The
Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA
The
The role of Escherichiacoli rpoS on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the rpoS-deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of gltA and one-tenth downregulation of poxB, respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing rpoS gene into the mutant. These results demonstrated rpoS changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.
Escherichia coli / rpoS / aerobic and glucose-enriched culture / overflow metabolism
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