Frontiers of Chemical Science and Engineering >
Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae
Received date: 25 Jun 2016
Accepted date: 10 Aug 2016
Published date: 17 Mar 2017
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Metabolic engineering of heterologous resveratrol production in Saccharomyces cerevisiae faces challenges as the precursor L-tyrosine is stringently regulated by a complex biosynthetic system. We overexpressed the main gene targets in the upstream pathways to investigate their influences on the downstream resveratrol production. Single-gene overexpression and DNA assembly-directed multigene overexpression affect the production of resveratrol as well as its precursor p-coumaric acid. Finally, the collaboration of selected gene targets leads to an optimal resveratrol production of 66.14±3.74 mg·L–1, 2.27 times higher than the initial production in YPD medium (4% glucose). The newly discovered gene targets TRP1 expressing phosphoribosylanthranilate isomerase, ARO3 expressing 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, and 4CL expressing 4-coumaryl-CoA ligase show notable positive impacts on resveratrol production in S. cerevisiae.
Duo Liu , Bingzhi Li , Hong Liu , Xuejiao Guo , Yingjin Yuan . Profiling influences of gene overexpression on heterologous resveratrol production in Saccharomyces cerevisiae[J]. Frontiers of Chemical Science and Engineering, 2017 , 11(1) : 117 -125 . DOI: 10.1007/s11705-016-1601-3
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