Repetitive transcranial magnetic stimulation causes significant changes of chemical substances in the brain of rabbits with experimental intracerebral hemorrhage

Abstract

More and more studies have reported the usefulness of repetitive transcranial magnetic stimulation (rTMS) in the treatment of stroke patients. This article is to explore if rTMS can cause changes of such chemical substances as N-acetylaspartate (NAA), choline (Cho) and creatine (Cr) in the surrounding area of experimental intracerebral hematoma of rabbits. A total of 36 rabbits were randomly divided into 3 groups: a control group (group A), a sham rTMS group (group B) and an rTMS group (group C). The experimental intracerebral hemorrhage (ICH) was induced by intracerebral injection of self-body blood of the animals in groups B and C, while those of the group A serving as controls were injected with normal saline. The rabbits of groups B and C were treated with sham and real rTMS, respectively, but those of group A were not. The contents of chemical substances including NAA, Cho and Cr in the perihematomal brain tissues were measured by using ^lH-magnetic resonance spectroscopy (¹H-MRS) 12 hours, 72 hours, 1 week and 2 weeks after the experimental ICH was induced. The results show that the contents of NAA, Cr and NAA/Cr ratio were decreased significantly, but Cho, Cho/Cr increased significantly in groups B and C (P < 0.01) as compared against those in group A. A comparison between the groups B and C revealed that the contents of NAA, Cr, NAA/Cr were higher, but Cho and Cho/Cr were significantly lower in group C than those in group B (P < 0.01). It is concluded that rTMS could result in changes of the chemical substance contents in perihematomal brain tissues, which leads to neuroprotective effects in the brain.

References

1. Swayne O B C, Rothwell J C, Ward N S, Greenwood R J . Stages of motor output reorganization after hemispheric stroke suggestedby longitudinal studies of cortical physiology. Cereb Cortex, 2008, 18(8): 1909–1922. doi:10.1093/cercor/bhm218
2. Chen R, Cros D, Curra A, Di Lazzaro V, Lefaucheur J P, Magistris M R, Mills K, Rösler K M, Triggs W J, Ugawa Y, Ziemann U . The clinical diagnostic utility of transcranialmagnetic stimulation: report of an IFCN committee. Clin Neurophysiol, 2008, 119(3): 504–532. doi:10.1016/j.clinph.2007.10.014
3. Reis J, Swayne O B, Vandermeeren Y, Camus M, Dimyan M A, Harris-Love M, Perez M A, Ragert P, Rothwell J C, Cohen L G . Contribution of transcranialmagnetic stimulation to the understanding of cortical mechanisms involvedin motor control. J Physiol, 2008, 586(2): 325–351. doi:10.1113/jphysiol.2007.144824
4. O'Reardon J P, Solvason H B, Janicak P G, Sampson S, Isenberg K E, Nahas Z, McDonald W M, Avery D, Fitzgerald P B, Loo C, Demitrack M A, George M S, Sackeim H A . Efficacy and safety of transcranial magnetic stimulation in the acutetreatment of major depression: a multisite randomized controlled trial. Biol Psychiatry, 2007, 62(11): 1208–1216. doi:10.1016/j.biopsych.2007.01.018
5. Kinoshita M, Ikeda A, Begum T, Yamamoto J, Hitomi T, Shibasaki H . Low-frequency repetitive transcranial magnetic stimulationfor seizure suppression in patients with extratemporal lobe epilepsy:a pilot study. Seizure, 2005, 14(6): 387–392
6. Ikeguchi M, Touge T, Nishiyama Y, Takeuchi H, Kuriyama S, Ohkawa M . Effects of successive repetitive transcranial magneticstimulation on motor performances and brain perfusion in idiopathicParkinson's disease. J Neurol Sci, 2003, 209(1–2): 41–46. doi:10.1016/S0022-510X(02)00459-8
7. Rollnik J D, Wüstefeld S, Däuper J, Karst M, Fink M, Kossev A, Dengler R . Repetitive transcranial magneticstimulation for the treatment of chronic pain–a pilot study. Eur Neurol, 2002, 48(1): 6–10. doi:10.1159/000064950
8. Niu Z, Zhang T, Fang D . The effect of TMS on motor function in patients withacute brain infarction. Zhongguo KangfuLilun Yu Shijian, 2001, 7(1): 16–18 (in Chinese)
9. Jin X, Wu X, Wang J, Huang B, Wang Q, Zhang T, Niu Z, Zhang X . Effect of transcranial magnetic stimulation on rehabilitationof motor function in patients with cerebral infarction. Zhonghua Yi Xue Za Zhi, 2002, 82(8): 534–553 (in Chinese)
10. Zhao H, Sun Y, Dai Y . The effects of TMS on BDNF expression and infarct volumein rats with cerebral ischemia. ZhonghuaShenjingbingxue Zazhi, 2005, 38 (5): 330–331 (in Chinese)
11. Sun Y, Zao H, Zhang Z, Huo H, Li W, Bao S . Effect of long-termtranscranial magnetic stimulation on the expression of brain-derivedneurotrophic factor in cortex around the infarcted focus and the recoveryof neurologic function in rats with cerebral infarction. Zhonghua Wuli Yixue Yu Kangfu Zazhi, 2005, 27(12): 712–716 (in Chinese)
12. Wang T C, Guo T C, Zhu X L . An experimental study of the effect of rTMS on cerebralhemorrhage in rabbits. Zhonghua Wuli YixueYu Kangfu Zazhi, 2003, 25: 390–393 (in Chinese)
13. Guo T C, Cao X B, Zhang Y J, Sun S G, Zhu X L . Effect of rTMS on contents of monoamineand amino acid transmitters in rabbits with cerebral hemorrhage. Zhonghua Wuli Yixue Yu Kangfu Zazhi, 2004, 26(8): 452–455 (in Chinese)
14. Barker P B, Gillard J H, van Zijl P C, Soher B J, Hanley D F, Agildere A M, Oppenheimer S M, Bryan R N . Acute stroke: evaluation with serial proton MR spectroscopicimaging. Radiology, 1994, 192(3): 723–732
15. Auld K L, Ashwal S, Holshouser B A, Tomasi L G, Perkin R M, Ross B D, Hinshaw D B Jr . Proton magnetic resonancespectroscopy in children with acute central nervous system injury. Pediatr Neurol, 1995, 12(4): 323–334. doi:10.1016/0887-8994(95)00062-K
16. Lanfermann H, Kugel H, Heindel W, Herholz K, Heiss W D, Lackner K . Metabolic changes in acute and subacute cerebral infarctions:findings at proton MR spectroscopic imaging. Radiology, 1995, 196(1): 203–210
17. Peng H, Li Y F, Sun S G . Effects of Ginkgo biloba extract on acute cerebral ischemiain rats analyzed by magnetic resonance spectroscopy. Acta Pharmacol Sin, 2003, 24(5): 467–471
18. Handa Y, Kaneko M, Matuda T, Kobayashi H, Kubota T . Invivo proton magnetic resonance spectroscopy for metabolicchanges in brain during chronic cerebral vasospasm in primates. Neurosurgery, 1997, 40(4): 773–180. doi:10.1097/00006123-199704000-00023
19. Kamada K . Sequentialobservations of brain edema with proton magnetic resonance imagingand spectroscopy. Hokkaido Igaku Zasshi, 1996, 71(1): 105–122
20. Ye C L, Yu M K, Jiang J Y, Zhang G J, Lu Y C, Zhu C . ¹H Magnetic resonance spectroscopy analysis of frontallobe in patients with traumatic brain injury. Zhonghua Yi Xue Za Zhi, 2003, 83(2): 140–141 (in Chinese)