Frontiers of Chemical Science and Engineering >
The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition
Received date: 16 Jun 2011
Accepted date: 26 Feb 2012
Published date: 05 Jun 2012
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The role of Escherichiacoli rpoS on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the rpoS-deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of gltA and one-tenth downregulation of poxB, respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing rpoS gene into the mutant. These results demonstrated rpoS changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.
Prayoga SURYADARMA , Yoshihiro OJIMA , Yuto FUKUDA , Naohiro AKAMATSU , Masahito TAYA . The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition[J]. Frontiers of Chemical Science and Engineering, 2012 , 6(2) : 152 -157 . DOI: 10.1007/s11705-012-1287-0
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