Effect of pirfenidone on renal tubulointerstitial fibrosis

Dixin LI MM , Hongbing ZENG MD , Chunyang JI MM ,

PDF(452 KB)
PDF(452 KB)
Front. Med. ›› 2009, Vol. 3 ›› Issue (3) : 316-322. DOI: 10.1007/s11684-009-0045-2
Research articles

Effect of pirfenidone on renal tubulointerstitial fibrosis

  • Dixin LI MM , Hongbing ZENG MD , Chunyang JI MM ,
Author information +
History +

Abstract

Renal tubulointerstitial fibrosis (TIF) is the common end stage of various chronic renal diseases, and pirfenidone (PFD) is a novel, broad-spectrum anti-fibrotic compound but little is known about its effect and mechanism of action on renal TIF. In this work, we employed a unilateral ureteral obstruction (UUO) rat model to investigate the apoptosis of renal tubular epithelial cells (RTC) after PFD treatment. Thirty-five Sprague Dawley (SD) rats were randomized into three groups: sham-operated group (n=7), UUO group (n=14) and PFD group (n=14). All rats were sacrificed at day 7 or 14 after operation. Renal histology was studied by using periodic acid schiff reagent (PAS) and Masson trichromic stain (MASSON); apoptosis was detected by in situ terminal deoxynucleotide transferase-mediated dUTP-biotin nick end-labeling (TUNEL); tubular caspase-3 expression was assessed by immunohistochemistry. The content of malondialdehyde (MDA) and total activity of superoxide dismutase (T-SOD) in the renal cortex was determined by chemical colorimetry method. TIF, apoptosis of RTC, tubular expression of caspase-3 and the content of MDA were increased in the UUO group compared with those in the sham-operated group, and were ameliorated significantly by PFD treatment (P<0.05). The activity of SOD was decreased in the UUO group, but was improved by PFD treatment (P<0.05). Our results showed that PFD could ameliorate TIF in the UUO group, and the possible mechanism was by reducing the apoptosis of RTC, which involved oxidative stress and caspase-3.

Keywords

pirfenidone / apoptosis / caspase 3 / oxidative stress

Cite this article

Download citation ▾
Dixin LI MM , Hongbing ZENG MD , Chunyang JI MM ,. Effect of pirfenidone on renal tubulointerstitial fibrosis. Front. Med., 2009, 3(3): 316‒322 https://doi.org/10.1007/s11684-009-0045-2
AI Summary AI Mindmap
PDF(452 KB)

Accesses

Citations

Detail

Sections
Recommended

/