The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

PDF(175 KB)
PDF(175 KB)
Front. Chem. Sci. Eng. ›› 2012, Vol. 6 ›› Issue (2) : 152-157. DOI: 10.1007/s11705-012-1287-0
RESEARCH ARTICLE
RESEARCH ARTICLE

The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition

Author information +
History +

Abstract

The role of Escherichiacoli rpoS on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the rpoS-deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of gltA and one-tenth downregulation of poxB, respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing rpoS gene into the mutant. These results demonstrated rpoS changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.

Keywords

Escherichia coli / rpoS / aerobic and glucose-enriched culture / overflow metabolism

Cite this article

Download citation ▾
Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA. The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition. Front Chem Sci Eng, 2012, 6(2): 152‒157 https://doi.org/10.1007/s11705-012-1287-0

References

[1]
Hengge-Aronis R. Signal transduction and regulatory mechanisms involved in control of the σS (rpoS) subunit of RNA polymerase. Microbiology and Molecular Biology Reviews, 2002, 66(3): 373–395
CrossRef Pubmed Google scholar
[2]
Eisenstark A, Calcutt M J, Becker-Hapak M, Ivanova A. Role of Escherichia coli rpoS and associated genes in defense against oxidative damage. Free Radical Biology and Medicine, 1996, 21(7): 975–993
CrossRef Pubmed Google scholar
[3]
González-Flecha B, Demple B. Metabolic sources of hydrogen peroxide in aerobically growing Escherichia coli. Journal of Biological Chemistry, 1995, 270(23): 13681–13687
CrossRef Pubmed Google scholar
[4]
Imlay J A, Fridovich I. Assay of metabolic superoxide production in Escherichia coli. Journal of Biological Chemistry, 1991, 266(11): 6957–6965
Pubmed
[5]
Storz G, Imlay J A. Oxidative stress. Current Opinion in Microbiology, 1999, 2(2): 188–194
CrossRef Pubmed Google scholar
[6]
Chang Y Y, Wang A Y, Cronan J E Jr. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Molecular Microbiology, 1994, 11(6): 1019–1028
CrossRef Pubmed Google scholar
[7]
Patten C L, Kirchhof M G, Schertzberg M R, Morton R A, Schellhorn H E. Microarray analysis of rpoS-mediated gene expression in Escherichia coli K-12. Molecular Genetics and Genomics, 2004, 272(5): 580–591
CrossRef Pubmed Google scholar
[8]
Ueguchi C, Misonou N, Mizuno T. Negative control of rpoS expression by phosphoenolpyruvate: Carbohydrate phosphotransferase system in Escherichia coli. Journal of Bacteriology, 2001, 183(2): 520–527
CrossRef Pubmed Google scholar
[9]
Saka K, Tadenuma M, Nakade S, Tanaka N, Sugawara H, Nishikawa K, Ichiyoshi N, Kitagawa M, Mori H, Ogasawara N, Nishimura A. A complete set of Escherichia coli open reading frames in mobile plasmids facilitating genetic studies. DNA Research, 2005, 12(1): 63–68
CrossRef Pubmed Google scholar
[10]
Cooper C M, Fernstrom G A, Miller S A. Performance of agitated gas-liquid contactors. Industrial and Engineering Chemistry, 1944, 36(6): 504–509
CrossRef Google scholar
[11]
Ojima Y, Kawase D, Nishioka M, Taya M. Functionally undefined gene, yggE, alleviates oxidative stress generated by monoamine oxidase in recombinant Escherichia coli. Biotechnology Letters, 2009, 31(1): 139–145
CrossRef Pubmed Google scholar
[12]
Andersen K B, von Meyenburg K. Are growth rates of Escherichia coli in batch cultures limited by respiration? Journal of Bacteriology, 1980, 144(1): 114–123
Pubmed
[13]
Phue J N, Shiloach J. Impact of dissolved oxygen concentration on acetate accumulation and physiology of E. coli BL21, evaluating transcription levels of key genes at different dissolved oxygen conditions. Metabolic Engineering, 2005, 7(5–6): 353–363
CrossRef Pubmed Google scholar
[14]
Iuchi S, Lin E C. arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways. Proceedings of the National Academy of Sciences of the United States of America, 1988, 85(6): 1888–1892
CrossRef Pubmed Google scholar
[15]
Majewski R A, Domach M M. Simple constrained-optimization view of acetate overflow in Escherichia coli. Biotechnology and Bioengineering, 1990, 7(7): 732–738
CrossRef Google scholar
[16]
Britten R. Extracellular metabolic product of Escherichia coli during rapid growth. Science, 1954, 119: 578–578
[17]
Han K, Lim H C, Hong J. Acetic acid formation in Escherichia coli fermentation. Biotechnology and Bioengineering, 1992, 39(6): 663–671
CrossRef Pubmed Google scholar
[18]
Vemuri G N, Altman E, Sangurdekar D P, Khodursky A B, Eiteman M A. Overflow metabolism in Escherichia coli during steady-state growth: transcriptional regulation and effect of the redox ratio. Applied and Environmental Microbiology, 2006, 72(5): 3653–3661
CrossRef Pubmed Google scholar
[19]
Ingledew W J, Poole R K. The respiratory chains of Escherichia coli. Microbiological Reviews, 1984, 48(3): 222–271
Pubmed
[20]
Gray C T, Wimpenny J W, Mossman M R. Regulation of metabolism in facultative bacteria: II. Effects of aerobiosis, anaerbiosis and nutrition on the formation of Krebs cycle enzymes in Escherchia coli. Biochimica et Biophysica Acta. G, General Subjects, 1966, 1(1): 33–41
CrossRef Google scholar
[21]
Amarasingham C R, Davis B D. Regulation of α-ketoglutarate dehydrogenase formation in Escherichia coli. Journal of Biological Chemistry, 1965, 240(9): 3664–3668
Pubmed

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
AI Summary AI Mindmap
PDF(175 KB)

Accesses

Citations

Detail

Sections
Recommended

/