Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain

Jixian GONG, Nan DUAN, Xueming ZHAO

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PDF(402 KB)
Front. Chem. Sci. Eng. ›› 2012, Vol. 6 ›› Issue (2) : 174-178. DOI: 10.1007/s11705-012-1276-3
RESEARCH ARTICLE

Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain

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Abstract

Evolutionary engineering is a novel whole-genome wide engineering strategy inspired by natural evolution for strain improvement. Astaxanthin has been widely used in cosmetics, pharmaceutical and health care food due to its capability of quenching active oxygen. Strain improvement of Phaffia rhodozyma, one of the main sources for natural astaxanthin, is of commercial interest for astaxanthin production. In this study a selection procedure was developed for adaptive evolution of P. rhodozyma strains under endogenetic selective pressure induced by additive in environmental niches. Six agents, which can induce active oxygen in cells, were added to the culture medium respectively to produce selective pressure in process of evolution. The initial strain, P. rhodozyma AS2-1557, was mutagenized to acquire the initial strain population, which was then cultivated for 550 h at selective pressure and the culture was transferred every 48h. Finally, six evolved strains were selected after 150 generations of evolution. The evolved strains produced up to 48.2% more astaxanthin than the initial strain. Our procedure may provide a promising alternative for improvement of high-production strain.

Keywords

evolutionary engineering / astaxanthin / strain improvement

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Jixian GONG, Nan DUAN, Xueming ZHAO. Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain. Front Chem Sci Eng, 2012, 6(2): 174‒178 https://doi.org/10.1007/s11705-012-1276-3

References

[1]
Selifonova O, Valle F, Schellenberger V. Rapid evolution of novel traits in microorganisms. Applied and Environmental Microbiology, 2001, 67(8): 3645-3649
CrossRef Google scholar
[2]
Sonderegger M, Sauer U. Evolutionary engineering of Saccharomyces cerevisiae for anaerobic growth on xylose. Applied and Environmental Microbiology, 2003, 69(4): 1990-1998
CrossRef Google scholar
[3]
Steiner P, Sauer U. Long-term continuous evolution of acetate resistant Acetobacter aceti. Biotechnology and Bioengineering, 2003, 84(1): 40-44
CrossRef Google scholar
[4]
van Maris A J A, Geertman J M A, Vermeulen A, Groothuizen M K, Winkler A A, Piper M D W, van Dijken J P, Pronk J T. Directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, yielding a C-2-independent, glucose-tolerant, and pyruvate-hyperproducing yeast. Applied and Environmental Microbiology, 2004, 70(1): 159-166
CrossRef Google scholar
[5]
Cakar Z P, Seker U O S, Tamerler C, Sonderegger M, Sauer U. Evolutionary engineering of multiple-stress resistant Saccharomyces cerevisiae. FEMS Yeast Research, 2005, 5(6-7): 569-578
CrossRef Google scholar
[6]
Kuyper M, Toirkens M, Diderich J, Winkler A, Vandijken J, Pronk J. Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain. FEMS Yeast Research, 2005, 5: 925-934
CrossRef Google scholar
[7]
Wisselink H W, Toirkens M J, del Rosario Franco Berriel M, Winkler A A, van Dijken J P, Pronk J T, van Maris A J A. Engineering of Saccharomyces cerevisiae for efficient anaerobic alcoholic fermentation of L-arabinose. Applied and Environmental Microbiology, 2007, 73(15): 4881-4891
CrossRef Google scholar
[8]
Guimaraes P M R, Francois J, Parrou J L, Teixeira J A, Domingues L. Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant. Applied and Environmental Microbiology, 2008, 74(6): 1748-1756
CrossRef Google scholar
[9]
Jantama K, Haupt M J, Svoronos S A, Zhang X, Moore J C, Shanmugam K T, Ingram L O. Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate. Biotechnology and Bioengineering, 2008, 99(5): 1140-1153
CrossRef Google scholar
[10]
Meijnen J P, de Winde J H, Ruijssenaars H J. Engineering pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose. Applied and Environmental Microbiology, 2008, 74(16): 5031-5037
CrossRef Google scholar
[11]
Cakar Z P, Alkım C, Turanlı B, Tokman N, Akman S, Sarıkaya M, Tamerler C, Benbadis L, François J M. Isolation of cobalt hyper-resistant mutants of Saccharomyces cerevisiae by in vivo evolutionary engineering approach. Journal of Biotechnology, 2009, 143(2): 130-138
CrossRef Google scholar
[12]
Gilbert A, Sangurdekar D P, Srienc F. Rapid strain improvement through optimized evolution in the cytostat. Biotechnology and Bioengineering, 2009, 103(3): 500-512
CrossRef Google scholar
[13]
Wisselink H W, Toirkens M J, Wu Q, Pronk J T, Maris A JA. Novel evolutionary engineering approach for accelerated utilization of glucose, xylose, and arabinose mixtures by engineered Saccharomyces cerevisiae strains. Applied and Environmental Microbiology, 2009, 75(4): 907-914
CrossRef Google scholar

Acknowledgments

This work was supported by the National Basic Research Program of China (973) (Grant No. 2007CB707802), and the National Natural Science Foundation of China (Grant Nos. 20806055, 20875068).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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