Frontiers of Chemical Science and Engineering >
Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain
Received date: 26 Sep 2011
Accepted date: 12 Jan 2012
Published date: 05 Jun 2012
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Evolutionary engineering is a novel whole-genome wide engineering strategy inspired by natural evolution for strain improvement. Astaxanthin has been widely used in cosmetics, pharmaceutical and health care food due to its capability of quenching active oxygen. Strain improvement of Phaffia rhodozyma, one of the main sources for natural astaxanthin, is of commercial interest for astaxanthin production. In this study a selection procedure was developed for adaptive evolution of P. rhodozyma strains under endogenetic selective pressure induced by additive in environmental niches. Six agents, which can induce active oxygen in cells, were added to the culture medium respectively to produce selective pressure in process of evolution. The initial strain, P. rhodozyma AS2-1557, was mutagenized to acquire the initial strain population, which was then cultivated for 550 h at selective pressure and the culture was transferred every 48h. Finally, six evolved strains were selected after 150 generations of evolution. The evolved strains produced up to 48.2% more astaxanthin than the initial strain. Our procedure may provide a promising alternative for improvement of high-production strain.
Key words： evolutionary engineering; astaxanthin; strain improvement
Jixian GONG , Nan DUAN , Xueming ZHAO . Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain[J]. Frontiers of Chemical Science and Engineering, 2012 , 6(2) : 174 -178 . DOI: 10.1007/s11705-012-1276-3
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