Coronary leukocyte activation in relation to progression of coronary artery disease

Abstract

Leukocyte activation has been linked to atherogenesis, but there is little in vivo evidence for its role in the progression of atherosclerosis. We evaluated the predictive value for progression of coronary artery disease (CAD) of leukocyte activation markers in the coronary circulation. Monocyte and neutrophil CD11b, neutrophil CD66b expression and intracellular neutrophil myeloperoxidase (MPO) in the coronary arteries were determined by flow cytometry in patients undergoing coronary angiography. The primary outcome included fatal and nonfatal myocardial infarction or arterial vascular intervention due to unstable angina pectoris. In total 99 subjects who were included, 70 had CAD at inclusion (26 patients had single-vessel disease, 18 patients had two-vessel disease and 26 patients had three-vessel disease). The median follow-up duration was 2242 days (interquartile range: 2142–2358). During follow-up, 13 patients (13%) developed progression of CAD. Monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO measured in blood obtained from the coronary arteries were not associated with the progression of CAD. These data indicate that coronary monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO do not predict the risk of progression of CAD.

Keywords

coronary artery disease / inflammation / integrin / myeloperoxidase / leukocyte activation

References

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Acknowledgements

This work was fully supported by the Research Foundation Internal Medicine of the Sint Franciscus Gasthuis in Rotterdam, The Netherlands.

Compliance with ethics guidelines

Marijke A. de Vries, Arash Alipour, Erwin Birnie, Andrew Westzaan, Selvetta van Santen, Ellen van der Zwan, Anho H. Liem, Noëlle van der Meulen, and Manuel Castro Cabezas declare that they have no conflict of interest. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000 (5). Informed consent was obtained from all patients for being included in the study.

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