Histogram analysis of laser speckle contrast image for cerebral blood flow monitoring

Abstract

Laser speckle contrast imaging (LSCI) is a powerful tool for blood flow mapping. In this paper, we described a simple algorithm based on histogram analysis of laser speckle contrast image to provide rapid differentiation between macro- and microcirculations. The algorithm was successfully verified by the study of blood flow in rat cortex under functional activation.

Graphical abstract

Keywords

laser speckle contrast imaging (LSCI) / histogram analysis / cerebral blood flow (CBF) / rat

Full size|PPT slide

Arkady S. Abdurashitov graduated from high school №3 of Saratov. In present time, he is a student of National Research Saratov State University. He is the winner of the Student Scientific Conference, National Research Saratov State University, 2014 and participated in the poster session of Saratov Fall Meeting 2014 conference. His fields of interest are optics, physics, programming, data processing, data visualization.

Full size|PPT slide

Vladislav V. Lychagov received his M.S. degree in Physics in 2003 and Ph.D. degree in Optics in 2007, both from Saratov State University, Russia. Since that time, he obtained his postdoctoral training in Optics and Biophotonics department of Saratov State University. Since 2010, he is research associate professor of Optics and Biophotonics department. His research interests include wave and coherent optics, coherent imaging and microscopy, interferometric measurements and data processing.

Full size|PPT slide

Olga A. Sindeeva received her M.S. degree in Biology (diploma with excellence) in 2012 from Saratov State University, Russia. Since that time, she is a Ph.D. student in Saratov State University, Biological department, Chair of Physiology Human and Animals. Since 2014, she is research associate of Biological department. Her research interests include stress-induced vascular diseases, such as: stroke at different ages and sexes, hypertension, gastric ulcer; stress-limiting system, early indicators of vascular complications.

Full size|PPT slide

Oxana V. Semaychkina-Glushkovskaya received her M.Sc. equivalent in Physiology (diploma with excellence) in 1999, and Ph.D. equivalent in Biological Sciences in 2002, both from Saratov State University, Russia. From 2002 to 2013, she was an Assistant of Professor (2002−2007), Associate Professor (2007−2012), Professor (2012−2013) in Saratov State University, Department of Biology, Chair of Physiology Human and Animals. Since 2013, she is Head of Chair of Physiology of Human and Animals, Department of Biology, Saratov State University. Her research interests include stress-induced vascular damages, stress-limiting system, early indicators of vascular complications, mechanisms underlying neonatal stroke, transformation of peptic ulcer to cancer, role of sex hormones in stress-resistance and stress-reactivity of vascular system, development new animal models: stress-induced neonatal stroke, stress-related peptic ulcer and gastric cancer, technologies for a prognosis of stress-related vascular “catastrophes” such as stroke and ulcer bleeding.

Full size|PPT slide

Valery V. Tuchin received a M.S. degree in Radio-Physics and Electronics (1966), a Ph.D. degree in Optics (1974), and a DrSc in Laser Physics (1982) from Saratov State University, Saratov, Russia. Currently, he is a Professor and holds the Chair of Optics and Biophotonics of Saratov State University. He is also a Director of the Research-Educational Institute of Optics and Biophotonics at Saratov State University and Head of Laboratory on Laser Diagnostics of Technical and Living Systems, Inst. of Precise Mechanics and Control, RAS. His research interests include biophotonics, tissue optics, laser medicine, tissue optical clearing, and nanobiophotonics. He has authored more than 350 peer-reviewed papers, handbooks, monographs, text books, tutorials, and book chapters, holder of more than 50 patents. He is a member of SPIE, OSA, and IEEE. He is a fellow of SPIE and has been awarded Honored Science Worker of the Russia (1999), SPIE Educator Award (2007), FiDiPro (Finland) (2011), and Chime Bell Prize of Hubei Province, China (2014).

References

[1] Fercher A F, Briers J D. Flow visualization by means of single-exposure speckle photography. Optics Communications, 1981, 37(5): 326–330

[2] Li P, Ni S, Zhang L, Zeng S, Luo Q. Imaging cerebral blood flow through the intact rat skull with temporal laser speckle imaging. Optics Letters, 2006, 31(12): 1824–1826

[3] Luo Z, Yuan Z, Pan Y, Du C. Simultaneous imaging of cortical hemodynamics and blood oxygenation change during cerebral ischemia using dual-wavelength laser speckle contrast imaging. Optics Letters, 2009, 34(9): 1480–1482

[4] Dunn A K. Laser speckle contrast imaging of cerebral blood flow. Annals of Biomedical Engineering, 2012, 40(2): 367–377

[5] Parthasarathy A B, Weber E L, Richards L M, Fox D J, Dunn A K. Laser speckle contrast imaging of cerebral blood flow in humans during neurosurgery: a pilot clinical study. Journal of Biomedical Optics, 2010, 15(6): 066030

[6] Briers J D. Laser doppler and time-varying speckle: a reconciliation. Journal of the Optical Society of America A, 1996, 13(2): 345–350

[7] Boas D A, Dunn A K. Laser speckle contrast imaging in biomedical optics. Journal of Biomedical Optics, 2010, 15(1): 011109

[8] Huang Y C, Ringold T L, Nelson J S, Choi B. Noninvasive blood flow imaging for real-time feedback during laser therapy of port wine stain birthmarks. Lasers in Surgery and Medicine, 2008, 40(3): 167–173

[9] Tamaki Y, Araie M, Kawamoto E, Eguchi S, Fujii H. Noncontact, two-dimensional measurement of retinal microcirculation using laser speckle phenomenon. Investigative Ophthalmology & Visual Science, 1994, 35(11): 3825–3834

[10] Sugiyama T, Mashima Y, Yoshioka Y, Oku H, Ikeda T. Effect of unoprostone on topographic and blood flow changes in the ischemic optic nerve head of rabbits. Archives of Ophthalmology, 2009, 127(4): 454–459

[11] Wang J, Zhang Y, Xu T H, Luo Q M, Zhu D. An innovative transparent cranial window based on skull optical clearing. Laser Physics Letters, 2012, 9(6): 469–473

[12] Miao P, Lu H, Liu Q, Li Y, Tong S. Laser speckle contrast imaging of cerebral blood flow in freely moving animals. Journal of Biomedical Optics, 2011, 16(9): 090502 -1–090502-3

[13] Dunn A K, Devor A, Bolay H, Andermann M L, Moskowitz M A, Dale A M, Boas D A. Simultaneous imaging of total cerebral hemoglobin concentration, oxygenation, and blood flow during functional activation. Optics Letters, 2003, 28(1): 28–30

[14] Durduran T, Burnett M G, Yu G, Zhou C, Furuya D, Yodh A G, Detre J A, Greenberg J H. Spatiotemporal quantification of cerebral blood flow during functional activation in rat somatosensory cortex using laser-speckle flowmetry. Journal of Cerebral Blood Flow and Metabolism, 2004, 24(5): 518–525

[15] Dunn A K, Bolay H, Moskowitz M A, Boas D A. Dynamic imaging of cerebral blood flow using laser speckle. Journal of Cerebral Blood Flow and Metabolism, 2001, 21(3): 195–201

[16] Jnguyen. Neuroimaging, 2012

[17] Strong A J, Bezzina E L, Anderson P J, Boutelle M G, Hopwood S E, Dunn A K. Evaluation of laser speckle flowmetry for imaging cortical perfusion in experimental stroke studies: quantitation of perfusion and detection of peri-infarct depolarisations. Journal of Cerebral Blood Flow and Metabolism, 2006, 26(5): 645–653

[18] Bandyopadhyay R, Gittings A S, Suh S S, Dixon P K, Durian D J. Speckle-visibility spectroscopy: a tool to study time-varying dynamics. Review of Scientific Instruments, 2005, 76(9): 093110

[19] Davis M A, Kazmi S M, Dunn A K. Imaging depth and multiple scattering in laser speckle contrast imaging. Journal of Biomedical Optics, 2014, 19(8): 086001

[20] Lemieux P A, Durian D J. Investigating non-Gaussian scattering processes by using nth-order intensity correlation functions. Journal of the Optical Society of America A, 1999, 16(7): 1651–1664

[21] Duncan D D, Kirkpatrick S J. Can laser speckle flowmetry be made a quantitative tool? Journal of the Optical Society of America A, 2008, 25(8): 2088–2094

[22] Ramirez-San-Juan J C, Ramos-García R, Guizar-Iturbide I, Martínez-Niconoff G, Choi B. Impact of velocity distribution assumption on simplified laser speckle imaging equation. Optics Express, 2008, 16(5): 3197–3203

[23] Yuan S, Dunn A K, Boas D A. Calibration in laser speckle contrast imaging. In: Proceedings of Biomedical Topical Meeting Fort Lauderdale, Poster Session II (ME), 2006

[24] Thompson O B, Andrews M K. Tissue perfusion measurements: multiple-exposure laser speckle analysis generates laser Doppler-like spectra. Journal of Biomedical Optics, 2010, 15(2): 027015

[25] Domoki F, Zölei D, Oláh O, Tóth-Szuki V, Hopp B, Bari F, Smausz T. Evaluation of laser-speckle contrast image analysis techniques in the cortical microcirculation of piglets. Microvascular Research, 2012, 83(3): 311–317

[26] Gonzalez R C, Woods R E. Digital Image Processing. New Jersey: Prentice Hall, 2002, 793

[27] Tom W J, Ponticorvo A, Dunn A K. Efficient processing of laser speckle contrast images. IEEE Transactions on Medical Imaging, 2008, 27(12): 1728–1738

[28] Qureshi A I. The importance of acute hypertensive response in ICH. Stroke, 2013, 44(6, Supplement 1): S67–S69

[29] Qureshi A I. Acute hypertensive response in patients with stroke: pathophysiology and management. Circulation, 2008, 118(2): 176–187

[30] Lassen N A. Cerebral blood flow and oxygen consumption in man. Physiological Reviews, 1959, 39(2): 183–238

[31] Heistad D D, Kontos H A. Cerebral circulation. Comprehensive Physiology, 1983, 137–182

[32] Olesen J. The effect of intracarotid epinephrine, norepinephrine, and angiotensin on the regional cerebral blood flow in man. Neurology, 1972, 22(9): 978–987

[33] Semyachkina-Glushkovskaya O V, Lychagov V V, Bibikova O A, Semyachkin-Gluskovskiy I A, Sindeev S S, Zinchenko E M, Kassim M M, Braun H A, Al-Fatle F, Al Hassani L, Tuchin V V. The assessment of pathological changes in cerebral blood flow in hypertensive rats with stress-induced intracranial hemorrhage using Doppler OCT: particularities of arterial and venous alterations. Photonics and Lasers in Medicine, 2013, 2(2): 109–116

Acknowledgement

This work was supported by Russian Science Foundation (No. 14-15-0028).

RIGHTS & PERMISSIONS

2015 Higher Education Press and Springer-Verlag Berlin Heidelberg