Self-assembled monolayer of lipoic acid on gold and its application to rapid determination of 2,3,7,8-tetrachlorodibenzo-p-dioxin

Abstract

Lipoic acid (LA) has received great attention in the area of gold surface functionalization. In this study, LA was employed as a linker for the attachment of antibody to the gold surface of surface plasmon resonance (SPR) chip. By using this chip in a homemade SPR immunosensor, low molecular weight compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) can be detected at a low level of 0.01 ng/mL. There is a good linear relationship (R ²=0.943 1) between the results of SPR biosensor and enzyme-linked immunosorbent assay(ELISA).

Keywords

lipoic acid / self-assembled monolayer / 2,3,7,8-TCDD

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