High-expression of recombinant human concensus interferon-α by Pichia pastoris

Abstract

The present work focused on the high expression of recombinant human consensus interferon-α (cIFN) by Pichia pastoris. The cycle of glycerol feeding, the strategy of methanol feeding and the optimum pH for protein induction were studied. The optimized strategies were a 4-h glycerol-feeding period, induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L. The maximum dry cell weight, cIFN production and bioactivity obtained were 168, 1.24 g/L and 5.4×10⁷ U/mL, respectively.

Keywords

consensus interferon-α, high-expression, Pichia pastoris, glycerol feeding, methanol feeding