At present, p53 gene and its product p53 protein are the hot spot in the field of cancer research. 50% human malignant tumors are associated with p53 alteration. p53 gene mutation is the most common mutation, which can lead to regulation disturbance of cell proliferation. Gastric cancer is the most common disease in China. Its incidence is the second among various malignant tumors. There were reports about p53 protein expression and gastric cancer progression but few report on p53 protein expression in different stages of precancerous lesions was available. In this study, we analyzed p53 protein expression in gastric cancer and precancerous lesions by flow cytometry to evaluate the role of p53 protein in the pathogenesis of gastric cancer.

An anti-metastatic polypeptide, bifunctional-domain (Cell I -Hep I) recombinant polypeptide of human fibronectin, was expressedin E. coli and purified. The expression level was found to be about 20%– 30 % of the total cell proteins. In BL21 (DE3)/T7, anE. coli expressing system, lactose can be used as an inducer to substitute IPTG thereby reducing the cost by several hundredfold and it is suitable for the large-scale preparation of recombinant FN polypeptide. Cell I -Hep 1 fragment is an alkaline polypeptide. In BL21(DE3)/T7 expressing system, better isolation was achieved if DEAE-52, instead of CM-52, was used for ion-exchange chromatography. The purified product was obtained after heparin-agarose affinity chromatography following ion-exchange chromatography.

The adenosine deaminase (ADA) activities in blood lymphocytes of 41 normal children and 17 with recurrent respiratory tract infections were examined, and the T-lymphocytes of two children whose ADA activities were obviously lower than those of others were culturedin vitro. Then the exogenous human ADA gene was transfected into these cells by means of lipofectin mediated gene transfer. The results showed that the ADA activities in cultured T-lymphocytes were raised and the immunological were also improved.

In order to investigate the role of nitric oxide (NO) in pathogenesis of hypoxic pulmonary hypertension (HPH), the mean pulmonary arterial pressure (mPAP), mRNA expression of NO synthase (NOS) in lung tissues, cGMP levels, and their relationships were studied in rats exposed to hypoxia from 8 h to 28 days. The results showed that mPAP began to increase in animals exposed to 10 % O₂ for 8 h. Moreover, the longer the exposure, the higher the mPAP. Northern blot analysis and dot blot hybridization indicated that mRNA expression of NO in lung tissues of hypoxic rats tended to decrease with exposure days, but that of β-actin which acted as a control did not alter. The cGMP levels of plasma and lung tissues in hypoxic rats also inclined to be lower with exposure days. A marked negative correlations between the changes of cGMP levels and those of mPAP were found. It was suggested that mRNA expression of NOS gene was attenuated in hypoxic lung tissues, which may be one of important pathogenetic mechanisms of HPH.

Presented in this paper is a biosensor constructed for the determination of neurotransmitters. It is an eggplant-banana-tissue-based carbon paste microelectrode. As oxidative enzymes in banana tissue can bio-catalytically oxidize dopamine (DA), norepinephrine (NE), L-dopa, etc., the biosensor’s sensitivity is improved. Because of the ability of eggplant tissue to eliminate the oxidation of ascorbic acid (AA), the signal of AA is a steady-state response, and thereby the selectivity of the biosensor is enhanced. The effect of experimental conditions such as pH, temperature, ratio of two plant tissues, response time, and linear range were evaluated in order to optimize the analytical performance. The detection limit for DA is 3. 2 × 10⁻⁷ mol/L. Our results showed that the idea of multienzyme system is applicable to the plant tissue-based carbon paste microelectrodes.

The effects of arsenite on activities of several enzymes including mitochondrial pyruvate dehydrogenase (PDH) and succinate dehydrogenase (SDH), microsomal cytochrome P450 and b5, NAD(P)H cytochrome C reductase and glutathione S-transferase were studied. The effects of arsenite on the mitochondrial membrane lipid peroxidation (LPO), the content of hepatic cytosol reduced glutathione and the activity of glutathione peroxidase was also investigated in rats. The results indicated that the activities of mitochondrial PDH and SDH were inhibited to 59 % and 57 % of the control activities respectively after arsenite was administered by intraperitoneal injection (i. p.) for 7 consecutive days at a dose of 20 mg/kg. Administration of arsenite led to a potential decrease of GSH content. The increase in lipid peroxidation of liver mitochondrial membrane prepared from rats treated with arsenite was also observed (P<0. 05). Arsenite did not appear to affect the liver cytosolic glutathione peroxidase and microsomal enzyme activitiesin vivo. Inin vitro test, liver mitochondria and cytosol were treated with arsenite, which led to a decreased SDH activity and GSH content and increase of mitochondrial LPO in a dose-dependent pattern that was similar to the results obtained inin vitro experiments. Selenite played a significant antagonistic role in effects of arsenite eitherin vivo orin vitro on the activities of mitochondrial PDH and SDH, and the content of mitochondrial LPO and cytosolic GSH. This results suggested that the toxic effects of arsenite on rat were associated with increased levels of LPO and the injured SH group in body caused by arsenite.

Intracellular levels of cyclic nucleotide were detected and mitogen stimulation assays were performed in young Sprague-Dawley rats and suspension of spleen tissue were separated by SDS-PAGE. Results indicated that intracellular levels of cyclic AMP in transplanted cell were significantly lower compared to the control group (P<0. 01), the levels of cyclic GMP in transplant cell and the levels of cyclic nucleotide in splenic artery ligation groups were normal. Immunologie tests showed that the stimulation index by Con A for T cells was drastically decreased in the autotransplant and a normal proliferation of B cells after LPS stimulation in transplants. Electrophoresis showed differences in the protein patterns between both tissues. Mitogen stimulation and the protein patterns were not different between the control and splenic artery ligation groups. There were differences between the normal tissues and the transplants at the functional level, Suggesting simple autotransplant can not prevent overwhelming postsplenectomy infection. The intact cellular function after splenic artery ligation indicated that its anti-infection ability is superior to that of splenic transplants.

The effects of phentolamine on hemorrheology and hemodynamics were studied in dogs with acute liver damage induced by acetaminophen. After 1 h of phentolamine application, the viscosity of plasma and whole blood was significantly diminished. The hematocrit readings followed the same pattern as the alterations in viscosity. The portal venous resistance and the value of K were remarkably decreased and the portal venous blood flow was obviously increased. It can be assumed, therefore, that the decrease in viscosity induced by phentolamine results from internal hemodilution and phentolamine may improve hepatic blood circulation through the decrease of portal venous resistance caused by the reduction of blood viscosity and the dilation of portal vascular beds.

Presented in this paper are results of diagnostic and therapeutic radiological intervention for 110 cases of fallopian tube obstruction. Unilateral or bilateral recanalization was achieved in 89 cases, with a success rate being 80. 91 %. Among the 89 patients, patency was achieved by first step treatment (regular HSG) in 40 cases (46 %) and recanalization was successeful in 49 patients (54 %) by sencond and/or third step (selective salpingography plus guide wire). In the 49 cases, 96 occluded tubes were involved, including 89 tubes with proximal obstruction (interstitia-isthmus portion) and 7 tubes with medial-distal obstruction (isthmus-ampulla portion). The success rates for proximal and medial-distal obstruction were 92. 2 % and 7. 8 % respectively. Follow-up results of 45 cases revealed that the tubes of remained patent in 30 cases; 40 became pregnant, among them, 9 gave normal birth of babies; occlusion re-developed in only one case. The authors believe that this radiological intervention can avoid false positive findings frequently occurring with use of regular HSG, thereby enhancing diagnostic accuracy. The selective salpingography could directly increase the intratubal hydrostatic pressure, remove the debris and amiliorate the inflammatory adhersion in the fallopian tubes; selective salpingography plus guide wire recanalization can recanalize the tubes with proximal obstruction with satisfactory results. We recommend the method for the pre-treatment of “test tube baby” procedure.

Inflammatory cells infiltrated in glomeruli and proliferating glomerular cells were immunohistochemically studied in 87 renal biopsies from patients with glomerulonephritides (GN) of various types, by using monoclonal antibody to Mac387 and PCNA (proliferative cell nuclear antigen) respectively. The results showed that the expressions of Mac387 and PCNA were generally increased in proliferative GN, compared with non-proliferative GN. The expression of Mac 387 was related to the severity of mesangial cell proliferation determined by light microscopy, but the increase of PCNA expression was found in severely proliferative GN. Although certain relationship between Mac387 and PCNA expression on glomerular cells was observed, it was not statistically significant. Finally, the possible mechanism of glomerular mesangial proliferation was discussed.

Fourty-eight patients (ASA physical status I — I) undergoing selected thoracotomy and pulmonectomy were studied. They were divided into two groups according to different respiratory modes, two-lung ventilation (TLV) and onelung ventilation (OLV) or TLV and OLV with a Bain cyclic system for CPAP on the side of operated lung. P_etCO₂ and PaCO₂ were measured after 30 min TLV, 30 min and 60 min OLV, and repeated TLV (R-TLV) 30 min after pulmonectomy, to evaluate the difference between P_etCO₂ and PaCO₂ in OLV and to observe the effect of abating hypoxemia and discharge of CO₂ in OLV with Bain system. Our results showed that the PaCO₂ and P_etCO₂ in different test groups were normal though the measured values in OLV were slightly higher than that in TLV (P <0. 05), and then they were recovered after R-TLV (P>0. 05). There was no significant difference between group 1. and 2. in OLV (P>0. 05). There was a close correlation between P_etCO₂ and PaCO₂(P<0.05). The differences of the calculated P_(a-et)CO₂ and radio of P_etCO₂/ PaCO₂ in different ventilation modes were not significant. Hypoxemia in OLV was corrected by Bain system, but the discharge of CO₂ was not affected. The results showed that measurement of P_et CO₂ as a non-invasive procedure can be commonly used to monitor OLV.

This paper reports the use of three-dimensional (3-D) transesophageal echocardiography (TEE) in the diagnosis of atrial septal defect. The results displayed that the interatrial septum had integrity in normal persons. The size, shape and position of atrial septal defects could he showed clearly and the type of the defects could be identified. The reconstructed imaging of interatrial septum on 3-D TEE was clear and stereoscopic. The technique is helpful in defining spatial location and extent of atrial septal defects.

Coronary artery fistula (CAF) is a relatively uncommon cardiovascular disease. It is an abnormal communication between a coronary artery and a cardiac chamber, great vessels, or other vascular structures. Most are congenital. Multiplane transesophageal echocardiography (multiplane TEE, MTEE) can reveal the proximal dilation, the course and the drainage site of CAF. Presented in this paper was a report of right coronary artery-left ventricle fistula, for the first time, right coronary-left ventricle fistula diagnosed by MTEE and confirmed by angiography.

The activity of plasma platelet-activating factor (PAF) from pregnant women before and after delivery was determined. Plasma samples were taken from 74 pregnant women, among whom 24 were normotensive controls, 30 mild and moderate hypertensive and 20 severe hypertensive. Of the two hypertensive groups (pregnancy-induced hypertension, PIH), PAF activity measured by a bioassay was significantly higher than that of normotensive control at 38 weeks in gestation, indicating a possible role of this potent lipid mediator in the pathophysiological mechanism of PIH. After delivery, PAF activity was obviously increased in all three groups, showing the regulation of placenta in PAF metabolism.