Porcine pluripotent stem cells: progress, challenges and prospects

Abstract

Pluripotent stem cells (PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scientific and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding.

Keywords

embryonic germ cells / embryonic stem cells / induced pluripotent stem cells / pigs / pluripotent stem cells

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Acknowledgements

This work was funded by the National Key Research and Development Program of China-Stem Cell and Translational Research (2016YFA0100200).

Compliance with ethics guidelines

Jianyong Han, Yi-Liang Miao, Jinlian Hua, Yan Li, Xue Zhang, Jilong Zhou, Na Li, Ying Zhang, Jinying Zhang, and Zhonghua Liu declare that they have no conflicts of interest or financial conflicts to disclose.

This article is a review and does not contain any studies with human or animal subjects performed by any of the authors.

RIGHTS & PERMISSIONS

The Author(s) 2018. Published by Higher Education Press. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0)