A new two plant tissue-based carbon paste microelectrode for neurotransmitter detection

Abstract

Presented in this paper is a biosensor constructed for the determination of neurotransmitters. It is an eggplant-banana-tissue-based carbon paste microelectrode. As oxidative enzymes in banana tissue can bio-catalytically oxidize dopamine (DA), norepinephrine (NE), L-dopa, etc., the biosensor’s sensitivity is improved. Because of the ability of eggplant tissue to eliminate the oxidation of ascorbic acid (AA), the signal of AA is a steady-state response, and thereby the selectivity of the biosensor is enhanced. The effect of experimental conditions such as pH, temperature, ratio of two plant tissues, response time, and linear range were evaluated in order to optimize the analytical performance. The detection limit for DA is 3. 2 × 10⁻⁷ mol/L. Our results showed that the idea of multienzyme system is applicable to the plant tissue-based carbon paste microelectrodes.

Keywords

multiple tissue-based carbon paste microelectrode / bio-microelectrode / dopamine / ascorbic acid

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