High-expression of recombinant human concensus interferon-α by Pichia pastoris

doi:10.1007/s11705-007-0073-x

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Front. Chem. Sci. Eng. ›› 2007, Vol. 1 ›› Issue (4) : 399-403. DOI: 10.1007/s11705-007-0073-x

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou¹, SHI Qiqi¹, HE Yun¹, ZHUANG Yingping¹, WANG Yonghong¹, ZHANG Siliang¹, CHU Ju¹, LIU Zhimin²

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Abstract

The present work focused on the high expression of recombinant human consensus interferon-α (cIFN) by Pichia pastoris. The cycle of glycerol feeding, the strategy of methanol feeding and the optimum pH for protein induction were studied. The optimized strategies were a 4-h glycerol-feeding period, induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L. The maximum dry cell weight, cIFN production and bioactivity obtained were 168, 1.24 g/L and 5.4×10⁷ U/mL, respectively.

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HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin. High-expression of recombinant human concensus interferon-α by Pichia pastoris. Front. Chem. Sci. Eng., 2007, 1(4): 399‒403 https://doi.org/10.1007/s11705-007-0073-x