Hormesis-like growth and photosynthetic physiology of marine diatom Phaeodactylum tricornutum Bohlin exposed to polystyrene microplastics

Highlights

• Polystyrene microplastic caused hormesis-like effects in Phaeodactylum tricornutum.

• Low concentration of microplastic promoted growth, otherwise the opposite was true.

• The change trends of pigment contents were opposite at two initial algae densities.

• The chlorophyll fluorescence parameters were more sensitive at low algae density.

Abstract

The effects of pristine polystyrene microplastics (pMPs) without any pretreatment at different concentrations (0, 10, 20, 50, and 100 mg/L) on Phaeodactylum tricornutum Bohlin at two initial algae densities (10⁵ and 10⁶ cells/mL) were assessed in this study. Hormesis-like effects were found when microalgae grew with pMPs. The results showed that pMPs inhibited microalgae growth under a high concentration of microplastics tolerated by individual algal cell (low initial algae density) (up to −80.18±9.71%) but promoted growth when the situation was opposite (up to 15.27±3.66%). The contents of photosynthetic pigments including chlorophyll a, chlorophyll c and carotenoids showed resistance to pMPs stress under a low initial algae density and increased with time, but the opposite was true under a high initial algae density. Compared with the low initial algae density group, Qp received less inhibition, and NPQ (heat dissipation) also decreased under the high initial algae density. Under the low initial algae density, OJIP parameters such as S_m, N, Area, Pi Abs, ѱ_o, φ_Eo, TRo/RC and ETo/RC were more perturbed initially and returned to the levels of the control group (without pMPs) over time, but they remained stable throughout the experiment at high initial algae density. These results show that microplastics in the marine environment may have different toxic effects on P. tricornutum at different growth stages, which is of great significance for understanding the impact of microplastics on marine microalgae and aquatic ecosystems.

Graphical abstract

Keywords

Microplastics / Marine microalgae / Phaeodactylum tricornutum Bohlin / Growth / Photosynthetic pigments / Chlorophyll fluorescence parameters

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 52071030) and Science and Technology Program of Henan Province (No.132102310498).

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-021-1436-0 and is accessible for authorized users.

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