Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division cycle

Abstract

Foot-and-mouth-disease virus (FMDV) replicates in epithelial cells. The restriction of FMDV RNA to the basal cell layer of epithelia suggests a possible link between FMDV replication in vivo and the cell status. This paper describes in vitro studies in which FMDV infection was investigated in cells that were held at various cell division phases using cell cycle inhibitors. The results suggest that when cells were arrested at the G₁ or G₁/S phase, high levels of viral RNA were detected by quantitative real-time reverse transcription PCR and viral protein synthesis was observed by specific labeling techniques. In contrast, when cells were arrested at the G₂/M phase, reduced or no viral RNA synthesis was detected.

Keywords

foot-and-mouth disease virus / cell cycle / replication

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Acknowledgements

This work was mainly supported by the Biotechnology and Biological Sciences Research Council (China partnership award and project BB/E003435/1), UK.

ƒClaudia Doel, Zhidong Zhang, Lise Mazelet, Ryan Waters and John Bashiruddin declare that they have no conflict of interest or financial conflicts to disclose.ƒAll applicable institutional and national guidelines for the care and use of animals were followed.

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Higher Education Press and Springer-Verlag Berlin Heidelberg