Effect of Ca²⁺ complexation on oxygen partial pressure in the urinary bladder meridian of goats

Abstract

The aim of this paper was to explore the relationship between energy metabolism and the meridian phenomenon. The manner of change of oxygen partial pressure in acupoints and no-acupoints of the urinary bladder meridian of goats was observed with a needle-type tissue oxygen tension sensor after the acupuncture effect was blocked by Ca²⁺ complexation with ethylenediamine tetraacetic acid (EDTA)-Na₂. The results showed that: (1) the concentration of calcium ion in urinary bladder meridian acupoints was higher than that in no-acupoints (P<0.01 or P<0.05); (2) the tissue oxygen tension in urinary bladder meridian acupoints was higher than that in no-acupoints (P<0.01); (3) the oxygen partial pressure in acupoints decreased significantly after injection with EDTA-Na₂, compared with injection of either normal sodium or nothing (P<0.01); (4) the oxygen partial pressure in acupoints was higher than that in no-acupoints after EDTA-Na₂ injection (P<0.01). Acupuncture could reduce oxygen partial pressure in acupoints by regulating microcirculation and increasing metabolic levels.

Keywords

oxygen partial pressure / biosensor / calcium channels / acupoint

References

[1] GuoY,ZhangY J,WanY Y,XuT P,MiaoW F,ZhangC X. The effects of complexing the Ca(2+) at zusanli upon inhibiting the motion of stomach of the normal rabbits by acupuncture at Zusanli. Zhenci Yanjiu, 1998, (2): 146-148 (in Chinese)

[2] YuA S,ZhaoY X,YanZ G,LiX L,GuanX F. Space morphology observation of the Sanyinjiao acupoint. Zhongguo Zhenjiu, 1997, (1): 42-44 (in Chinese)

[3] XuX Y,HuX L,WuB H. The influence of mechanical pressing on partial pressure of oxygen in three points along the large intestine meridian during acupuncture. Zhenci Yanjiu, 2000, 25(4): 276-279 (in Chinese)

[4] WangP,ZhangH P,LiuY X. Observation of the partial pressure of oxygen in deep tissue of acupoints, no-acupoints in meridian and no-acupoints. Shanghai Zhenjiu Zazhi, 1997, 16(1): 3-5 (in Chinese)

[5] XieY J,HuX L,WuB H. Observation of the partial pressure of oxygen in deep tissue along the meridian course and the effect of acupuncture on it. Zhongguo Zhongyiyao Keji, 2000, 7(6): 353-354 (in Chinese)

[6] JiangH Z,WangQ,ZhangM M,GongP,HuangG Y. Effect of acupuncture on pH values of acupoints in the goat. Zhongguo Zhenjiu2006, 26(10): 732-734 (in Chinese)

[7] SneydJ,KeizerJ,SandersonM J. Mechanisms of calcium oscillations and waves: a quantitative analysis. FASEB J, 1995, 9(14): 1463-1472

[8] SandersenM J,CharlesA C,DirksenE R. Mechanical stimulation and intercellular communication increased intercellular Ca²⁺ in epithelial cells. Cell Regul, 1990, 1(8): 585-596

[9] ZhengC H,HuangG Y,ZhangM M. Experimental study on connexin43 expression in “Zusnali”(ST 43) with fluorescence dual-label technique in rats. Zhenci Yanjiu, 2005, 30(4): 221-224 (in Chinese)

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Higher Education Press and Springer-Verlag Berlin Heidelberg