Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality

Bo Huang, Zhou Li, Xinling Ren, Jihui Ai, Lixia Zhu, Lei Jin

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Front. Med. ›› 2017, Vol. 11 ›› Issue (2) : 247-252. DOI: 10.1007/s11684-017-0519-6
RESEARCH ARTICLE

Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality

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Abstract

The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IV embryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.

Keywords

in vitro fertilization / PCOS / free radical / embryo quality

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Bo Huang, Zhou Li, Xinling Ren, Jihui Ai, Lixia Zhu, Lei Jin. Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality. Front. Med., 2017, 11(2): 247‒252 https://doi.org/10.1007/s11684-017-0519-6

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Acknowledgements

This study was supported by the Chinese Society of Reproductive Medicine (No. 16020520668).

Compliance with ethics guidelines

Bo Huang, Zhou Li, Xinling Ren, Jihui Ai, Lixia Zhu, and Lei Jin declare that they have no conflict of interest. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with theHelsinki Declaration of 1975, as revised in 2000 (). Informed consent was obtained from all patients for being included in the study.

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2017 Higher Education Press and Springer-Verlag Berlin Heidelberg
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