Efficient regeneration system applicable to five Musa cultivars

Abstract

Banana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Hybridization is seriously hampered by the long generation time, polyploidy, and sterility of most cultivars. Establishment of an efficient regeneration and transformation system for banana is critical for their genetic improvement. An efficient and reproducible transformation system for banana using direct organogenesis was developed. Media containing benzylaminopurine (BA) combined with one of four other growth regulators was evaluated for the regeneration efficiency of five Musa cultivars and the ability to induce/support development of new banana shoots. The result indicated that the greatest number of shoots per explant for all five Musa cultivars was obtained using MS medium supplemented with 8.9 mmol·L⁻¹ BA and 9.1 mmol·L⁻¹ thidiazuron (TDZ). In 240–270 d, one immature male flower could regenerate between 380 and 456, 310–372, 200–240, 130–156, and 100–130 well-developed shoots for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Such a system will facilitate molecular breeding and functional genomics of banana.

Keywords

banana (Musa spp) / system / regeneration

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Acknowledgements

This work was supported by the earmarked fund for Modern Agro-Industry Technology Research System (CARS-32) and the National Nonprofit Institute Research Grant of the Institute of Tropical Bioscience and Biotechnology CATAS-ITBB (ITBB2015ZY06).

Compliance with ethics guidelines

Juhua Liu, Peiguang Sun, Jing Zhang, Jiashui Wang, Jianbin Zhang, Jingyi Wang, Caihong Jia, Pengzhao Gao, Biyu Xu, and Zhiqiang Jin declare they have no conflicts of interest or financial conflicts to disclose.

This article does not contain any studies with human or animal subjects performed by any of the authors.

RIGHTS & PERMISSIONS

The Author(s) 2016. Published by Higher Education Press. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0)