Application of statistical design for the production of inulinase by streptomyces sp. using pressmud

Abstract

A Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using streptomyces sp. and pressmud as the substrate via solid-state fermentation (SSF). From the experiments, three nutrients viz. yeast extract, FeSO₄·7H₂O, and NH₄NO₃ were found to be the most significant components. Hence these three components were selected and optimized using Response Surface Methodology (RSM). The optimum conditions are: yeast extract 0.00274 g/gds, FeSO₄·7H₂O 0.00011 g/gds and NH₄NO₃ 0.00772 g/gds. The effect of the substrate concentration and initial moisture content were also studied. A substrate concentration of 12 g and an initial moisture content of 65% are optimum for the maximum production of inulinase (89 U/gds).

Keywords

inulinase / pressmud / Response Surface Methodology (RSM) / streptomyces sp

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