Label-free colorimetric nanosensor with improved sensitivity for Pb²⁺ in water by using a truncated 8–17 DNAzyme

Highlights

• Unmodified-AuNP based, colorimetric nanosensor was constructed for Pb²⁺ detection.

• 5-nucleotide truncation in DNAzyme made complete substrate detachment upon Pb²⁺.

• Ultrasensitive and selective detection of Lead (II) was achieved with 0.2×10^-9 mol/L LOD.

Abstract

Water pollution accidents, such as the Flint water crisis in the United States, caused by lead contamination have raised concern on the safety of drinking water distribution systems. Thus, the routine monitoring of lead in water is highly required and demands efficient, sensitive, cost-effective, and reliable lead detection methods. This study reports a label-free colorimetric nanosensor that uses unmodified gold nanoparticles (AuNPs) as indicators to enable rapid and ultra-sensitive detection of lead in environmental water. The 8–17 DNAzyme was truncated in this study to facilitate the detachment of single-stranded DNA fragments after substrate cleavage in the presence of Pb²⁺. The detached fragments were adsorbed over AuNPs and protected against salt concentration-induced aggregation. Accordingly, high Pb²⁺ would result in rapid color change from blue to pink. The established sensing principle achieved a sensitive limit of detection of 0.2×10^-9 mol/L Pb²⁺, with a linear working range of two orders of magnitude from 0.5×10^-9 mol/L to 5×10^-9 mol/L. The selectivity of the nanosensor was demonstrated by evaluating the interfering metal ions. The developed nanosensor can serve as a substitute for the rapid analysis and monitoring of trace lead levels under the drinking water distribution system and even other environmental water samples.

Graphical abstract

Keywords

Colorimetric nanosensor / Truncated 8–17 DNAzyme / Pb²⁺ detection / Unmodified AuNPs

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Acknowledgements

This research is supported by a Special fund of State Key Joint Laboratory of Environment Simulation and Pollution Control (No. 16Y04ESPCT), and the Major Scientific Equipment Development Project of China (No. 2012YQ030111).

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-019-1094-7 and is accessible for authorized users.

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