Determination of vitamins B2, B3, B6 and B7 in corn steep liquor by NIR and PLSR

Abstract

Corn steep liquor (CSL) is an important raw material that has high nutritional value and serves as a nitrogen source. Biotin in CSL is especially of great importance to fermentation. In order to develop a fast, versatile, cheap, and environmentally safe analytical method for quantifying vitamins B2 (VB2), B3 (VB3), B6 (VB6) and B7 (VB7) in CSL, the near-infrared spectroscopy (NIR) measurements of 66 samples (22 batches) of CSL were analyzed by partial least-square regression (PLSR). Multivariate models developed in the NIR regions showed good predictive abilities for VB2, VB3, VB6 and VB7. Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.

Keywords

corn steep liquor / NIR / riboflavin / nicotinic acid / pyridoxine / biotin / multivariate calibration / PLSR

References

[1] Edwinoliver N. G., Thirunavukarasu K., Purushothaman S., et al. Corn steep liquor as a nutrition adjunct for the production of Aspergillus niger lipase and hydrolysis of oils thereof [J]. J Agric Food Chem, 2009, 57(22): 10658-10663.

[2] Nascimento R. P., Junior N. A., Pereira N. Jr et al. Brewer’s spent grain and corn steep liquor as substrates for cellulolytic enzymes production by Streptomyces malaysiensis [J]. Lett Appl Microbiol, 2009, 48(5): 529-535.

[3] Chiani M., Akbarzadeh A., Farhangi A., et al. Production of desferrioxamine B (Desferal) using corn steep liquor in Streptomyces pilosus [J]. Pakistan J Bio Sci, 2010, 13(23): 1151-1155.

[4] Gao Y., Yuan Y. J. Comprehensive quality evaluation of corn steep liquor in 2-keto-L-gulonic acid fermentation [J]. J Agric Food Chem, 2011, 59(18): 9845-9853.

[5] Wang L L, Yi H, Wang R G et al. Microorganism growing method for detecting biotin content [J]. China Condiment, 2008(2): 58–60 (in Chinese).

[6] Huang Q., Ding A. Z., Song J. Determination of content of biotin in fermentation liquid by HPLC [J]. Chem Bioeng, 2009, 26(8): 88-90.

[7] Dong Y. G., Wang L., Liu L. D., et al. Extraction and determination of vitamin B2 in millet [J]. Food Sci, 2010, 31(24): 341-344.

[8] Jacobsen J. Optimization of the determination of thiamin, 2-(1-hydroxyethyl) thiamin, and riboflavin in food samples by use of HPLC [J]. Food Chem, 2008, 106(3): 1209-1217.

[9] Ciulu M., Solinas S., Floris I., et al. RP-HPLC determination of water-soluble vitamins in honey [J]. Talanta, 2011, 83(3): 924-929.

[10] Mario A., Gertrud M. Simultaneous determination of riboflavin, pyridoxine, nicotinamide, caffeine and taurine in energy drinks by planar chromatography-multiple detection with confirmation by electrospray ionization mass spectrometry [J]. J Chromatogr A, 2006, 1131(1/2): 253-260.

[11] Mona E. M. M., Amani M. D. E. A. Production of β-mannanase by Bacillus amyloliequifaciens 10A1 cultured on potato peels [J]. Afr J Biotechnol, 2008, 7(8): 1123-1128.

[12] Vendruscolo F., Albuquerque P. M., Streit F., et al. Apple pomace: A versatile substrate for biotechnological applications [J]. Crit Rev Biotechnol, 2008, 28(1): 1-12.

[13] Haake C., Landgrebe D., Scheper T., et al. Online-Infrarotspektroskopie in der Bioprozess analytik[J]. Chem Ing Tech, 2009, 81(9): 1385-1396.

[14] Bureau S., Ruiz D., Reich M., et al. Application of ATRFTIR for a rapid and simultaneous determination of sugars and organic acids in apricot fruit [J]. Food Chem, 2009, 115(3): 1133-1140.

[15] Collell C., Gou P., Arnau J., et al. Non-destructive estimation of moisture, water activity and NaCl at ham surface during resting and drying using NIR spectroscopy [J]. Food Chem, 2011, 129(2): 601-607.

[16] Balabin R. M., Smirnov S. V. Melamine detection by midand near-infrared (MIR/NIR) spectroscopy: A quick and sensitive method for dairy products analysis including liquid milk, infant formula, and milk powder [J]. Talanta, 2011, 85(1): 562-568.

[17] Marietta F., Agnes W., Sandor T., et al. Development of a rapid, non-destructive method for egg content determination in dry pasta using FT-NIR technique [J]. J Food Eng, 2011, 107(2): 195-199.

[18] Maeland A., Sandnes K. Determination of biotin in lowtemperature fish-meal processed from different species by means of a microbiological method using Lactobacillus plantarum as test organism [J]. J Sci Food Agric, 1999, 79(10): 1298-1300.

[19] Qin F., Yang H. H., Lü L. A., et al. Application of near infrared spectra coupled with LLE-PLS modeling to extraction process of Anshen Bunao Syrup [J]. Chin Tradit Patent Med, 2008, 30(10): 1465-1468.

[20] Yang H. H., Qin F., Shen Y. X., et al. Kisomap-PLS nonlinear modeling method and its application in NIR spectral analysis [J]. Spectrosc Spect Anal, 2008, 28(10): 149-150.

[21] Mary D. Q., Gilvan W., Gilcelia A. C., et al. Determination of simple sugars, malic acid and total phenolic compounds in apple pomace by infrared spectroscopy and PLSR [J]. International J Food Sci Tech, 2010, 45(3): 602-609.

[22] Wu W., Massart D. L., Jong S. D. Kernel-PCA algorithms for wide data part II: Fast cross-validation and application in classification of NIR data [J]. Chemometr Intell Lab, 1997, 37(2): 271-280.

[23] Chu X. L., Xu Y. P., Tian G. Y. Practical Guide to Interpretive Near-Infrared Spectroscopy [M]. 2009, Beijing: Chemical Industry Press 46-54.