Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum

Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU

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Front. Agr. Sci. Eng. ›› 2018, Vol. 5 ›› Issue (3) : 362-372. DOI: 10.15302/J-FASE-2018206
RESEARCH ARTICLE
RESEARCH ARTICLE

Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum

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Abstract

This study compared proteome profiles and morphological changes of rat jejunum in response to different dietary proteins. Fifty male Sprague-Dawley rats were fed with casein (control), and isolated beef, pork, fish and chicken proteins for 14 days. Proteome analysis, histological observation and PEPT1 quantification of the jejunum were performed. The results indicated that rats fed with chicken proteins had higher PEPT1 mRNA and protein levels (P<0.05) but lower villus height and ratio of villus height to crypt depth (V/C ratio, P<0.05) than those fed with casein and pork protein. Label-free LC-MS/MS indicated that, as compared to casein, intake of chicken protein can regulate oligopeptide transport mainly by upregulating PEPT1 protein expression and reducing dipeptidyl-peptidase activity related to biological oxidation, and can reduce oligopeptide absorption capacity by regulating Hippo signaling pathway. Although intake of beef and fish proteins had no significant effect on PEPT1 expression, they altered several signaling pathways.

Keywords

Hippo signaling pathway / meat protein / PEPT1 / proteome analysis / rat jejunum

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Mengjie LI, Chunbao LI, Shangxin SONG, Xinglian XU, Guanghong ZHOU. Proteome comparisons reveal influence of different dietary proteins on the development of rat jejunum. Front. Agr. Sci. Eng., 2018, 5(3): 362‒372 https://doi.org/10.15302/J-FASE-2018206

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Supplementary materials

The online version of this article at https://doi.org/10.15302/J-FASE-2018206 contains supplementary materials (Tables S1–S4).

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31471600, 31530054).

Compliance with ethics guidelines

Mengjie Li, Chunbao Li, Shangxin Song, Xinglian Xu, and Guanghong Zhou declare that they have no conflicts of interest or financial conflicts to disclose.
All applicable institutional and national guidelines for the care and use of animals were followed.

RIGHTS & PERMISSIONS

The Author(s) 2018. Published by Higher Education Press. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0)
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