these patients are discussed.
Presented in this paper is a new kind of splenic salvage operationvascularized heterotopic hemisplenic autotransplantation in dogs. All the experimental operations on 10 dogs met with success. The technique of surgery, operative complications and maintenance of splenic function of grafts are discussed. The authors believe that the operation is safe and clinically employable.
Eight kinds of urinary proteins were determined after renal transplantation. The results demonstrated that the concentrations of 8 urinary proteins were markedly increased when acute rejection occurred. Increase in urinary proteins was noticed 0–7 days prior to establishment of clinical diagnosis. Of the 8 urinary proteins IgG and transferrin appeared first. The positive rates of IgG and C3, both being 93 %, were the highest among them. Provided the antirejection treatment was effective, the concentration of urinary proteins would drop rapidly, otherwise it would remain at high levels.
It has been shown that gentamycin incorporated into polymethylmethacrylate (TJ bone cement or O-PMMA) can be released in the body. It can be continuously set free into the surroundings in therapeutic concentration in vivo for at least 10 months and inhibit several kinds of pathogenic bacteria for a prolonged period of time, e.g., Staphylococcus aureus for 300 days. It does not influence mechanical strength of TJ bone cement when 1 g of gentamycin powder is added to 20 g of cement. The present study offers a theoretical basis for the prevention of sepsis following artificial joint replacement and for the treatment of chronic osteomyelitis with domestic gentamycin-PMMA. A gentamycin-PMMA chain has been successfully made.
The distribution and excretion of intravenous injected3H-rhomotoxin was studied in mice. The radioactivity of3H-rhomotoxin was found to be high in gall bladder, liver and kidney, lower in thyroid gland, adrenal gland, heart and lungs, and lowest in brain. 52.4% of the drug was excreted in urine and feces within 6 h. Rhomotoxin was bound to plasma protein (60 % at 30 min) and to some extent to liver and kidney tissue.
Phenol mucilage was injected into the uterine cavities of rats to establish a model of endometrial adhesion, and then decoctions promoting blood circulation and dispersing blood stasis were given to them in order to prevent the formation of adhesion in one group and to treat it in another group. The results of the experiment showed that only a few rats suffered from extensive endometrial adhesion in the prevention group and there was only slight pathological change in the treatment group. This suggests that the prescription is useful in preventing and treating endometrial adhesions of rats induced by phenol mucilage, which provides a scientific basis for its clinical application to the treatment of intrauterine adhesions after curettage for induced abortion. The mechanism of action of the prescription in the prevention and treatment of endometrial adhesions is briefly discussed.
Four cases of Gorlin’s syndrome occurring in one family are described. Family memberI2 marriedI1; one of their daughters had the syndrome. The man married for the second time, and two of his children inherited the syndrome. The patients showed many of the characteristic features of the syndrome, such as jaw cysts, basal cell carcinoma, calcification of the falx cerebri, palmar or plantar dyskeratotic pits, congenital cataract, polydactyly, benign cysts and tumors. The symptom complex appeared to be inherited in an autosomal dominant pattern.
We have not found any numerical and structural abnormalities in cultured lymphocytes of the patients, but the SCE frequency was significantly higher in patients than in normal individuals. Recognition of this syndrome is extremely important, especially in children, because of the malignant potential and the need for seeking genetic consultation.
The slow-moving hemoglobin variant Hb Ottawa or α2 15(A13) Gly→Argβ2 was found in a heterozygote living in Hubei Province, People’s Republic of China. Structural characterization made use of high performance liquid chromatography (HPLC) and sequence analysis with the ultra micro-DABITC/PITC double coupling method.
Using immunoelectron microscopy to observe the distribution of substance P-positive nerve terminals in the superior cervical ganglion of the rat, the authors found that the substance P-containing nerve terminals appear in the neuropil or around the perikarya of the principal neurons and form symmetrical axo-somatic synapses with the body of the principal neurons. Morphological features described suggest that substance P might play a certain role in regulating sympathetic function.
A model of acute farmer’s lung (FL) was successfully developed with Thermoactinomyces vulgaris isolated from hay in the region where a patient with FL was living. 117 rabbits were divided into 5 groups, which were given 1 or 3 intratracheal injections of 50 mg or 5 mg spores suspension respectively. After the last injection the animals were sacrificed at different intervals and the lungs were removed. Specimens were submitted to routine histologic examination and also to immunofluorescent and ultrastructural studies. Morphologic studies revealed that the intratracheal inoculation of rabbits with the spores produced a mononuclear interstitial pneumonitis similar to that seen in man. Electron microscopy showed spores in the cytoplasm of phagocytes. Immunofluorescent examination revealed deposition of IgG in the pulmonary lesions. The present study confirms that the pulmonary lesions are reversible in acute stage and also suggests that the spores not only have a direct pathogenetic effect but also can give rise to typeI and type IV hypersensitivity reactions in inducing FL.