Frontiers of Medicine >

2009 , Vol. 3 >Issue 1: 49 - 56

DOI: https://doi.org/10.1007/s11684-009-0012-y

RESEARCH ARTICLE

Gene expression disparity in giant cell tumor of bone

  • Xiaohua PAN 1,2 ,
  • Shuhua YANG , 1 ,
  • Deming XIAO 2 ,
  • Yong DAI 3 ,
  • Lili REN 3
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  • 1. Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • 2. Department of Orthopaedics, Shenzhen People’s Hospital, The Second Medical College of Jinan University, Shenzhen 518020, China
  • 3. Clinical Research Center, Shenzhen People’s Hospital, The Second Hospital Medical College of Jinan University, Shenzhen 518020, China

Received date: 21 Oct 2008

Accepted date: 15 Nov 2008

Published date: 05 Mar 2009

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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Abstract

The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology. Total RNA of 8 fresh GCTB specimens (Jaffe I∶6 cases, II∶1 case, III∶1 case; Campanacci I∶6 cases, II∶1 case, III∶1 case; Enneking Staging G0T1-2M0: 5 cases, G1T1-2M0: 2 cases, G1T2M0: 1 case) and 4 normal bony callus specimens (the control group) were extracted and purified to get mRNA and then reverse transcribed to complementary DNA, respectively. Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control. The hybridization signals were scanned. The gene expression disparity between the GCTB samples and normal bony callus was significantly different (P<0.01), and the disparity of over 5-fold was found in 47 genes in the GCTB specimens, with 25 genes up-regulated and 22 down-regulated including the extracellular matrix and transforming-related genes, oncogene and its homolog genes, cytokine and its receptor genes. Specific gene spectrum associated with GCTB can be identified by cDNA microarray, which will be the foundation of progressive etiology elucidation, diagnosis and treatment of GCTB.

Key words： giant cell tumor of bone; gene; microarray, cDNA

Cite this article

Xiaohua PAN , Shuhua YANG , Deming XIAO , Yong DAI , Lili REN . Gene expression disparity in giant cell tumor of bone[J]. Frontiers of Medicine, 2009 , 3(1) : 49 -56 . DOI: 10.1007/s11684-009-0012-y

Acknowledgements

This project was supported by a grant from the Shenzhen Municipal Research Program of Sciences and Technology (No. 200204061).

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