Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine

Jianxiang XU, Dengke PAN, Jie ZHAO, Jianwu WANG, Xiaohong HE, Yuehui MA, Ning LI

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Front. Agr. Sci. Eng. ›› 2015, Vol. 2 ›› Issue (1) : 90-99. DOI: 10.15302/J-FASE-2015045
RESEARCH ARTICLE
RESEARCH ARTICLE

Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine

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Abstract

Myostatin is a transforming growth factor-β family member that normally acts to limit skeletal muscle growth. Myostatin gene (MSTN) knockout (KO) mice show possible effects for the prevention or treatment of metabolic disorders such as obesity and type 2 diabetes. We applied chromatography and mass spectrometry based metabonomics to assess system-wide metabolic response of heterozygous MSTN KO (MSTN+/-) swine. Most of the metabolic data for MSTN+/- swine were similar to the data for wild type (WT) control swine. There were, however, metabolic changes related to fatty acid metabolism, glucose utilization, lipid metabolism, as well as BCAA catabolism caused by monoallelic MSTN depletion.The statistical analyses suggested that: (1) most metabolic changes were not significant in MSTN+/- swine compared to WT swine; (2) only a few metabolic properties were significantly different between KO and WT swine, especially for lipid metabolism. Significantly, these minor changes were most evident in female KO swine and suggested differences in gender sensitivity to myostatin.

Keywords

myostatin / transforming growth factor-β family / skeletal muscle / metabolic disorders / chromatography / mass spectrometry / metabonomics

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Jianxiang XU, Dengke PAN, Jie ZHAO, Jianwu WANG, Xiaohong HE, Yuehui MA, Ning LI. Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine. Front. Agr. Sci. Eng., 2015, 2(1): 90‒99 https://doi.org/10.15302/J-FASE-2015045

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Acknowledgments

This work was funded by the key special projects of breeding new varieties of genetically modified organisms in China (2014ZX08012-002). We thank Metabolon, a diagnostic products and services company, for assistance with the metabolic profile and statistical analysis.
Supplementary materialsƒThe online version of this article at http://dx.doi.org/10.15302/J-FASE-2015045 contains supplementary material (Appendix A).
Compliance with ethics guidelinesƒJianxiang Xu, Dengke Pan, Jie Zhao, Jianwu Wang, Xiaohong He, Yuehui Ma and Ning Li declare that they have no conflict of interest or financial conflicts to disclose.
ƒAll applicable institutional and national guidelines for the care and use of animals were followed.

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