Innovative method of culturing bdelloid rotifers for the application of wastewater biological treatment

Highlights

• An innovative method of culturing bdelloid rotifer fed on flour was proposed.

• Rotifer fed on flour grew faster than that fed on bacteria or Chlorella vulgaris.

• The optimum mass culture conditions for rotifer fed on flour were investigated.

• The cultured rotifer could improve sludge settleability in the SBR.

Abstract

This study aims to establish a simple and efficient method for the mass culture of bdelloid rotifers, which is the basis for the application of bdelloid rotifers as biological manipulators to improve wastewater biological treatment performance. A common bdelloid rotifer, Habrotrocha sp., in a wastewater biological treatment system was selected as the culture target. Rotifers fed on flour could reproduce faster than those fed traditional food such as Chlorella vulgaris or mixed bacteria. As a rotifer food, flour has the advantages of simple preparation, effortless preservation, and low cost compared to live Chlorella vulgaris or mixed bacteria, so it is more suitable for the mass culture of rotifers. The optimal rotifer culture conditions using flour as food were also studied. According to the experimental results, the recommended rotifer culture conditions are a flour particle size of 1 μm, a flour concentration of 6 × 10⁶ cell/mL, a temperature of 28℃, a pH level of 6.5 and salinity of 100–500 mg/L. In addition, the sludge volume index in the sequencing batch reactor (SBR) with the addition of cultured rotifers was 59.9 mL/g at the end of operation and decreased by 18.2% compared to SBR without rotifer, which indicates that the cultured rotifers still retained the function of helping to improve sludge settling. This function may be related to the rotifer’s role in inhibiting bacteria from producing loosely bound extracellular polymeric substances in the SBR.

Graphical abstract

Keywords

Mass culture / Bdelloid rotifer / Habrotrocha / Wheat flour / Sludge settleability

References

[1] Amaral A L, Leal C S, Vaz A I, Vieira J C, Quinteiro A C, Costa M L, Castro L M (2018). Use of chemometric analyses to assess biological wastewater treatment plants by protozoa and metazoa monitoring. Environmental Monitoring and Assessment, 190(9): 1–15

[2] APHA (1998). Standard Methods for the Examination of Water and Wastewater Analysis.20th ed. Washington DC: American Public Health Association/American Water Works Association/Water Environment Federation

[3] Baer A, Langdon C, Mills S, Schulz C, Hamre K (2008). Particle size preference, gut filling and evacuation rates of the rotifer Brachionus “Cayman” using polystyrene latex beads. Aquaculture (Amsterdam, Netherlands), 282(1–4): 75–82

[4] Błedzki L A, Ellison A M (1998). Population growth and production of Habrotrocha rosa Donner (Rotifera: Bdelloidea) and its contribution to the nutrient supply of its host, the northern pitcher plant, Sarracenia purpurea L. (Sarraceniaceae). Hydrobiologia, 385(1/3): 193–200

[5] Carter B P, Morris C F, Anderson J A (1999). Optimizing the SDS sedimentation test for end-use quality selection in a soft white and club wheat breeding program. Cereal Chemistry, 76(6): 907–911

[6] Deneke R (2000). Review of rotifers and crustaceans in highly acidic environments of pH values≤3. Hydrobiologia, 433(1/3): 167–172 doi:10.1023/A:1004047510602

[7] Ding G, Li X, Lin W, Kimochi Y, Sudo R (2017). Enhanced flocculation of two bioflocculation-producing bacteria by secretion of Philodina erythrophthalma. Water Research, 112: 208–216

[8] Ding G, Zhang Z, He Y, Tang L, Peng L, Cao L, Xu Y, Yi X, Li X, Sudo R (2019). Preliminary study on the function of Philodina sp. in biological wastewater treatment. Acta Scientiae Circumstantiae, 39(10): 3356–3363 (in Chinese)

[9] Hu B, Qi R, Yang M (2013). Systematic analysis of microfauna indicator values for treatment performance in a full-scale municipal wastewater treatment plant. Journal of Environmental Sciences-China, 25(7): 1379–1385

[10] Hu Y Q, Wei W, Gao M, Zhou Y, Wang G X, Zhang Y (2019). Effect of pure oxygen aeration on extracellular polymeric substances (EPS) of activated sludge treating saline wastewater. Process Safety and Environmental Protection, 123: 344–350

[11] Kim H J, Lee J S, Hagiwara A (2018). Phototactic behavior of live food rotifer Brachionus plicatilis species complex and its significance in larviculture: A review. Aquaculture (Amsterdam, Netherlands), 497: 253–259

[12] Lansing A I, Lamy F (1961). Fine structure of the cilia of rotifers. Journal of Biophysical and Biochemical Cytology, 9(4): 799–812

[13] Lapinski J, Tunnacliffe A (2003). Reduction of suspended biomass in municipal wastewater using bdelloid rotifers. Water Research, 37(9): 2027–2034

[14] Leasi F, De Smet W H (2020). Thalassic rotifers from the United States: Descriptions of two new species and notes on the effect of salinity and ecosystem on biodiversity. Diversity (Basel), 12(1):1–26

[15] Lee N M, Welander T (1996). Reducing sludge production in aerobic wastewater treatment through manipulation of the ecosystem. Water Research, 30(8): 1781–1790

[16] Li X Y, Yang S F J W R (2007). Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge. Water Research, 41(5): 1022–1030

[17] Liang P, Huang X, Qian Y, Wei Y S, Ding G J (2006). Determination and comparison of sludge reduction rates caused by microfaunas’ predation. Bioresource Technology, 97(6): 854–861

[18] Ma W C, Zhao L, Liu H L, Liu Q L, Ma J (2017). Improvement of sludge dewaterability with modified cinder via affecting EPS. Frontiers of Environmental Science & Engineering, 11(6): 19

[19] Makareviciene V, Sendzikiene E, Gaide I (2021). Application of heterogeneous catalysis to biodiesel synthesis using microalgae oil. Frontiers of Environmental Science & Engineering, 15(5): 97

[20] Melone G, Ricci C (1995a). Rotatory apparatus in Bdelloids. Hydrobiologia, 313–314(1): 91–98

[21] Melone G, Ricci C (1995b). An uncommon bdelloid rotifer: Abrochtha intermedia (de beauchamp, 1909). Bolletino di zoologia, 62(1): 29–34

[22] Miquelis A, Rougier C, Pourriot R (1998). Impact of turbulence and turbidity on the grazing rate of the rotifer Brachionus calyciflorus (Pallas). Hydrobiologia, 386(1/3): 203–211

[23] Nandini S, Sarma S S S (2001). Population growth of Lepadella patella (O. F. Muller, 1786) at different algal (C. vulgaris) densities and in association with Philodina roseola Ehrenberg, 1832. Hydrobiologia, 446/447: 63–69

[24] Panahi Y, Darvishi B, Jowzi N, Beiraghdar F, Sahebkar A (2016). Chlorella vulgaris: A multifunctional dietary supplement with diverse medicinal properties. Current Pharmaceutical Design, 22(2): 164–173

[25] Pellicer-Nàcher C, Domingo-Félez C, Mutlu A G, Smets B F (2013). Critical assessment of extracellular polymeric substances extraction methods from mixed culture biomass. Water Research, 47(15): 5564–5574

[26] Ponce-Palafox J T, Pavia Á A, Mendoza López D G, Arredondo-Figueroa J L, Lango-Reynoso F, Castañeda-Chávez M D R, Esparza-Leal H, Ruiz-Luna A, Páez-Ozuna F, Castillo-Vargasmachuca S G, Peraza-Gómez V (2019). Response surface analysis of temperature-salinity interaction effects on water quality, growth and survival of shrimp Penaeus vannamei postlarvae raised in biofloc intensive nursery production. Aquaculture (Amsterdam, Netherlands), 503: 312–321

[27] Pous N, Barcelona A, Sbardella L, Hidalgo M, Colomer J, Serra T, Salvado V (2021). Zooplankton-based reactors for tertiary wastewater treatment: A pilot-scale case study. Journal of Environmental Management, 278: 111538

[28] Qu J, Fan M (2010). The current state of water quality and technology development for water pollution control in China. Critical Reviews in Environmental Science and Technology, 40(6): 519–560

[29] Ratsak C H (2001). Effects of Nais elinguis on the performance of an activated sludge plant. Hydrobiologia, 463: 217–222

[30] Ricci C (1984). Culturing of some bdelloid rotifers. Hydrobiologia, 112(1): 45–51

[31] Salvadó H, Palomo A, Mas M, Puigagut J, Gracia M D P (2004). Dynamics of nematodes in a high organic loading rotating biological contactors. Water Research, 38(10): 2571–2578

[32] Sankai T, Ding G, Emori N, Kitamura S, Katada K, Koshio A, Maruyama T, Kudo K, Inamori Y (1997). Treatment of domestic wastewater mixed with crushed garbage and garbage washing water by advanced Gappei-shori Johkaso. Water Science and Technology, 36(12): 175–182

[33] Sarma S S S, Nandini S, Morales-Ventura J, Delgado-Martinez I, Gonzalez-Valverde L (2006). Effects of NaCl salinity on the population dynamics of freshwater zooplankton (rotifers and cladocerans). Aquatic Ecology, 40(3): 349–360

[34] Schaefer E D, Pipes W O (1973). Temperature and the toxicity of chromate and arsenate to the rotifer, Philodina roseola. Water Research, 7(12): 1781–1790

[35] Scragg A, Morrison J, Shales S J E, Technology M (2003). The use of a fuel containing Chlorella vulgaris in a diesel engine. Enzyme and Microbial Technology, 33(7): 884–889

[36] Shiny K J, Remani K N, Nirmala E, Jalaja T K, Sasidharan V K (2005). Biotreatment of wastewater using aquatic invertebrates, Daphnia magna and Paramecium caudatum. Bioresource Technology, 96(1): 55–58

[37] Wang H, Ma D F, Shi W Y, Yang Z Y, Cai Y, Gao B Y (2021). Formation of disinfection by-products during sodium hypochlorite cleaning of fouled membranes from membrane bioreactors. Frontiers of Environmental Science & Engineering, 15(5): 102

[38] Zeng Y, Wei N, Wang Q, Iakovenko N S, Li Y, Yang Y (2020). Bdelloid rotifers (Rotifera, bdelloidea) of China: Diversity and new records. Zookeys, 941: 1–23

[39] Zhang L H, Zheng J, Guo J B, Guan X H, Zhu S Y, Jia Y P, Zhang J, Zhang X Y, Zhang H F (2019). Effects of Al³⁺ on pollutant removal and extracellular polymeric substances (EPS) under anaerobic, anoxic and oxic conditions. Frontiers of Environmental Science & Engineering, 13(6): 85

Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (Grant No. 21777092).

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