Application of statistical design for the production of inulinase by streptomyces sp. using pressmud

Abstract

A Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using streptomyces sp. and pressmud as the substrate via solid-state fermentation (SSF). From the experiments, three nutrients viz. yeast extract, FeSO₄·7H₂O, and NH₄NO₃ were found to be the most significant components. Hence these three components were selected and optimized using Response Surface Methodology (RSM). The optimum conditions are: yeast extract 0.00274 g/gds, FeSO₄·7H₂O 0.00011 g/gds and NH₄NO₃ 0.00772 g/gds. The effect of the substrate concentration and initial moisture content were also studied. A substrate concentration of 12 g and an initial moisture content of 65% are optimum for the maximum production of inulinase (89 U/gds).

Key words： inulinase; pressmud; Response Surface Methodology (RSM); streptomyces sp

Acknowledgments

The authors acknowledge the research grant provided by the All India Council for Technical Education, through Research Promotion Scheme (RPS). The authors wish to express their gratitude for the support extended by the authorities of Annamalai University, Annamalainagar, India in carrying out the research work in the Environmental Engineering laboratory, Department of Chemical Engineering.

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