EXTRACTION AND EVALUATION OF EDIBLE OIL FROM SCHIZOCHYTRIUM SP. USING AN AQUEOUS ENZYMATIC METHOD

Highlights

• Aqueous enzymatic extraction (AEE) is performed for oil extraction from Schizochytrium sp.

• AEE process is optimized by response surface methodology.

• Microalgal oil extracted by AEE has high contents of PUFA, tocopherols and phenolics.

• AEEO exhibits considerable antioxidant activity as compared with SEO.

Abstract

Schizochytrium sp., a marine microalga, is a potential source of edible oil due to its short growth cycle and rapid lipid accumulation, especially of docosahexaenoic acid. An approach to isolate edible microalgal oil from Schizochytrium sp. using aqueous enzymatic extraction (AEE) was developed. Parameters were optimized by single-factor experiments followed by Box-Behnken design. Proteases were effective in extracting oil. The maximum free oil recovery (49.7%±0.58%) and total oil recovery (68.1%±0.94%) were obtained under optimum conditions of liquid-to-solid ratio of 4.8:1, a 2.5% enzyme concentration of papain and an extraction time of 2.2 h. There was a significant difference (P<0.05) in polyunsaturated fatty acid composition between microalgal oil obtained by AEE and by Soxhlet extraction, with the former having superior physiochemical properties and higher concentrations of bioactive components including total phenolic compounds and total tocopherols. These findings indicate a potential application of AEE for extraction of oil from Schizochytrium sp.

Graphical abstract

Keywords

antioxidant activity / aqueous enzymatic extraction / edible microalgal oil / fatty acid composition / physicochemical properties

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Supplementary materials

The online version of this article at https://doi.org/10.15302/J-FASE-2021400 contains supplementary materials (Tables S1–S3).

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (31571825, 31271979), the Natural Science Foundation of Tianjin (15JCYBJC30100), the Innovation Foundation of Tianjin University (2017XZC-0017), and the Dean Foundation of Tianjin Academy of Agricultural Sciences (14006).

Compliance with ethics guidelines

Zhaohui Xue, Fang Wan, Xin Gao, Wancong Yu, Zhijun Zhang, Jing Liu, and Xiaohong Kou declare that they have no conflicts of interest or financial conflicts to disclose. This article does not contain any studies with human or animal subjects performed by any of the authors.

RIGHTS & PERMISSIONS

The Author(s) 2021. Published by Higher Education Press. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0)