Gene expression disparity in giant cell tumor of bone

Abstract

The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology. Total RNA of 8 fresh GCTB specimens (Jaffe I∶6 cases, II∶1 case, III∶1 case; Campanacci I∶6 cases, II∶1 case, III∶1 case; Enneking Staging G₀T_1-2M₀: 5 cases, G₁T_1-2M₀: 2 cases, G₁T₂M₀: 1 case) and 4 normal bony callus specimens (the control group) were extracted and purified to get mRNA and then reverse transcribed to complementary DNA, respectively. Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control. The hybridization signals were scanned. The gene expression disparity between the GCTB samples and normal bony callus was significantly different (P<0.01), and the disparity of over 5-fold was found in 47 genes in the GCTB specimens, with 25 genes up-regulated and 22 down-regulated including the extracellular matrix and transforming-related genes, oncogene and its homolog genes, cytokine and its receptor genes. Specific gene spectrum associated with GCTB can be identified by cDNA microarray, which will be the foundation of progressive etiology elucidation, diagnosis and treatment of GCTB.

Keywords

giant cell tumor of bone / gene / microarray, cDNA

References

[1] TubbsW S,BrownL R,BeaboutJ W,RockM G,UnniK K.Benign giant-cell tumor of bone with pulmonary metastases: clinical findings and radiologic appearance of metastases in 13 cases.AJR,1992,158(3): 331-334

[2] SungH W,KuoD P,ShuW P,ChaiY B,LiuC C,LiS M.Giant-cell tumor of bone: analysis of two hundred and eight cases in Chinese patients.J Bone Joint Surg Am,1982, 64(5):755-761

[3] ProsserG H,BalochK G,TillmanR M,CarterS R,GrimerR J.Does curettage without adjuvant therapy provide low recurrence rates in giant-cell tumors of bone?Clin Orthop Relat Res,2005,435(6):211-218

[4] LadanyiM,TraganoF,HuvosA,Benign metastasizing giant cell tumours of bone. Cancer,1989,64(7):1521-1526

[5] TianB L,WenJ M,ZhangM,XieD,XuR B,LuoC J.The expression of ADAM12 (meltrin alpha) in human giant cell tumours of bone.Mol Pathol,2002,55(6):394-397

[6] KumtaS M,HuangL,ChengY Y,ChowL T C,LeeK M,ZhengM H.Expression of VEGF and MMP-9 in giant cell tumor of bone and other osteolytic lesions.Life Sci, 2003, 73(11): 1427-1436

[7] WelfordS M,GreggJ,ChenE,GarrisonD,SorensenP H,DennyC T,NelsonS F. Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization.Nucleic Acids Res,1998,26(12): 3059-3065

[8] LiD P,WangK,DaiY,LVT Y.Gene Expression Profiling on Acute Rejected Transplant Kidneys with Microarray.J Huazhong Univ Sci Technol (Med Sci).2008,28(2): 136-139

[9] LogarD B,KomadinaR,PrezeljJ,OstanekB,TrostZ,MarcJ.Expression of bone resorption genes in osteoarthritis and in osteoporosis.J Bone Miner Metab,2007,25(4): 219-225

[10] LaceyD L,TimmsE,TanH L,KelleyM J,DunstanC R,BurgessT,ElliottR, ColomberoA,ElliottG,ScullyS,HsuH,SullivanJ,HawkinsN,DavyE,CapparelliC,EliA, QianY X,KaufmanS,SarosiI,ShalhoubV,SenaldiG,GuoJ,DelaneyJ,BoyleW J. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell,1998,93(2):165-176

[11] KongY Y,FeigeU,SarosiI,BolonB,TafuriA,MoronyS,CapparelliC,LiJ,ElliottR, McCabeS,WongT,CampagnuoloG,MoranE,BogochE R,VanG,NguyenL T,OhashiP S,LaceyD L,FishE,BoyleW J,PenningerJ M.Activated T cells regulate bone loss and joint destuction in adjuvant arthritis through osteoprotegerin ligand.Nature,1999, 402(6759):304-309

[12] TakayanagiH,KimS,MatsuoK,SuzukiH,SuzukiT,SatoK,YokochiT,OdaH, NakamuraK,IdaN,WagnerE F,TaniguchiT.RANKL maintaims bone homeostasis through c-Fos-dependent induction of interferon-beta.Nature,2002,418(6882):744-749

[13] GuentherR,KrennV,MorawietzL,DankofA,MelcherI,SchaserK D,KasperH U, KubanR J,UngethümU,SersC.Giant cell tumors of the bone: molecular profiling and expression analysis of Ephrin A1 receptor, Claudin 7, CD52, FGFR3 and AMFR.Pathol Res Pract,2005,201(10):649-663

[14] SaizP,VirkusW,PiaseckiP,TempletonA,ShottS,GitelisS.Results of giant cell tumor of bone treated with intralesional excision.Clin Orthop,2004, 7(424): 221-226

[15] LackmanR D,HosalkarH S,OgilvieC M,TorbertJ T,FoxE J.Intralesional curettage for grades II and III giant cell tumors of bone.Clin Orthop Relat Res,2005, 438(9): 123-127

[16] LaustenG S,JensenP K,SchiødtT,LundB.Local recurrences in giant cell tumour of bone: long-term follow up of 31 cases.Int Orthop,1996,20(3):172-176

[17] GhertM A,RizzoM,HarrelsonJ M,ScullyS P.Giant cell tumor in the appendicular skeleton,Clin Orthop Relat Res, 2002, 400(7): 201-210

Acknowledgements

This project was supported by a grant from the Shenzhen Municipal Research Program of Sciences and Technology (No. 200204061).

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg