Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost

Abstract

Spring radiation frost (SRF) is a severe environmental stress which impairs wheat yield and productivity worldwide. To better understand the mechanism of wheat (Triticum aestivum) responding to SRF, a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively. A total of 43 differentially expressed proteins (DEPs) which mainly involved in carbohydrate metabolism, amino acid metabolism, resistance proteins and antioxidant enzymes, photosynthesis and cellular respiration proteins, cell-wall related proteins, protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF MS. The results revealed that of the 21 DEPs in Jimai22 responding to the SRF, 13 DEPs were upregulated and 8 DEPs were downregulated, and that of the 22 DEPs in Luyuan301, 9 DEPs were upregulated and 13 DEPs were downregulated. These DEPs might be responsible for the stronger cold resistance of Jimai22 compared to Luyuan301. The expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat.

Keywords

common wheat / spring radiation frost / proteomic analysis / 2-DE / MALDI-TOF-TOF MS

References

[1] James PS, Morrel EM, Gary MP. Spring Freeze Injury to Kansas Wheat. Manhattan: Kansas state University, 1995.

[2] Slafer G A, Rawson H M. Base and optimum temperatures vary with genotype and stage of development in wheat. Plant, Cell & Environment, 1995, 18(6): 671-679

[3] Mahfoozi S, Limin A E, Fowler D B. Influence of vernalization and photoperiod responses on cold hardiness in winter cereals. Crop Science, 2001, 41(4): 1006-1011

[4] Fowler D B, Limin A E. Interactions among factors regulating phonological development and acclimation rate determine low-temperature tolerance in wheat. Annals of Botany, 2004, 94(5): 717-724

[5] Single W V. Variation in resistance to spring radiation frost in Triticum aestivum L. Australian Academy of Science, 1974, 282-287.

[6] Gusta L V, Chen T H H. The physiology of water and temperature stress. In wheat and wheat improvement E.G. Heyne (ed.). 2nd edition, ASA, CSSA, SSSA, Madison, WI, USA. 1987: 15-150.

[7] Single W V, Marcellos H. Studies on frost injury to wheat. Freezing of ears after emergence from the leaf sheath. Australian Journal of Agricultural Research, 1974, 25(5): 679

[8] Galiba G, Quarrie S A, Sutka J, Morgounov A, Snape J W. RFLP mapping of the vernalization (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat. Theoretical and Applied Genetics, 1995, 90(7-8): 1174-1179

[9] Galiba G, Vágújfalvi A, Li C, Soltész A, Dubcovsky J. Regulatory genes involved in the determination of frost tolerance in temperate cereals. Plant Science, 2009, 176(1): 12-19

[10] Sutka J, Galiba G, Vagujfalvi A, Gill B S, Snape J W. Physical mapping of the Vrn-A1 and Fr1 genes on chromosome 5A of wheat using deletion lines. Theoretical and Applied Genetics, 1999, 99(1-2): 199-202

[11] Tóth B, Galiba G, Fehér E. sutka J, snape JW. Mapping genes affecting flowering time and frost resistance on chromosome 5B of wheat. Theoretical and Applied Genetics, 2003, 107: 509-514

[12] Stockinger E J, Skinner J S, Gardner K G, Francia E, Pecchioni N. Expression levels of barley CBF genes at the frost resistance-H2 locus are dependent upon alleles at Fr-H1 and Fr-H2. Plant Journal, 2007, 51(2): 308-321

[13] Steponkus P L. Role of plasma membrane in cold acclimation and freezing injury in plants. Annual Review of Plant Physiology, 1984, 35: 543-584

[14] Campbell S A, Close T J. Dehydrins: genes, proteins, and association with phenotypic traits. New Phytologist, 1997, 137(1): 61-74

[15] Sãulescu N N, Braun H J. Breeding wheat for cold tolerance. In: Plant breeding for water-limited environments. Springer Publishers, Germany. 2001, 111-123.

[16] Gao L Y, Wang A L, Li X H, Dong K, Wang K, Appels R, Ma W J, Yan Y M. Wheat quality related differential expressions of albumins and globulins revealed by two-dimensional difference gel electrophoresis (2-D DIGE). Proteomics, 2009, 73(2): 279-296

[17] Nadaud I, Girousse C, Debiton C, Chambon C, Bouzidi M F, Martre P, Branlard G. Proteomic and morphological analysis of early stages of wheat grain development. Proteomics, 2010, 10(16): 2901-2910

[18] Tasleem-Tahir A, Nadaud I, Girousse C, Martre P, Marion D, Branlard G. Proteomic analysis of peripheral layers during wheat (Triticum aestivum L.) grain development. Proteomics, 2011, 11(3): 371-379

[19] Laino P, Shelton D, Finnie C, De Leonardis A M, Mastrangelo A M, Svensson B, Lafiandra D, Masci S. Comparative proteome analysis of metabolic proteins from seeds of durum wheat (cv. Svevo) subjected to heat stress. Proteomics, 2010, 10(12): 2359-2368

[20] Wang Y, Qian Y, Hu H, Xu Y, Zhang H. Comparative proteomic analysis of Cd-responsive proteins in wheat roots. Acta Physiologiae Plantarum, 2011, 33(2): 349-357

[21] Peng Z Y, Wang M C, Li F, Lv H J, Li C L, Xia G M. A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat. Molecular & Cellular Proteomics, 2009, 8(12): 2676-2686

[22] Demirevska K, Zasheva D, Dimitrov R, Simova-Stoilova L, Stamenova M, Feller U. Drought stress effects on Rubisco in wheat: changes in the Rubisco large subunit. Acta Physiologiae Plantarum, 2009, 31(6): 1129-1138

[23] Irar S, Brini F, Goday A, Masmoudi K, Pagès M. Proteomic analysis of wheat embryos with 2-DE and liquid-phase chromatography (ProteomeLab PF-2D)-A wider perspective of the proteome. Proteomics, 2010, 73(9): 1707-1721

[24] Danyluk J, Rassart E, Sarhan F. Gene expression during cold and heat shock in wheat. Biochemistry and Cell Biology, 1991, 69(5-6): 383-391

[25] Rinalducci S, Egidi M G, Karimzadeh G, Jazii F R, Zolla L. Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress. Electrophoresis, 2011, 32(14): 1807-1818

[26] Schonfeld M A, Johnson R C, Carver B F, Mornhinweg D W. Water relations in winter wheat as drought resistance indicator. Crop Science, 1988, 28(3): 526-531

[27] Yemm E W, Willis A J. The estimation of carbohydrates in plant extracts by the anthrone. Biochemistry, 1954, 57: 508-514

[28] Fan W, Zhang Z L, Zhang Y L. Cloning and molecular characterization of fructose-1.6-bisphosphate aldolase gene regulated by high-salinity and drought in Sesuvium portulacastrum. Plant Cell Reports, 2009, 28 (6): 975-984

[29] Liang L Q, Chang Y M, Zou Q W, Lei Q Q. Cloning and correlation analysis of cold adaptation of NADH-Quinone Oxidoreductase 3 Subunit Gene from common Carp (Cyprinus Carpio). Journal of Jishou University (Natural Sciences Edition), 2009, 30(5): 77-81

[30] Geisler D A, Papke C, Obata T, Nunes-Nesi A, Matthes A, Schneitz K, Maximova E, Araujo W L, Fernie A R, Persson S. Down regulation of the delta-subunit reduces mitochondrial ATP synthase levels, alters respiration, and restricts growth and gametophyte development in Arabidopsis. Plant Cell, 2012, 24(7): 2792-2811

[31] Vallelian-Bindschedler L, Mösiger E, Métraux J P, Schweizer P. Structure expression and localization of a germin-like protein in barley (Hordeum vulgare L.) that is insolubilized in stressed leaves. Plant Molecular Biology, 1998, 37(2): 297-308

[32] Mousavi A, Hotta Y. Glycine-rich proteins: a class of novel proteins. Applied Biochemistry and Biotechnology,2005, 120(3): 169-174

[33] Gallie D R. Post-transcriptional regulation of gene expression in plants.Annual Review of Plant Physiology and Plant Molecular Biology, 1993, 44(1): 77-105

[34] Yang Z.Small GTPases: versatile signaling switches in plants. The plant cell (Suppl), 2002, 14, S375–S388

Acknowledgments

This research was supported by Science & Technology Development Plan of Shandong Province (2013GNC11025), Shandong Agriculture and Seed Industry (2012), Funding for the Post-doctoral Innovative Projects of Shandong Province (201203024), the National Transgenic Major Project (2013ZX08002-004), China Agriculture Research System (CARS-03-1-08), Shandong Agriculture Research System, the national key technology R & D program of China (2011BAD35B03).

Supplementary material The online version of this article at http://dx.doi.org/(doi:) contains supplementary material, which is available to authorized users.

RIGHTS & PERMISSIONS

Higher Education Press and Springer-Verlag Berlin Heidelberg