Progress, problems and prospects of porcine pluripotent stem cells

Abstract

Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), can differentiate into cells of the three germ layers, suggesting that PSCs have great potential for basic developmental biology research and wide applications for clinical medicine. Genuine ESCs and iPSCs have been derived from mice and rats, but not from livestock such as the pig—an ideal animal model for studying human disease and regenerative medicine due to similarities with human physiologic processes. Efforts to derive porcine ESCs and iPSCs have not yielded high-quality PSCs that can produce chimeras with germline transmission. Thus, exploration of the unique porcine gene regulation network of preimplantation embryonic development may permit optimization of in vitro culture systems for raising porcine PSCs. Here we summarize the recent progress in porcine PSC generation as well as the problems encountered during this progress and we depict prospects for generating porcine naive PSCs.

Keywords

induced pluripotent stem cells / embryonic stem cells / pig / reprogramming

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Acknowledgments

This work was funded by the National Key Basic Research and Development Program of China (2010CB945404, 2011CBA01001 and 2009CB941003), the Chinese Universities Scientific Fund (2012RC014), and the National Thousand Talents Program of China. There are no conflicts of interest or financial conflicts to disclose.

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