Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation by targeting HAND2

Abstract

Congenital heart disease (CHD) is the most common birth defect worldwide. Long non-coding RNAs (lncRNAs) have been implicated in many diseases. However, their involvement in CHD is not well understood. This study aimed to investigate the role of dysregulated lncRNAs in CHD. We used Gene Expression Omnibus data mining, bioinformatics analysis, and analysis of clinical tissue samples and observed that the novel lncRNA SAP30-2:1 with unknown function was significantly downregulated in damaged cardiac tissues from patients with CHD. Knockdown of lncRNA SAP30-2:1 inhibited the proliferation of human embryonic kidney and AC16 cells and decreased the expression of heart and neural crest derivatives expressed 2 (HAND2). Moreover, lncRNA SAP30-2:1 was associated with HAND2 by RNA immunoprecipitation. Overall, these results suggest that lncRNA SAP30-2:1 may be involved in heart development through affecting cell proliferation via targeting HAND2 and may thus represent a novel therapeutic target for CHD.

Keywords

congenital heart disease / Gene Expression Omnibus / lncRNA SAP30-2:1 / cell proliferation / RNA immunoprecipitation / HAND2

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Acknowledgements

This study was supported by grants from the National Key Research and Development Program of China (No. 2016YFC1000500), the National Science Foundation for Young Scientists (No. 81801501), and the Postdoctoral Science Foundation of China (No. 2018M632026).

Compliance with ethics guidelines

Jing Ma, Shiyu Chen, Lili Hao, Wei Sheng, Weicheng Chen, Xiaojing Ma, Bowen Zhang, Duan Ma, and Guoying Huang declare that they have no conflict of interest. All the human tissues used in the present study were obtained with written informed consent. This study was approved by the Institutional Research Ethics Committee of the Children’s Hospital of Fudan University (2016-56).

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11684-020-0778-5 and is accessible for authorized users.

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