Single and combined effects of secondary polyethylene microplastic on the growth of Pak choi and the soil microbiome composition

Abstract

● Secondary PE-MPs were simulated via the aging processes and mechanical milling.

● The growth of Pak choi was greatly inhibited after secondary PE-MPs exposure.

● Combined effects of secondary PE-MPs and pollutants were antagonism.

● Soil properties and microbial composition showed significant alteration.

It has been confirmed that microplastics (MPs) are present in the environment. This study simulated secondary PE-MPs via aging and mechanical processes to evaluate their effects on Pak choi (Brassica rapa L.) over 21 d. Two common pollutants, dichlorodiphenyltrichloroethane (DDT) and naphthalene, were used in the combined toxicity tests. The results indicated that the growth of Pak choi was significantly inhibited after exposure to secondary PE-MPs, and the combined effects were antagonistic, owing to the adsorption capacity of secondary PE-MPs to DDT and naphthalene. Oxidative stress in Pak choi can be markedly affected, leading to oxidative damage to plant cells. The moisture content, soil bulk density, soil density, cation exchange capacity (CEC), and FDA hydrolase in the planted soils increased in the treated groups, and the TOC content changed significantly. We also found that the microbial composition of the soil in the DDT and naphthalene groups showed more significant alterations than that in the other groups. Alpha diversity analysis showed that species diversity increased in the combined groups but indicated a clear downward trend in the single MPs groups. This study suggests that secondary PE-MPs harm the growth of Pak choi and can change soil properties, revealing the harm to the ecosystem of MPs in the soil.

Graphical abstract

Keywords

Secondary PE-MPs / Combined effects / Oxidative stress / Soil properties / Bacterial community

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