Deciphering the pharmacological mechanism of Guan-Jie-Kang in treating rat adjuvant-induced arthritis using omics analysis

Hudan Pan, Yanfang Zheng, Zhongqiu Liu, Zhongwen Yuan, Rutong Ren, Hua Zhou, Ying Xie, Liang Liu

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Front. Med. ›› 2019, Vol. 13 ›› Issue (5) : 564-574. DOI: 10.1007/s11684-018-0676-2
RESEARCH ARTICLE

Deciphering the pharmacological mechanism of Guan-Jie-Kang in treating rat adjuvant-induced arthritis using omics analysis

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Abstract

Traditional Chinese medicine (TCM) formulas have attracted increasing attention worldwide in the past few years for treating complex disease including rheumatoid arthritis. However, their mechanisms are complex and remain unclear. Guan-Jie-Kang (GJK), a prescription modified from “Wu Tou Decoction,” was found to significantly relieve arthritis symptoms in rats with adjuvant-induced arthritis after 30-day treatment, especially in the 24 g/kg/day group. By analyzing 1749 targets related to 358 compounds in the five herbs of GJK, we identified the possible anti-arthritis pathways of GJK, including the calcium signaling and metabolic pathways. Bone damage levels were assessed by micro-computed tomography, and greater bone protective effect was observed with GJK treatment than with methotrexate. Receptor activator of nuclear factor κB ligand (RANKL)–RANK signaling, which is related to calcium signaling, was significantly regulated by GJK. Moreover, a target metabolomics assay of serum was conducted; 17 metabolic biomarkers showed significant correlations with treatment. An integrated pathway analysis revealed that pyruvate metabolism, purine metabolism, and glycolysis metabolism were significantly associated with the effects of GJK in arthritis treatment. Thus, this study establishes a new omics analytical method integrated with bioinformatics analysis for elucidating the multi-pathway mechanisms of TCM.

Keywords

rheumatoid arthritis / traditional Chinese medicine / pharmacological mechanism / metabolism / adjuvant-induced arthritis / omics analysis

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Hudan Pan, Yanfang Zheng, Zhongqiu Liu, Zhongwen Yuan, Rutong Ren, Hua Zhou, Ying Xie, Liang Liu. Deciphering the pharmacological mechanism of Guan-Jie-Kang in treating rat adjuvant-induced arthritis using omics analysis. Front. Med., 2019, 13(5): 564‒574 https://doi.org/10.1007/s11684-018-0676-2

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Acknowledgements

This work was financially supported by grants from the Macau Science and Technology Development Fund (No. 102/2016/A3).

Compliance with ethics guidelines

Hudan Pan, Yanfang Zheng, Zhongqiu Liu, Zhongwen Yuan, Rutong Ren, Hua Zhou, Ying Xie, and Liang Liu declare that they have no conflicts of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.
Electronic Supplementary Material&chsp;ƒSupplementary materials are available in the online version of this article at https://doi.org/10.1007/s11684-018-0676-2 and are accessible for authorized users.

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2019 Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature
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