Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Abstract

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenic animals. A key component in the technology is the construction of an efficient milk expression vector. Here, we established a simple method to construct a milk expression vector, by a combination of homologous recombination and digestion-ligation. Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome (BAC) vectors. The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombination to produce a universal expression vector, and the human lysozyme gene (hLZ) was then inserted into the vector by a digestion-ligation method. The final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0 kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk. The highest expression level of rhLZ was 0.45 g·L⁻¹, and rhLZ exhibited the same antibacterial activity as native hLZ. Our results have provided a simple approach to construct a universal milk expression vector, and demonstrated that the resulting vector regulates the expression of hLZ in milk.

Keywords

BAC recombinant methods / gene expression / human lysozyme / transgenic mice / milk expression vector

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Acknowledgements

We would like to thank Dr. Hongxing Chen for technical advice and his contribution of mWAP clone. This work was supported by High-Level Talent Project of Foshan University (GG040965), Science and Technology Project of Foshan City (GS54207) and Science Foundation of Jiangsu Province (BK20160206).

Compliance with ethics guidelines

ƒShen Liu, Shengzhe Shang, Xuezhen Yang, Huihua Zhang, Dan Lu, and Ning Li declare that they have no conflicts of interest or financial conflicts to disclose.ƒAll applicable institutional and national guidelines for the care and use of animals were followed.

RIGHTS & PERMISSIONS

The Author(s) 2018. Published by Higher Education Press. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0)