Comparison of exogenous degrader-enhanced bioremediation with low-dose persulfate oxidation for polycyclic aromatic hydrocarbon removal in alkaline soil: efficiency and influence on ecological health

Highlights

● Bioaugmentation and low-dose persulfate were effective in degrading PAHs.

● Indigenous microorganisms participated in the degradation process.

● Low-dose persulfate oxidation made a high activated phosphorus content.

● Low microbial species diversity made microbial system weak in BA system.

Abstract

Polycyclic aromatic hydrocarbon (PAH)-contaminated soils are usually complex and characterized by a lack of nutrition and soil salinization, resulting in difficulties in soil remediation. In this study, bioaugmentation with a PAH-degrading Bacillus PheN7 (BA) and low-dose persulfate oxidation (PS), along with natural biodegradation, were utilized to remediate alkaline PAH-contaminated soil. The soil used in the study had a pH of 9.35, and the total PAH content was 568.8 ± 31.0 mg/kg dry soil. After 42 d of remediation, the degradation efficiency of PAHs was 96.72% and 93.88% using persulfate oxidation and bioaugmentation, respectively, whereas 38.66% of PAHs were degraded in natural attenuation (NA). Bacillus was the dominant genera throughout the process of bioremediation with the relative abundance of 79.3% on day 42 in the BA system, whereas, Alcanivorax was enriched and became the dominant genera in PS systems. In the meantime, PAH degradation genes were detected with remarkably higher level in the BA system than in PS system during the remediation. In addition to the degradation of contaminants, persulfate oxidation promotes microbial bioremediation efficiency mainly by lowering the pH to neutral and increasing the active phosphorus content in the soil. Microbial species and ecological niches were less reduced in the PS system than in the BA system. Collectively, persulfate oxidation had a better impact on the soil microbiome and is more suitable for long-term soil health than bioaugmentation through PheN7 addition.

Graphical abstract

Keywords

Bioaugmentation / Low-dose persulfate oxidation / Polycyclic aromatic hydrocarbon / Remediation

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (Nos. 2020YFC1808801 and 2020YFC1808805) and the National Natural Science Foundation of China (Nos. 41773082 and 41573065).

Ethics Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of Interest

The authors declare no competing interests.

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-023-1733-x and is accessible for authorized users.

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