Frontiers of Chemical Science and Engineering >

2007 , Vol. 1 >Issue 4: 399 - 403

DOI: https://doi.org/10.1007/s11705-007-0073-x

High-expression of recombinant human concensus interferon-α by Pichia pastoris

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  • 1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.Institute of Bioengineering, Academy of Military Medical Sciences, Beijing 100071, China

Published date: 05 Dec 2007

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Abstract

The present work focused on the high expression of recombinant human consensus interferon-α (cIFN) by Pichia pastoris. The cycle of glycerol feeding, the strategy of methanol feeding and the optimum pH for protein induction were studied. The optimized strategies were a 4-h glycerol-feeding period, induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L. The maximum dry cell weight, cIFN production and bioactivity obtained were 168, 1.24 g/L and 5.4×107 U/mL, respectively.

Cite this article

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin . High-expression of recombinant human concensus interferon-α by Pichia pastoris[J]. Frontiers of Chemical Science and Engineering, 2007 , 1(4) : 399 -403 . DOI: 10.1007/s11705-007-0073-x

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