Stress response to nanoplastics with different charges in Brassica napus L. during seed germination and seedling growth stages

Highlights

● Higher concentrations of PS, PS-NH₂ and PS-SO₃H inhibited seed germination.

● PS, PS-NH₂ and PS-SO₃H influenced seedling growth in a dose-dependent manner.

● PS, PS-NH₂ and PS-SO₃H reduced essential nutrients uptake and plant quality.

● PS, PS-NH₂ and PS-SO₃H increased antioxidant enzyme activities and MDA content.

● Nanoplastic toxicity was related to surface charges.

Abstract

Nanoplastic pollution has become a significant problem in farmland systems worldwide. However, research on the effects of nanoplastics (NPs) with different charges on field crops is still limited. In our study, NPs with different charges, including unmodified polystyrene nanoplastics (PS), positively charged polystyrene nanoplastics (PS-NH₂), and negatively charged polystyrene nanoplastics (PS-SO₃H), were investigated for their impacts on seed germination and seedling growth of rape. The results showed that seed water uptake (after 12 h), seed germination, seed vigour, and relative root elongation were all significantly reduced under exposure to NPs (200 mg/L). Similarly, remarkable decreases in plant biomass (root weight, shoot weight), growth (root length, plant height), photosynthesis ability (chlorophyll a, chlorophyll b, carotenoids), essential nutrient uptake (Fe, Mn, Zn, Cu), and plant quality (soluble protein, soluble sugar, crude fibre content) of rape seedlings were also observed after exposure to NPs. Among the three kinds of NPs, PS-NH₂ showed stronger effects. Moreover, superoxide dismutase, peroxidase, and catalase activities of rape seedlings were changed, and the content of malondialdehyde was significantly increased under exposure to NPs. Furthermore, positively charged PS-NH₂ showed stronger effects on the phenotype, physiology, biochemistry, nutrient uptake, and plant quality of rape. Notably, a comprehensive toxicity evaluation revealed that PS-NH₂ had the strongest toxicity to rape. The present study provides important implications for the interaction and risk assessment of NPs and crops in soil-plant systems.

Graphical abstract

Keywords

Nanoplastics / Rape (Brassica napus L.) / Physiology and biochemistry / Nutrient absorption / Plant quality / Toxicity

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Nos. U20A20115 and 42007112) and the Major Technology Innovation Project of Shandong Province (No. 2020CXGC011403). The authors would like to thank all the anonymous referees for their constructive comments and suggestions.

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-023-1643-y and is accessible for authorized users.

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