Effects of Al3+ on pollutant removal and extracellular polymeric substances (EPS) under anaerobic, anoxic and oxic conditions
Lanhe Zhang, Jing Zheng, Jingbo Guo, Xiaohui Guan, Suiyi Zhu, Yanping Jia, Jian Zhang, Xiaoyu Zhang, Haifeng Zhang
Effects of Al3+ on pollutant removal and extracellular polymeric substances (EPS) under anaerobic, anoxic and oxic conditions
The highest removal efficiencies of COD and TN were achieved under 10 mg/L of Al3+.
The highest TP removal efficiency occurred under 30 mg/L of Al3+.
EPS, PS and PN concentrations increased with the addition of Al3+.
Sludge properties significantly changed with the addition of Al3+.
Aluminum ions produced by aluminum mining, electrolytic industry and aluminum-based coagulants can enter wastewater treatment plants and interact with activated sludge. They can subsequently contribute to the removal of suspended solids and affect activated sludge flocculation, as well as nitrogen and phosphorus removal. In this study, the effects of Al3+ on pollutant removal, sludge flocculation and the composition and structure of extracellular polymeric substances (EPS) were investigated under anaerobic, anoxic and oxic conditions. Results demonstrated that the highest chemical oxygen demand (COD) and total nitrogen (TN) removal efficiencies were detected for an Al3+ concentration of 10 mg/L. In addition, the maximal dehydrogenase activity and sludge flocculation were also observed at this level of Al3+. The highest removal efficiency of total phosphorus (TP) was achieved at an Al3+ concentration of 30 mg/L. The flocculability of sludge in the anoxic zone was consistently higher than that in the anaerobic and oxic zones. The addition of Al3+ promoted the secretion of EPS. Tryptophan-like fluorescence peaks were detected in each EPS layer in the absence of Al3+. At the Al3+ concentration of 10 mg/L, fulvic acid and tryptophan fluorescence peaks began to appear, while the majority of protein species and the highest microbial activity were also detected. Low Al3+ concentrations (<10 mg/L) could promote the removal efficiencies of COD and TN, yet excessive Al3+ levels (>10 mg/L) weakened microbial activity. Higher Al3+ concentrations (>30 mg/L) also inhibited the release of phosphorus in the anaerobic zone by reacting with PO43-.
Extracellular polymeric substances / Activated sludge / Aluminum ion / A2O / Wastewater
[1] |
Agridiotis V, Forster C F, Carliell-Marquet C (2007). Addition of Al and Fe salts during treatment of paper mill effluents to improve activated sludge settlement characteristics. Bioresource Technology, 98(15): 2926–2934
CrossRef
Pubmed
Google scholar
|
[2] |
Baker A, Curry M (2004). Fluorescence of leachates from three contrasting landfills. Water Research, 38(10): 2605–2613
CrossRef
Pubmed
Google scholar
|
[3] |
Chen W, Westerhoff P, Leenheer J A, Booksh K (2003). Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environmental Science & Technology, 37(24): 5701–5710
CrossRef
Pubmed
Google scholar
|
[4] |
Frolund B, Griebe T, Nielsen P H (1995). Enzymatic activity in the activated-sludge floc matrix. Applied Microbiology and Biotechnology, 43(4): 755–761
CrossRef
Pubmed
Google scholar
|
[5] |
Ge J, Meng X, Song Y, Terracciano A (2018). Effect of phosphate releasing in activated sludge on phosphorus removal from municipal wastewater. Journal of Environmental Sciences-China, 67(5): 216–223
CrossRef
Pubmed
Google scholar
|
[6] |
Hudson N, Baker A, Ward D, Reynolds D M, Brunsdon C, Carliell-Marquet C, Browning S (2008). Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England. Science of the Total Environment, 391(1): 149–158
CrossRef
Pubmed
Google scholar
|
[7] |
Hwang K L, Bang C H, Zoh K D (2016). Characteristics of methane and nitrous oxide emissions from the wastewater treatment plant. Bioresource Technology, 21: 881–884
CrossRef
Pubmed
Google scholar
|
[8] |
Ji B, Yang K, Wang H (2015). Impacts of poly-aluminum chloride addition on activated sludge and the treatment efficiency of SBR. Desalination and Water Treatment, 54(9): 2376–2381
CrossRef
Google scholar
|
[9] |
Li A, Zhou C, Liu Z L, Xu X Y, Zhou Y, Zhou D D, Tang Y N, Ma F, Rittmann B E (2018). Direct solid-state evidence of H2-induced partial U(VI) reduction concomitant with adsorption by extracellular polymeric substances (EPS). Biotechnology and Bioengineering, 115(7): 1685–1693
CrossRef
Pubmed
Google scholar
|
[10] |
Li H J, Taniguchi Y (2019). Load-carrying capacity of semi-rigid double-layer grid structures with initial crookedness of member. Engineering Structures, 184(1): 421–433
CrossRef
Google scholar
|
[11] |
Li M, Chen Y G, Su Y L, Wan R, Zheng X (2016). Effect of fulvic acids with different characteristics on biological denitrification. RSC Advances, 6(18): 14993–15001
CrossRef
Google scholar
|
[12] |
Li X Y, Yang S F (2007). Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge. Water Research, 41(5): 1022–1030
CrossRef
Pubmed
Google scholar
|
[13] |
Lin J X, Jiang X, Cao J S, Fang F, Feng Q (2018). Influences of phosphorous removal chemicals on EBPR system in wastewater treatment. Water Purification Technology, 37(12): 84–90 (in Chinese)
|
[14] |
Liu Y, Liu Z, Wang F, Chen Y, Kuschk P, Wang X (2014). Regulation of aerobic granular sludge reformulation after granular sludge broken: Effect of poly aluminum chloride (PAC). Bioresource Technology, 158: 201–208
CrossRef
Pubmed
Google scholar
|
[15] |
Ma B, Chen G, Hu C, Liu Z, Liu H, Qu J (2018). Speciation matching mechanisms between orthophosphate and aluminum species during advanced P removal process. Science of the Total Environment, 642(15): 1311–1319
CrossRef
Pubmed
Google scholar
|
[16] |
Ma W C, Zhao L, Liu H L, Liu Q L, Ma J (2017). Improvement of sludge dewaterability with modified cinder via affecting EPS. Frontiers of Environmental Science and Engineering, 11(6): 19
|
[17] |
Mehrnia M R, Azami H, Sarrafzadeh M H (2013). Fouling mitigation in membrane bioreactors using multivalent cations. Colloids and Surfaces. B, Biointerfaces, 109: 90–96
CrossRef
Pubmed
Google scholar
|
[18] |
Nie Y L, Tian X K, Zhou Z X, Li Y Y (2017). Impact of food to microorganism ratio and alcohol ethoxylate dosage on methane production in treatment of low-strength wastewater by a submerged anaerobic membrane bioreactor. Frontiers of Environmental Science and Engineering, 11(6): 6
|
[19] |
Park C, Fang Y, Murthy S N, Novak J T (2010). Effects of floc aluminum on activated sludge characteristics and removal of 17-a-ethinylestradiol in wastewater systems. Water Research, 44(5): 1335–1340
CrossRef
Pubmed
Google scholar
|
[20] |
Pino-Jelcic S A, Hong S M, Park J K (2006). Enhanced anaerobic biodegradability and inactivation of fecal coliforms and Salmonella spp. in wastewater sludge by using microwaves. Water Environment Research, 78(2): 209–216
CrossRef
Pubmed
Google scholar
|
[21] |
Ruan X, Li L, Liu J (2013). Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances. Journal of Environmental Sciences-China, 25(5): 916–924
CrossRef
Pubmed
Google scholar
|
[22] |
Salama Y, Chennaoui M, Sylla A, Mountadar M, Rihani M, Assobhei O (2016). Characterization, structure, and function of extracellular polymeric substances (EPS) of microbial biofilm in biological wastewater treatment systems: A review. Desalination and Water Treatment, 57(35): 16220–16237
CrossRef
Google scholar
|
[23] |
Sheng G P, Yu H Q, Li X Y (2010). Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: A review. Biotechnology Advances, 28(6): 882–894
CrossRef
Pubmed
Google scholar
|
[24] |
Sheng G P, Yu H Q, Yue Z B (2005). Production of extracellular polymeric substances from Rhodopseudomonas acidophila in the presence of toxic substances. Applied Microbiology and Biotechnology, 69(2): 216–222
CrossRef
Pubmed
Google scholar
|
[25] |
Sondhi A, Guha S, Harendranath C S, Singh A (2010). Effect of aluminum (Al3+) on granulation in upflow anaerobic sludge blanket reactor treating low-strength synthetic wastewater. Water Environment Research, 82(8): 715–724
CrossRef
Pubmed
Google scholar
|
[26] |
Sponza D T (2003). Investigation of extracellular polymer substances (EPS) and physicochemical properties of different activated sludge flocs under steady-state conditions. Enzyme and Microbial Technology, 32(3–4): 375–385
CrossRef
Google scholar
|
[27] |
Sun M, Yan L, Zhang L, Song L, Guo J, Zhang H (2019). New insights into the rapid formation of initial membrane fouling after in-situ cleaning in a membrane bioreactor. Process Biochemistry, 78: 108–113
CrossRef
Google scholar
|
[28] |
Tian M, Zhao F, Shen X, Chu K, Wang J, Chen S, Guo Y, Liu H (2015). The first metagenome of activated sludge from full-scale anaerobic/anoxic/oxic (A2O) nitrogen and phosphorus removal reactor using Illumina sequencing. Journal of Environmental Sciences-China, 35(1): 181–190
CrossRef
Pubmed
Google scholar
|
[29] |
Wang C, He R, Wu Y, Lürling M, Cai H, Jiang H L, Liu X (2017). Bioavailable phosphorus (P) reduction is less than mobile P immobilization in lake sediment for eutrophication control by inactivating agents. Water Research, 109(1): 196–206
CrossRef
Pubmed
Google scholar
|
[30] |
Wang S Y, He Y L, Li X Y, Jia F X, Guo S Y (2016). Optimization of extracellular polymeric substance extraction method of different sludge. Journal of Beijing University of Technology, 42(4): 569–576 (in Chinese)
|
[31] |
Wilén B M, Jin B, Lant P (2003). The influence of key chemical constituents in activated sludge on surface and flocculating properties. Water Research, 37(9): 2127–2139
CrossRef
Pubmed
Google scholar
|
[32] |
Yang T, Hao X K, Chen B Y, Chen Y X, Zhang Y, Yang K (2018). Effects of Al3+ on dehydrogenase activity (DHA) and extracellular polymeric substances (EPS) of activated sludge in a sequencing batch biofilm reactor (SBBR). Acta Scientiae Circumstantiae, 38(4): 1453–1459 (in Chinese)
|
[33] |
Zhang H F, Sun M, Song L F, Guo J B, Zhang L H (2019a). Fate of NaClO and membrane foulants during in-situ cleaning of membrane bioreactors: Combined effect on thermodynamic properties of sludge. Biochemical Engineering Journal, 147: 146–152
CrossRef
Google scholar
|
[34] |
Zhang L H, Zhang M S, Guo J B, Zheng J, Chen Z C, Zhang H F (2019b). Effects of K+ salinity on the sludge activity and the microbial community structure of an A2O process. Chemosphere, 235: 805–813
CrossRef
Pubmed
Google scholar
|
/
〈 | 〉 |