Biological pretreatment of corn stover by solid state fermentation of Phanerochaete chrysosporium

Abstract

Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the supermolecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes (LiP and MnP) were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are useful to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.

Keywords

biological pretreatment / Phanerochaete chrysosporium / solid state fermentation / biorecalcitrance / hydrolysis yield

References

[1] Kirk T K, Farrell R L. Enzymatic “combustion”: the microbial degradation of lignin. Annual Review of Microbiology, 1987, 41(1): 465–501

[2] Gold M H, Alic M. Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium. MicroBiologicalal Reviews, 1993, 57: 605–622

[3] Martinez D, Larrondo L F, Putnam N, Gelpke M D S, Huang K, Chapman J, Helfenbein K G, Ramaiya P, Detter J C, Larimer F, Coutinho P M, Henrissat B, Berka R, Cullen D, Rokhsar D. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nature Biotechnology, 2004, 22(6): 695–700

[4] Xu F, Chen H, Li Z. Solid-state production of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium using steam-exploded straw as substrate. Bioresource Technology, 2001, 80(2): 149–151

[5] Kumar A G, Sekaran G, Krishnamoorthy S. Solid state fermentation of Achras zapota lignocellulose by Phanerochaete chrysosporium. Bioresource Technology, 2006, 97(13): 1521–1528

[6] Kirk T K, Schultz E, Connors W J, Lorenz L F, Zeikus J G. Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Archives of Microbiology, 1978, 117(3): 277–285

[7] Arora D S, Chander M, Gill P K. Involvement of lignin peroxidase, manganese peroxidase and laccase in degradation and selective ligninolysis of wheat straw. International Biodeterioration & Biodegradation, 2002, 50(2): 115–120

[8] Kapich A N, Prior B A, Botha A, Galkin S, Lundell T, Hatakka A. Effect of lignocellulose-containing substrates on production of ligninolytic peroxidases in submerged cultures of Phanerochaete chrysosporium ME-446. Enzyme and Microbial Technology, 2004, 34(2): 187–195

[9] Wood W A, Kellogg S T. Methods in Enzymology-Biomass. Part B: lignin, pectin, and chitin. San Diego: Academic Press, 1988, 238–249

[10] National Renewable Energy Laboratory (NREL). 1996. Measurement of cellulase activities (LAP-006). Golden, CO, USA

[11] National Renewable Energy Laboratory (NREL). 2005. Determination of structural carbohydrates and lignin in biomass. Golden, CO, USA

[12] National Renewable Energy Laboratory (NERL). 1996. Chemical analysis and testing task laboratory analytical procedure: enzymatic saccharification of lignocellulosic biomass. Golden, CO, USA

[13] Jager A, Croan S, Kirk T K. Production of ligninase and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium. Applied and Environmental Microbiology, 1985, 50: 1274–1278

[14] Shi J, Sharma-Shivappa R R, Chinn M, Howell N. Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass and Bioenergy, 2009, 33(1): 88–96

[15] Wan C, Li Y. Effect of hot water extraction and liquid hot water pretreatment on the fungal degradation of biomass feedstocks. Bioresource Technology, 2011, 102(20): 9788–9793

[16] Zhang J, Zhu Z N, Wang X F, Wang N, Wang W, Bao J. Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation. Biotechnology for Biofuels, 2010, 3(1): 26 doi:10.1186/1754-6834-3-26

[17] Wan C X, Li Y B. Microbial pretreatment of corn stover with Ceriporiopsis subvermispora for enzymatic hydrolysis and ethanol production. Bioresource Technology, 2010, 101(16): 6398–6403

[18] Reid I D. Solid-state fermentations for Biologicalal delignification. Enzyme and Microbial Technology, 1989, 11(12): 786–803

[19] Shi J, Chinn M S, Sharma-Shivappa R R. Microbial pretreatment of cotton stalks by solid state cultivation of Phanerochaete chrysosporium. Bioresource Technology, 2008, 99(14): 6556–6564

[20] Asgher M, Asad M J, Legge R L. Enhanced lignin peroxidase synthesis by Phanerochaete chrysosporium in solid state bioprocessing of a lignocellulosic substrate. World Journal of Microbiology & Biotechnology, 2006, 22(5): 449–453

[21] Singhania R R, Patel A K, Soccol C R, Pandey A. Recent advances in solid state fermentation. Biochemical Engineering Journal, 2009, 44(1): 13–18

[22] Zeng J J, Singh D, Chen S L. Biologicalal pretreatment of wheat straw by Phaerochaete chrysporium supplemented with inorganic salts. Bioresource Technology, 2011, 102(3): 3206–3214

[23] Buswell J A, Odier E, Kirk T K. Lignin biodegradation. Critical Reviews in Biotechnology, 1987, 6(1): 1–60 doi:10.3109/07388558709086984

[24] Stewart P, Cullen D. Organization and differential regulation of a cluster of lignin peroxidase genes of Phanerochaete chrysosporium. Journal of Bacteriology, 1999, 181: 3427–3432

[25] Xu C Y, Ma F Y, Zhang X Y, Chen S L. Biologicalal pretreatment of corn stover by Irpex lacteus for enzymatic hydrolysis. Journal of Agricultural and Food Chemistry, 2010, 58(20): 10893–10898

[26] Bak J S, Ko J K, Choi I G, Park Y C, Seo J H, Kim K H. Fungal pretreatment of lignocellulose by Phanerochaete charysosporium to produce ethanol from rice straw. Biotechnology and Bioengineering, 2009, 104(3): 471–482

Acknowledgments

This research was supported by the State Key Development Drogram for Basic Research of China (Grant No. 2011CB707406), the National Natural Science Foundation of China (20976051), Ministry of Education of China (Grant No. 20090074110013), the Shanghai Leading Academic Discipline Project (B505), and the Fundamental Research Funds for the Central Universities of China (WF0913005).

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