Transcriptomics and proteomics in stem cell research

Abstract

Stem cells are capable of self-renewal and differentiation, and the processes regulating these events are among the most comprehensively investigated topics in life sciences. In particular, the molecular mechanisms of the self-renewal, proliferation, and differentiation of stem cells have been extensively examined. Multi-omics integrative analysis, such as transcriptomics combined with proteomics, is one of the most promising approaches to the systemic investigation of stem cell biology. We reviewed the available information on stem cells by examining published results using transcriptomic and proteomic characterization of the different stem cell processes. Comprehensive understanding of these important processes can only be achieved using a systemic methodology, and employing such method will strengthen the study on stem cell biology and promote the clinical applications of stem cells.

Keywords

embryonic stem cells / transcriptomics / proteomics

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Acknowledgements

The authors thank Dr. Yuxia Jiao for critically reading the manuscript. This research was supported by the “Strategic Priority Research Program” of the Chinese Academy of Sciences, Stem Cell and Regenerative Medicine Research (XDA01040405 to X.F.), National Key Scientific Instrument and Equipment Development Projects of China (2011YQ03013404 to X.F.), National High Technology Research and Development Program of China (863 Program, 2012AA022502 to X.F.).

Compliance with ethics guidelines

Hai Wang, Qian Zhang, and Xiangdong Fang declare that they have no conflict of interest. This manuscript is a review article and does not involve a research protocol requiring approval by the relevant institutional review board or ethics committee.

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