RESEARCH ARTCILE

Administration with Bushenkangshuai Tang alleviates UV irradiation- and oxidative stress-induced lifespan defects in nematode Caenorhabditis elegans

  • Qi RUI 1 ,
  • Qin LU 2 ,
  • Dayong WANG , 1
Expand
  • 1. Key Laboratory of Developmental Genes and Human Diseases under the Ministry of Education, Department of Genetics and Developmental Biology, Southeast University Medical School, Nanjing 210009, China
  • 2. Nanjing Maternity and Child Health Hospital, Nanjing 210004, China

Received date: 12 Oct 2008

Accepted date: 02 Nov 2008

Published date: 05 Mar 2009

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

During normal metabolism, oxidative byproducts will inevitably generate and damage molecules thereby impairing their biological functions, including the aging process. Bushenkangshuai Tang (补肾抗衰方, BT) is a traditional Chinese medicine widely used for clinically treating premature ovarian failure. In the present study, BT administration at high concentrations significantly increased lifespan, slowed aging-related decline, and delayed accumulation of aging-related cellular damage in wild-type Caenorhabditis elegans. BT administration could further largely alleviate the aging defects induced by UV and oxidative stresses, and BT administration at different concentrations could largely rescue the aging defects in mev-1 mutant animals. The protective effects of BT administration on aging process were at least partially dependent on the Ins/IGF-like signaling pathway. Moreover, BT administration at different concentrations obviously altered the expression patterns of antioxidant genes and suppressed the severe stress responses induced by UV and oxidative stresses, suggesting that BT-induced tolerance to UV or oxidative stress might result from reactive oxygen species scavenging. BT administration during development was not necessarily a requirement for UV and oxidative stress resistance, and the concentrations of administrated BT examined were not toxic for nematodes. Therefore, BT administration could effectively retrieve the aging defects induced by UV irradiation and oxidative stress in Caenorhabditis elegans.

Cite this article

Qi RUI , Qin LU , Dayong WANG . Administration with Bushenkangshuai Tang alleviates UV irradiation- and oxidative stress-induced lifespan defects in nematode Caenorhabditis elegans[J]. Frontiers of Medicine, 2009 , 3(1) : 76 -90 . DOI: 10.1007/s11684-009-0002-0

Acknowledgements

Strains used in this work were provided by the Caenorhabdits Genetics Center (funded by the National Institutes of Health, National Center for Foundation from Research Resource). This work was supported by the grants from the National Natural Science Foundation of China (Grant No. 30771113), the Program for New Century Excellent Talents in University, and the Nanjing Scientific and Technical Plan (No. 200701113).
1
KlassM R. Aging in nematode Caenorhabditis elegans — major biological and environmental-factors influencing life-span. Mech Ageing Dev, 1977, 6(6): 413-429

DOI

2
BraeckmanB P, VanfleterenJ R. Genetic control of longevity in C. elegans. Exp Gerontol, 2007, 42(1-2): 90-98

DOI

3
ShenL L, WangY, WangD Y. Involvement of genes required for synaptic function in aging control in C. elegans. Neurosci Bull, 2007, 23(1): 21-29

DOI

4
KenyonC, ChangJ, GenschE, RudnerA, TabtiangR A. C. elegans mutant that lives twice as long as wild-type. Nature, 1993, 366(6454): 461-464

DOI

5
KimuraK D, TissenbaumH A, LiuY, RuvkunG. daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. Science, 1997, 277(5328): 942-946

DOI

6
LinK, HsinH, LibinaN, KenyonC. Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling. Nat Genet, 2001, 28(2): 139-145

DOI

7
HarmanD. Aging: a theory based on free radical and radiation chemistry. J Gerontol, 1956, 11(3): 298-300

8
SampayoJ N, OlsenA, LithgowG J. Oxidative stress in Caenorhabditis elegans: protective effects of superoxide dismutase/catalase mimetics. Aging Cell, 2003, 2(6): 319-326

DOI

9
BrysK, VanfleterenJ R, BraeckmanB P. Testing the rate-of-living/oxidative damage theory of aging in the nematode model Caenorhabditis elegans. Exp Gerontol, 2007, 42(9): 845-851

DOI

10
BeckmanK B, AmesB N. The free radical theory of aging matures. Physiol Rev, 1998, 78(2): 547-581

DOI

11
FinkelT, HolbrookN J. Oxidants, oxidative stress and the biology of ageing. Nature, 2000, 408(6809): 239-247

12
LarsenP L. Aging and resistance to oxidative damage in Caenorhabditis elegans. Proc Natl Acad Sci USA, 1993, 90(19): 8905-8909

DOI

13
VanfleterenJ R. Oxidative stress and ageing in Caenorhabditis elegans. Biochem J, 1993, 292(Pt. 2): 605-608

14
IshiiN, FujiiM, HartmanP S, TsudaM, YasudaK, Senoo-MatsudaN, YanaseS, AyusawaD, SuzukiK. A mutation in succinate dehydrogenase cytochrome b causes oxidative stress and ageing in nematodes. Nature, 1998, 394(6694): 694-697

DOI

15
LithgowG J, WhiteT M, MelovS, JohnsonT E. Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress. Proc Natl Acad Sci USA, 1995, 92(16): 7540-7544

DOI

16
MurakamiS, JohnsonT E. A genetic pathway conferring life extension and resistance to UV stress in Caenorhabditis elegans. Genetics, 1996, 143(3): 1207-1218

17
BarsyteD, LovejoyD A, LithgowG J. Longevity and heavy metal resistance in daf-2 and age-1 long-lived mutants of Caenorhabditis elegans. FASEB J, 2001, 15(3): 627-634

DOI

18
LithgowG J, WalkerG A. Stress resistance as a determinate of C. elegans lifespan. Mech Ageing Dev, 2002, 123(7): 765-771

DOI

19
HarringtonL A, HarleyC B. Effects of vitamin E on lifespan and reproduction in Caenorhabditis elegans. Mech Ageing Dev, 1988, 43(11): 71-78

DOI

20
IshiiN, Senoo-MatsudaN, MiyakeK, YasudaK, IshiiT, HartmanP S, FurakawaS. Coenzyme Q10 can prolong C. elegans lifespan by lowering oxidative stress. Mech Ageing Dev, 2004, 125(1): 41-46

DOI

21
JonassenT, LarsenP L, ClarkeC F. A dietary source of coenzyme Q is essential for growth of long-lived Caenorhabditis elegans clk-1 mutants. Proc Natl Acad Sci USA, 2001, 98(2): 421-426

DOI

22
WuZ, SmithJ V, ParamasivamV, ButkoP, KhanL, CypserJ R, LuoY. Ginkgo biloba extract EGb 761 increases stress resistance and extends lifespan of Caenorhabditis elegans. Cell Mol Biol, 2002, 48(6): 725-731

23
LinY T, HoangH, HsiehS, RangelN, FosterA L, SampayoJ N, LithgowG J, SrinivasanC. Manganous ion supplementation accelerates wild type development, enhances stress resistance, and rescues the life span of a short-lived Caenorhabditis elegans mutant. Free Radical Biol Med, 2006, 40(7): 1185-1193

DOI

24
GoswamiD, ConwayG S. Premature ovarian failure. Hum Reprod Update, 2005, 11(4): 391-410

DOI

25
PalL, SantoroN. Premature ovarian failure (POF): discordance between somatic and reproductive aging. Ageing Res Rev, 2002, 1(3): 413-423

DOI

26
HuX M, RobertsJ R, ApopaP L, KanY W, MaQ. Accelerated ovarian failure induced by 4-vinyl cyclohexene diepoxide in Nrf2 null mice. Mol Cell Biol, 2006, 26(3): 940-954

DOI

27
GraziewiczM A, BienstockR J, CopelandW C. The DNA polymerase γ Y955C disease variant associated with PEO and parkinsonism mediates the incorporation and translesion synthesis opposite7, 8-dihydro-8-oxo-2’-deoxyguanosine. Hum Mol Genet, 2007, 16(22): 2729-2739

DOI

28
BrennerS. The genetics of Caenorhabditis elegans. Genetics, 1974, 77(1): 71-94

29
DonkinS G, WilliamsP L. Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing. Environ Toxicol Chem, 1995, 14(12): 2139-2147

DOI

30
WilliamsP L, DusenberyD B. Aquatic toxicity testing using the nematode Caenorhabditis elegans. Environ Toxicol Chem, 1990, 9(10): 1285-1290

DOI

31
YeH Y, YeB P, WangD Y. Trace administration of vitamin E can retrieve and prevent UV-irradiation- and metal exposure-induced memory deficits in nematode Caenorhabditis elegans. Neurobiol Learn Mem, 2008, 90(1): 10-18

DOI

32
WilsonM A, Shukitt-HaleB, KaltW, IngramD K, JosephJ A, WolkowC A. Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans. Aging Cell, 2006, 5(1): 59-68

DOI

33
WangY, XieW, WangD Y. Transferable properties of multi-biological toxicity caused by cobalt exposure in Caenorhabditis elegans. Environ Toxicol Chem, 2007, 26(11): 2405-2412

DOI

34
HuangC, XiongC, KornfeldK. Measurements of age-related changes of physiological processes that predict lifespan of Caenorhabditis elegans. Proc Natl Acad Sci USA, 2004, 101(21): 8084-8089

DOI

35
WangD Y, WangY. Phenotypic and behavioral defects caused by barium exposure in nematode Caenorhabditis elegans. Arch Environ Contam Toxicol, 2008, 54(3): 447-453

DOI

36
YanaseS, IshiiN. Cloning of the oxidative stress-responsive genes in Caenorhabditis elegans. J Rad Res, 1999, 40(1): 39-47

DOI

37
MorseD P, BassB L. Long RNA hairpins that contain inosine are present in Caenorhabditis elegans poly(A)+ RNA. Proc Natl Acad Sci USA, 1999, 96(11): 6048-6053

DOI

38
GemsD, SuttonA J, SundermeyerM L, AlbertP S, KingK V, EddleyM L, LarsenP L, RiddleD L. Two pleiotropic classes of daf-2 mutations affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans. Genetics, 1998, 150(1): 129-155

39
BrunkU, TermanA. Lipofuscin: mechanisms of age-related accumulation and influence on cell function. Free Radic Biol Med, 2002, 33(5): 611-619

DOI

40
YanaseS, YasudaK, IshiiN. Adaptive responses to oxidative damage in three mutants of Caenorhabditis elegans (age-1, mev-1and daf-16) that affect life span. Mech Ageing Dev, 2002, 123(12): 1579-1587

DOI

41
OttolenghiC, UdaM, HamataniT, CrisponiL, GarciaJ E, KoM, PiliaG, SforzaC, SchlessingerD, ForaboscoA. Aging of oocyte, ovary, and human reproduction. Ann N Y Acad Sci, 2004, 1034: 117-131

DOI

42
RichardsonS J, SenkiasV, NelsonJ F. Follicular depletion during menopausal transition: evidence for accelerated loss and ultimate exhaustion. J Clin Endocrinol Metab, 1987, 65(6): 1231-1237

43
SnowdownD A, KaneR L, BeesonW L, BurkeG L, SprafkaJ M, PotterJ, IsoH, JacobsD R Jr, PhillipsR L. Is early natural menopause a biological marker of health and aging? Am J Public Health, 1989, 79(6): 709-714

DOI

44
AnastiJ N. Premature ovarian failure: an update. Fertil Steril, 1998, 70(1): 1-15

DOI

45
BrambillaD J, McKinleyS M. A prospective study of factors affecting age at menopause. J Clin Epidemiol, 1989, 42(11): 1031-1039

DOI

46
HolzenbergerM, DupontJ, DucosB, LeneuveP, GéloënA, EvenP C, CerveraP, BoucY L. IGF-1 receptor regulates lifespan and resistance to oxidative stress in mice. Nature, 2003, 421(6919): 182-186

DOI

47
WiseP M. Neuroendocrine modulation of the menopause: insights into the aging brain. Am J Physiol (Endocrinol Metab), 1999, 40(6): E965-970

48
YangW, LiJ, HekimiS. A measurable increase in oxidative damage due to reduction in superoxide detoxification fails to shorten the life span of long-lived mitochondrial mutants of Caenorhabditis elegans. Genetics, 2007, 177(4): 2063-2074

DOI

49
CastrillonD H, MiaoL, KolliparaR, HornerJ W, DePinhoR A. Suppression of ovarian follicle activation in mice by the transcription factor Foxo3a. Science, 2003, 301(5630): 215-217

DOI

50
BayneA C, SohalR S. Effects of superoxide dismutase/catalase mimetics on life span and oxidative stress resistance in the housefly, Musca domestica. Free Radic Biol Med, 2002, 32(11): 1229-1234

DOI

51
KeaneyM, GemsD. No increase in lifespan in Caenorhabditis elegans upon treatment with the superoxide dismutase mimetic Euk-8. Free Radic Biol Med, 2003, 34(2): 277-282

DOI

52
HulleyS, GradyD, BushT, FurbergC, HerringtonD, RiggsB, VittinghoffE. Randomized trial of estrogen plus progestin for secondary prevention of coronary heart disease in postmenopausal women. JAMA, 1998, 280(7): 605-613

DOI

53
Letur-KonirschH, DelanianS. Successful pregnancies after combined pentoxifylline-tocopherol treatment in women with premature ovarian failure who are resistant to hormone replacement therapy. Fertil Steril, 2003, 79(2): 439-441

DOI

Outlines

/