Isolation, identification and primary application of bacteria from putrid alkaline silica sol

Abstract

The putrefaction of alkaline silica sol was investigated in this paper. The total colony numbers in three alkaline silica sol samples were 1.47×10⁵, 1.25×10⁴, and 9.45×10⁴ cfu·mL^–1, respectively. The salt- and alkali-tolerant strains were isolated and selected using nutrient agar medium at 2.5% salinity and pH 9.5. Basic morphological, physiological and biochemical tests were conducted to confirm the preliminary characterizations of the strains. Based on API 50 CH test and 16S rDNA gene sequence analysis, the isolated strains were finally identified as Exiguobacterium aurantiacum, Cyclobacteriaceae bacterium, Microbacterium sp., Acinetobacter sp., Stenotrophomonas maltophilia and Bacillus thuringiensis. The survivability of the strains under different conditions such as salinities, acidities and temperatures was also studied. Some suitable methods for degerming, such as product pipe steam sterilization and regular canister cleaning, were proposed. To explore the possibility of isolates in industrial application, their alkaline protease and amylase production abilities were preliminarily studied. Five strains produced alkaline protease, whereas two strains produced alkaline amylase. Thus, understanding of the putrefaction on alkaline silica sol would be beneficial for improving industrial production.

Key words： putrid alkaline silica sol; alkaliphile; isolation and identification; sterilization; alkaline protease and alkaline amylase

Acknowledgments

The authors would like to thank Department of Food Science, School of Chemical Engineering and Technology, Tianjin University for providing researching grants, whose invaluable supports made this study possible.

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