Alteration of levels of cyclic nucleotide and analysis of SDS-PAGE in autologous transplanted spleen and splenic artery ligation

Abstract

Intracellular levels of cyclic nucleotide were detected and mitogen stimulation assays were performed in young Sprague-Dawley rats and suspension of spleen tissue were separated by SDS-PAGE. Results indicated that intracellular levels of cyclic AMP in transplanted cell were significantly lower compared to the control group (P<0. 01), the levels of cyclic GMP in transplant cell and the levels of cyclic nucleotide in splenic artery ligation groups were normal. Immunologie tests showed that the stimulation index by Con A for T cells was drastically decreased in the autotransplant and a normal proliferation of B cells after LPS stimulation in transplants. Electrophoresis showed differences in the protein patterns between both tissues. Mitogen stimulation and the protein patterns were not different between the control and splenic artery ligation groups. There were differences between the normal tissues and the transplants at the functional level, Suggesting simple autotransplant can not prevent overwhelming postsplenectomy infection. The intact cellular function after splenic artery ligation indicated that its anti-infection ability is superior to that of splenic transplants.

Keywords

autotransplantation / splenic artery ligation / cyclic nucleotide / mitogen stimutation / SDS-PAGE

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