Biological pretreatment of corn stover by solid state fermentation of Phanerochaete chrysosporium

Abstract

Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the supermolecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes (LiP and MnP) were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are useful to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.

Key words： biological pretreatment; Phanerochaete chrysosporium; solid state fermentation; biorecalcitrance; hydrolysis yield

Acknowledgments

This research was supported by the State Key Development Drogram for Basic Research of China (Grant No. 2011CB707406), the National Natural Science Foundation of China (20976051), Ministry of Education of China (Grant No. 20090074110013), the Shanghai Leading Academic Discipline Project (B505), and the Fundamental Research Funds for the Central Universities of China (WF0913005).

References

1 Kirk T K, Farrell R L. Enzymatic “combustion”: the microbial degradation of lignin. Annual Review of Microbiology, 1987, 41(1): 465–501

2 Gold M H, Alic M. Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium. MicroBiologicalal Reviews, 1993, 57: 605–622

3 Martinez D, Larrondo L F, Putnam N, Gelpke M D S, Huang K, Chapman J, Helfenbein K G, Ramaiya P, Detter J C, Larimer F, Coutinho P M, Henrissat B, Berka R, Cullen D, Rokhsar D. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nature Biotechnology, 2004, 22(6): 695–700

4 Xu F, Chen H, Li Z. Solid-state production of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium using steam-exploded straw as substrate. Bioresource Technology, 2001, 80(2): 149–151

5 Kumar A G, Sekaran G, Krishnamoorthy S. Solid state fermentation of Achras zapota lignocellulose by Phanerochaete chrysosporium. Bioresource Technology, 2006, 97(13): 1521–1528

6 Kirk T K, Schultz E, Connors W J, Lorenz L F, Zeikus J G. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Archives of Microbiology, 1978, 117(3): 277–285

7 Arora D S, Chander M, Gill P K. Involvement of lignin peroxidase, manganese peroxidase and laccase in degradation and selective ligninolysis of wheat straw. International Biodeterioration & Biodegradation, 2002, 50(2): 115–120

8 Kapich A N, Prior B A, Botha A, Galkin S, Lundell T, Hatakka A. Effect of lignocellulose-containing substrates on production of ligninolytic peroxidases in submerged cultures of Phanerochaete chrysosporium ME-446. Enzyme and Microbial Technology, 2004, 34(2): 187–195

9 Wood W A, Kellogg S T. Methods in Enzymology-Biomass. Part B: lignin, pectin, and chitin. San Diego: Academic Press, 1988, 238–249

10 National Renewable Energy Laboratory (NREL). 1996. Measurement of cellulase activities (LAP-006). Golden, CO, USA

11 National Renewable Energy Laboratory (NREL). 2005. Determination of structural carbohydrates and lignin in biomass. Golden, CO, USA

12 National Renewable Energy Laboratory (NERL). 1996. Chemical analysis and testing task laboratory analytical procedure: enzymatic saccharification of lignocellulosic biomass. Golden, CO, USA

13 Jager A, Croan S, Kirk T K. Production of ligninase and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium. Applied and Environmental Microbiology, 1985, 50: 1274–1278

14 Shi J, Sharma-Shivappa R R, Chinn M, Howell N. Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass and Bioenergy, 2009, 33(1): 88–96

15 Wan C, Li Y. Effect of hot water extraction and liquid hot water pretreatment on the fungal degradation of biomass feedstocks. Bioresource Technology, 2011, 102(20): 9788–9793

16 Zhang J, Zhu Z N, Wang X F, Wang N, Wang W, Bao J. Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation. Biotechnology for Biofuels, 2010, 3(1): 26 doi:10.1186/1754-6834-3-26

17 Wan C X, Li Y B. Microbial pretreatment of corn stover with Ceriporiopsis subvermispora for enzymatic hydrolysis and ethanol production. Bioresource Technology, 2010, 101(16): 6398–6403

18 Reid I D. Solid-state fermentations for Biologicalal delignification. Enzyme and Microbial Technology, 1989, 11(12): 786–803

19 Shi J, Chinn M S, Sharma-Shivappa R R. Microbial pretreatment of cotton stalks by solid state cultivation of Phanerochaete chrysosporium. Bioresource Technology, 2008, 99(14): 6556–6564

20 Asgher M, Asad M J, Legge R L. Enhanced lignin peroxidase synthesis by Phanerochaete chrysosporium in solid state bioprocessing of a lignocellulosic substrate. World Journal of Microbiology & Biotechnology, 2006, 22(5): 449–453

21 Singhania R R, Patel A K, Soccol C R, Pandey A. Recent advances in solid state fermentation. Biochemical Engineering Journal, 2009, 44(1): 13–18

22 Zeng J J, Singh D, Chen S L. Biologicalal pretreatment of wheat straw by Phaerochaete chrysporium supplemented with inorganic salts. Bioresource Technology, 2011, 102(3): 3206–3214

23 Buswell J A, Odier E, Kirk T K. Lignin biodegradation. Critical Reviews in Biotechnology, 1987, 6(1): 1–60 doi:10.3109/07388558709086984

24 Stewart P, Cullen D. Organization and differential regulation of a cluster of lignin peroxidase genes of Phanerochaete chrysosporium. Journal of Bacteriology, 1999, 181: 3427–3432

25 Xu C Y, Ma F Y, Zhang X Y, Chen S L. Biologicalal pretreatment of corn stover by Irpex lacteus for enzymatic hydrolysis. Journal of Agricultural and Food Chemistry, 2010, 58(20): 10893–10898

26 Bak J S, Ko J K, Choi I G, Park Y C, Seo J H, Kim K H. Fungal pretreatment of lignocellulose by Phanerochaete charysosporium to produce ethanol from rice straw. Biotechnology and Bioengineering, 2009, 104(3): 471–482