This review article examines the innovative incorporation of microbeads into hydrogels for wound healing applications, addressing challenges such as infection, scarring, and delayed recovery. Hydrogels are highlighted as effective wound dressings for tissue regeneration, while microbeads serve as versatile carriers for controlled drug release and targeted delivery, allowing customization for specific therapeutic needs. This article explores various preparation methods and materials for microbeads, emphasizing their role in enhancing the antibacterial properties and drug delivery functions of hydrogels. Key properties of microbeads-assisted hydrogels, including biodegradability, biocompatibility, and mechanical strength, are discussed as essential for effective wound management. The integration of microbeads improves drug delivery, antibacterial effects, and tissue regeneration capabilities, providing multifunctional solutions for wound healing. Additionally, the mechanisms of antibacterial agent release are described, focusing on controlled and sustained delivery to wound sites. Applications of microbeads-assisted hydrogels cover drug delivery, antibacterial action, tissue regeneration, and sustained release of growth factors, addressing various wound healing challenges. This article concludes by highlighting significant advancements in wound healing strategies facilitated by the integration of microbeads, promising enhanced therapeutic outcomes for patients.